2022-08-122022-08-122015-10Alçay, S. vd. (2015). "Successful ram semen cryopreservation with lyophilized egg yolk-based extender". Cryobiology, 71(2), 329-333.1090-23920011-2240https://doi.org/10.1016/j.cryobiol.2015.08.008https://www.sciencedirect.com/science/article/pii/S0011224015002205http://hdl.handle.net/11452/28186The aim of this study was to evaluate the effects of lyophilized egg yolk extender on ram semen cryopreservation. Ejaculates with a thick consistency, rapid wave motion (3-5 on a 0-5 scale) and >75% initial motility were pooled. Sperm were diluted to final concentration of 1/5 (semen/extender) in lyophilized egg yolk or fresh egg yolk extenders using two-step dilution method. The equilibrated semen was frozen in 0.25 mL straws. Semen samples were assessed for sperm motility, plasma membrane functional integrity using hypoosmotic swelling test (HOST), damaged acrosome using FITC-Pisum sativum agglutinin (PSA-FITC) and DNA integrity using terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) at three time points: after dilution with extender A, equilibration and post-thaw. The results showed that freezing and thawing procedures (dilution, equilibration and thawing) had negative effects on motility (P < 0.001), plasma membrane integrity (P < 0.001), acrosome integrity (P < 0.001) and DNA integrity (P < 0.001). In the study, there were no significant differences between lyophilized and fresh egg yolk extenders when comparing motility, plasma membrane integrity, acrosome integrity and DNA integrity between groups. In conclusion, lyophilized egg yolk extender provided similar cryoprotective effects with fresh egg yolk extender to cryopreserve ram semen.eninfo:eu-repo/semantics/closedAccessLyophilizationEgg yolkRam semenCryopreservationHypoosmotic swelling testSperm membrane integrityIn-vitroSoybean lecithinSpermatozoaFrozenGlycerolFertilityTemperatureParametersLife sciences & biomedicine - other topicsPhysiologyAcrosomeAnimalsCell membraneCryopreservationCryoprotective agentsDNAEgg yolkFreeze dryingFreezingHumansIn situ nick-end labelingMaleSemenSemen preservationSheepSperm motilitySpermatozoaArticle0003623115000172-s2.0-8494229367732933371226277039BiologyPhysiologyExtender; Semen; Semen ExtendersCell DNASperm DNAUnclassified drugCryoprotective agentDNAAcrosomeAnimal cellArticleCell assayCell membraneCell viabilityCellular parametersConcentration (parameters)Controlled studyCryopreservationDilutionEgg yolkFITC conjugatedPisum sativum agglutinin assayFreeze dryingFreeze thawingHypoosmotic stressHypoosmoticSwelling testNonhumanPlasma membrane functional integrityPriority journalSperm preservationSpermatozoon motilityTUNEL assayAnimalCryopreservationDrug effectsEgg yolkFreeze dryingFreezingGeneticsHumanMaleMetabolismPhysiologyProceduresSheepSpermSperm preservationSpermatozoon