van Wijk, NickBalvers, MartinMaher, Timothy J.Sijben, John W. C.Broersen, Laus M.2022-12-012022-12-012016-02-29van Wijk, N. vd. (2016). "Dietary crude lecithin increases systemic availability of dietary docosahexaenoic acid with combined intake in rats". Lipids, 51(7), 833-846.0024-42011558-9307https://doi.org/10.1007/s11745-016-4139-8https://aocs.onlinelibrary.wiley.com/doi/full/10.1007/s11745-016-4139-8http://hdl.handle.net/11452/29647Crude lecithin, a mixture of mainly phospholipids, potentially helps to increase the systemic availability of dietary omega-3 polyunsaturated fatty acids (n-3 PUFA), such as docosahexaenoic acid (DHA). Nevertheless, no clear data exist on the effects of prolonged combined dietary supplementation of DHA and lecithin on RBC and plasma PUFA levels. In the current experiments, levels of DHA and choline, two dietary ingredients that enhance neuronal membrane formation and function, were determined in plasma and red blood cells (RBC) from rats after dietary supplementation of DHA-containing oils with and without concomitant dietary supplementation of crude lecithin for 2-3 weeks. The aim was to provide experimental evidence for the hypothesized additive effects of dietary lecithin (not containing any DHA) on top of dietary DHA on PUFA levels in plasma and RBC. Dietary supplementation of DHA-containing oils, either as vegetable algae oil or as fish oil, increased DHA, eicosapentaenoic acid (EPA), and total n-3 PUFA, and decreased total omega-6 PUFA levels in plasma and RBC, while dietary lecithin supplementation alone did not affect these levels. However, combined dietary supplementation of DHA and lecithin increased the changes induced by DHA supplementation alone. Animals receiving a lecithin-containing diet also had a higher plasma free choline concentration as compared to controls. In conclusion, dietary DHA-containing oils and crude lecithin have synergistic effects on increasing plasma and RBC n-3 PUFA levels, including DHA and EPA. By increasing the systemic availability of dietary DHA, dietary lecithin may increase the efficacy of DHA supplementation when their intake is combined.eninfo:eu-repo/semantics/openAccessBiochemistry & molecular biologyNutrition & dieteticsPhospholipidsDocosahexaenoic acidPlasma lipidsn-3Fatty acidsNutritionAlgal lipidsFish oilN-3 fatty-acidsLearning-abilityAlzheimers-diseaseMaze behaviorEthyl-esterBrainPlasmaPhosphatidylcholineAbsorptionLipidsAnimalsDietary fats, unsaturatedDietary supplementsDocosahexaenoic acidsDrug synergismEicosapentaenoic acidFatty acids, unsaturatedFish oilsLecithinsMalePlant oilsRatsRats, wistarArticle0003801718000052-s2.0-8496202203783384651727038174Biochemistry & molecular biologyNutrition & dieteticsDocosahexaenoic Acids; Alzheimer Disease; Fish OilsCholineDocosahexaenoic acidFatty acid derivativeFish oilIcosapentaenoic acidMonounsaturated fatty acidOmega 3 fatty acidOmega 6 fatty acidPhosphatidylcholinePolyunsaturated fatty acidSaturated fatty acidVegetable oilDocosahexaenoic acidEdible oilFish oilIcosapentaenoic acidPhosphatidylcholineUnsaturated fatty acidVegetable oilAdultAnimal cellAnimal experimentArticleControlled studyDiet supplementationDietary intakeErythrocyteFatty acid blood levelGas chromatographyLipid compositionMaleNerve cell membraneNonhumanNutrient availabilityRatAnimalBloodChemistryDietary supplementDrug potentiationWistar rat