Jensen, Morten SkovgaardDansher, Gorm2023-03-212023-03-212007-07Noyan, B. vd. (2007). "The lack of effects of zinc and nitric oxide in initial state of pilocarpine-induced seizures". Renewable Energy, 16(5), 410-416.1059-13111532-2688https://doi.org/10.1016/j.seizure.2007.02.012https://www.sciencedirect.com/science/article/pii/S1059131107000532http://hdl.handle.net/11452/31669In this study we investigated whether intracerebroventricular (i.c.v.) injection Of L-NAME (a nitric oxide synthase inhibitor) or CaEDTA (an extracellular zinc chetator) or the combination of the two could affect the initial phase of pitocarpine induced (2 h) seizures. Two groups of rats were used. Animals from both groups were given with i.c.v. injections of either saline (10 mu), L-NAME (150 mu g/10l), CaEDTA (100 mM/10 mu l) or L-NAME and CaEDTA. One group received pilocarpine HCl (380 mg/ kg i.p.) the other served as control. Pilocarpine HCI. was injected intraperitoneally 10 min later. The behavior of the animals was observed for 2 h and the intensity of their seizures was scored. The rats were then sacrificed and their brains were removed and analyzed for zinc ions by using the immersion autometatlography and the TSQ fluorescence staining. All the animals which received pilocarpine HCl developed seizures. Despite treatment with L-NAME and/or CaEDTA we found that the Latency and the intensity of seizures were similar in both groups investigated. The distribution of stainable zinc ions and the intensity of staining in hippocampus were not affected by pilocarpine and found unchanged after L-NAME and/or CaEDTA injections in both the control animals and the pilocarpine treated animals. The data suggest that the nitric oxide system and zinc ions do not affect pilocarpine-induced seizures in their initial state.eninfo:eu-repo/semantics/openAccessImmersion AMGNeurosciences & neurologySynaptically-released zincNitric oxideSeizureTSQ fluorescenceZincMuscarinic receptor subtypesAcid-induced seizuresChelatable zincIn-vivoAnticonvulsantActivationMk-801InjuryZn2+AnimalsDisease models, animalEdetic acidBehavior, animalChelating agentsDrug interactionsNitric oxideEnzyme inhibitorsNG-nitroarginine methyl esterFunctional lateralityInjections, intraventricularHippocampusMalePilocarpineRatsRats, sprague-dawleySeizuresZincArticle0002477269000052-s2.0-3424970337041041616517376708Clinical neurologyNeurosciencesHomocysteine Thiolactone; Thioesters; CystathionineAnimal tissueAnimal behaviorAnimal experimentAnimal modelArticlePilocarpineCombination chemotherapyN(g) nitroarginine methyl esterAutometallographyBrain surgeryClinical observationControlled studyEdetate calciumFluorescence analysisScoring systemDisease severityHippocampusNitric oxideHistochemistryLatent periodSeizureMaleMonotherapyNonhumanPriority journalRatSodium chlorideToluenesulfonamide derivativeZinc