2024-02-202024-02-202015Üstüner, B. vd. (2015). "Effect of freezing rate on goat sperm morphology and DNA integrity". Turkish Journal of Veterinary and Animal Sciences, 39(1), 110-114.1300-0128https://doi.org/10.3906/vet-1407-70https://journals.tubitak.gov.tr/veterinary/vol39/iss1/17/https://hdl.handle.net/11452/39869This study investigates the effect of freezing rates on the spermatological parameters of frozen and thawed Saanen goat semen. Equilibrated semen was frozen at 4 different freezing rates from +5 degrees C to -150 degrees C (G10: 10 degrees C/min, G12: 12 degrees C/min, G15: 15 degrees C/min, and G24: 24 degrees C/min) and stored in liquid nitrogen until use. Semen samples were examined for sperm motility, defective acrosomes (FITC-PSA), and DNA integrity using TUNEL after dilution with extender A at equilibration and postthaw stage. There was no significant difference among the freezing stages in terms of DNA fragmentation (P > 0.05). DNA integrity was partially affected by the freezing rate. The increase of freezing rate from 10 degrees C/min to 24 degrees C/min between +5 degrees C and -150 degrees C resulted in higher postthaw DNA damage. The study found that the freeze-thawing process is detrimental to postthawed goat semen motility (P < 0.05), acrosome integrity (P < 0.05), and DNA integrity (P > 0.05). Although the freezing rates used in the present study had no effect on postthaw sperm motility and acrosome integrity (P > 0.05), sperm DNA integrity was affected.eninfo:eu-repo/semantics/openAccessVeterinary sciencesDNA integrityFreezing rateGoat semenCooling rateMotility parametersBoar spermatozoaSemenCryopreservationFragmentationRamFreezabilityPlasmaFrozenArticle0003476786000172-s2.0-84920876763110114391Veterinary sciencesExtender; Semen; Semen extenders