Demirel, Sadettin2024-09-272024-09-272022-12-011098-8823https://doi.org/10.1016/j.prostaglins.2022.106673https://www.sciencedirect.com/science/article/pii/S1098882322000636https://hdl.handle.net/11452/45373Aim/Objective: This study aimed to investigate the effects of Rosa damascena Miller essential oil on rat tracheal smooth muscle contractility and the hypothesis that voltage-gated potassium (KV) channels, ATP-sensitive potassium (KATP) channels, and large-conductance calcium-activated potassium (BKCa) channels may have roles in these effects.Methods: Isometric contraction-relaxation responses of tracheal rings were measured with an isolated tissue bath model. The steady contraction was induced with both 10-5 M ACh and 60 mM KCl, and then the concentrationdependent responses of rose oil (0.1-100 mu g/mL) were examined. The time-matched control (double distilled water) group was also formed. To evaluate the role of KV, KATP, and BKCa channels, tracheal rings were incubated with 4-AP (KV channel blocker), glibenclamide (KATP channel blocker), TEA (BKCa channel blocker), and iberiotoxin (selective BKCa channel blocker). Also, a vehicle control group was formed for dimethyl sulfoxide (DMSO). Results: Rose oil exerted the relaxant effects in tracheal rings pre-contracted with both ACh and KCl at concentrations of 1, 10, and 100 mu g/mL (p < 0.05). Besides, KV channel blocker 4-AP, KATP channel blocker glibenclamide, and BKCa channel blockers TEA and iberiotoxin incubations significantly inhibited the rose oilinduced relaxation responses (p < 0.05). However, incubation of tissues with DMSO, glibenclamide solvent, for 10 min did not cause a significant change in the relaxation responses to rose oil (p > 0.05). Conclusions: In conclusion, the first physiological findings were obtained regarding the functional relaxant effects of rose essential oil in rat trachea. The findings showed that rose oil induces bronchorelaxation in a concentration-dependent manner. Besides, this study is the first to report that rose oil-mediated bronchodilation responses are associated with the activity of KV, KATP, and BKCa channels. These results suggest that rose oil might be a useful agent in the treatment of abnormal bronchoconstriction-related diseases such as asthma and chronic obstructive pulmonary disease.eninfo:eu-repo/semantics/closedAccessAirway smooth-muscleConstituentsInhalationRosa damascenaEssential oilTracheaBronchodilationPotassium channelBiochemistry & molecular biologyCell biologyArticle00087106800000316310.1016/j.prostaglins.2022.106673