2023-04-062023-04-062016-06-30Altınbaş, B. vd. (2016). "Histamine restores hemorrhage induced hypotension by activating cholinergic neurons in nucleus tractus solitarius". Brain Research, 1649(Part A), 132-140.0006-89931872-6240https://doi.org/10.1016/j.brainres.2016.06.047https://www.sciencedirect.com/science/article/pii/S0006899316304723http://hdl.handle.net/11452/32207The purpose of the current study is to investigate the functional connections between the central histaminergic and cholinergic systems at NTS level in hypotensive condition. Experiments were carried out in male Wistar Albino rats. The hypotension was achieved by withdrawing a total volume of 1.5 ml blood/100 g bodyweight over a period of 10 min. A microdialysis study was performed in NTS area to measure extracellular ACh and Ch levels. The hemorrhage produced a severe and long-lasting decrease in mean arterial blood pressure (MAP) and increase in extracellular ACh and Ch levels in NTS. Administration of histamine intracerebroventricularly (i.c.v.) or into the NTS reversed the hemorrhagic hypotension by increasing MAP and heart rate. I.c.v. injection of histamine also caused the additional increase in extracellular ACh and Ch levels. Moreover, central histamine injection augmented intracytoplasmic AChE immunoreactivity in NTS. These changes were completely blocked by histaminergic H1 receptor antagonist chlorpheniramine, but histaminergic H2 receptor blocker ranitidine and histaminergic H3/H4 receptor antagonist thioperamide failed to produce these effects. In conclusion, these findings are interpreted that brain histaminergic H1 receptor activation by central histamine injection may promote cholinergic stimulation in the NTS and subsequently reverses the hypotension.eninfo:eu-repo/semantics/closedAccessNeurosciences & neurologyHistamineNucleus tractus solitariusCentral cholinergic systemCardiovascular systemHemorrhagic hypotensionEndogenous central histamineModulate acetylcholine-releaseDual-probe microdialysisFreely moving ratInduced reversalCardiovascular regulationThromboxane A(2)Arachidonic-acidInjected melittinVentral striatumArticle0003864089000142-s2.0-849883113361321401649Part A27373980NeurosciencesHistamine H4 Receptors; Thioperamide; Intracerebroventricular Drug AdministrationAcetylcholinesteraseChlorpheniramineCholinesteraseHistamineHistamine H1 receptorRanitidineSodium chlorideThioperamideAdultAnimal cellAnimal experimentArticleCardiovascular effectCholinergic nerve cellCholinergic stimulationCholinergic systemControlled studyDrug efficacyExtracellular matrixFunctional connectivityHeart rateHemodynamicsHemorrhagic hypotensionHypotensionImmunohistochemistryImmunoreactivityInjectionMaleMean arterial pressureMicrodialysisNonhumanPriority journalRatSolitary tract nucleus