Orhan, O.2024-02-162024-02-162014-08Atalay, M. A. vd. (2014). "Glutathione S-transferase M1 and T1 gene polymorphisms are not associated with increased risk of gestational diabetes mellitus development". West Indian Medical Journal, 63(4), 300-306.0043-31442309-5830https://doi.org/10.7727/wimj.2013.128https://myspot.mona.uwi.edu/fms/wimj/article/1803https://hdl.handle.net/11452/39794Aim: The aim of this study was to investigate whether the glutathione S-transferase M1 (GSTM1) and T1 (GSTT1) gene polymorphisms contributed to development of gestational diabetes mellitus (GDM). Subjects and Methods: Fifty women with diagnosis of GDM and 50 control individuals without GDM or altered glucose intolerance during their pregnancy were enrolled in the study. Multiplex polymerase chain reaction-restriction fragment length polymorphism method was applied to determine the GSTM1 and GSTT1 gene polymorphisms. Genotypes were determined according to bands detected with the agarose gel electrophoresis. Results: The difference in the frequencies of GSTM1 null genotypes between GDM and control groups was not statistically significant (60% and 54%, respectively). There was no statistically significant difference between GDM and control groups with respect to GSTT1 null genotype rates (22% and 20%, respectively). Conclusion: This study shows no association between GST gene polymorphisms and GDM.eninfo:eu-repo/semantics/closedAccessGenePolymorphismGestational diabetes mellitusGSTM1Glutathione S-transferaseGSTT1Oxidative stressCancerGeneral & internal medicineGstt1Article0003655679000052-s2.0-8501342141030030663425429472Medicine, general & internalGlutathione S-Transferase M1; Cytochrome P-450 CYP1A1; GenotypeAbortionAdultAgar gel electrophoresisArticleCase control studyClinical articleControlled studyDna polymorphismFamily historyFemaleGene frequencyGenetic riskGenotypeGlucose intoleranceGstm1 geneGstt1 geneHumanMultiplex polymerase chain reactionPregnancyPregnancy diabetes mellitusProspective studyRestriction fragment length polymorphismGlutathione transferase m1Glutathione transferase t1