Escribano, DamianTvarijonaviciute, AstaJoaquin Ceron, JosePardo-Marin, LuisTorrecillas, AlejandroMartinez-Subiela, Silvia2022-11-292022-11-292016-10-10Escribano, D. vd. (2016). "Serum apolipoprotein-A1 as a possible biomarker for monitoring treatment of canine leishmaniosis". Comparative Immunology Microbiology and Infectious Diseases, 49, 82-87.0147-95711878-1667https://doi.org/10.1016/j.cimid.2016.10.002https://www.sciencedirect.com/science/article/pii/S0147957116300984http://hdl.handle.net/11452/29618The aims of this study were: the identification of proteins differentially represented in the serum proteome of dogs with leishmaniosis after treatment and the verification of one selected protein as a possible biomarker for treatment monitoring. Serum samples from five dogs with leishmaniosis, before and after treatment were pooled into two groups and analysed using 2-dimensional electrophoresis followed by mass spectrometry analysis (MS). The MS analysis allowed the identification of 8 proteins differently expressed. APO-A1 was selected and an immunoturbidimetric assay was validated for its measurement in dogs. Significantly decreased concentrations of APO-A1 in dogs with leishmaniosis and a significant increase after a good response to the treatment were observed, suggesting that APO-A1 could be a potential biomarker of treatment monitoring with the advantages of an automated measurement.eninfo:eu-repo/semantics/closedAccessImmunologyMicrobiologyVeterinary sciencesLeishmaniosisDogsApolipoprotein A1Treatment monitoringVisceral leishmaniasisProteinsIdentificationProteomicsDiagnosisAllopurinolAnimalsAntiprotozoal agentsApolipoprotein A-IBiomarkersDogsElectrophoresis, gel, two-dimensionalLeishmaniasisMass spectrometryMeglumineOrganometallic compoundsProteomeArticle0003905218000112-s2.0-8499397054482874927865270ImmunologyMicrobiologyVeterinary sciencesLeishmania Infantum; Dog; PsychodidaeAlpha 1 antitrypsinApolipoprotein A1Biological markerHaptoglobinHemoglobin beta chainImmunoglobulin lambda chainMeglumineRetinol binding protein 4Vitamin D binding proteinAllopurinolAntiprotozoal agentApolipoprotein A1Meglumine antimoniateOrganometallic compoundProteomeArticleBlood samplingCanine leishmaniasisDrug responseLeishmaniasisLimit of detectionMass spectrometryMonitoringNonhumanProtein blood levelProtein expressionProteomicsTwo dimensional electrophoresisValidityAnimalBloodChemistryDogLeishmaniasisTwo dimensional gel electrophoresis