Satıcıoğlu, İzzet BurçinJanda, J. Michael2022-12-282022-12-282018-09-21Duman, M. vd. (2018). ''The determination of the infectious status and prevalence of motile Aeromonas species isolated from disease cases in rainbow trout (Oncorhynchus mykiss) and aquarium fish''. Journal of Fish Diseases, 41(12), 1843-1857.0140-77751365-2761https://doi.org/10.1111/jfd.12896onlinelibrary.wiley.com/doi/10.1111/jfd.12896http://hdl.handle.net/11452/30150The aims of this study were to determine the prevalence and phylogenetic relationship of motile Aeromonas spp. that might be pathogenic species for rainbow trout in infected/mix infection cases (based upon different outbreaks on fish farms). A total of 99 motile Aeromonas isolates (and three reference strains) were analysed that were isolated from four different fish species in different sizes of fish (0.1-3,000 g), different months and water temperatures (6.1-21.2 degrees C). The biochemical characteristics of the isolates were determined using conventional tests and a rapid test kit. Additionally, molecular identification was performed using the gyrB housekeeping gene region and with glycerophospholipid-cholesterol acyltransferase polymerase chain reaction (GCAT-PCR). The sequencing results obtained from the gyrB gene region were deposited in the GenBank database, and phylogenetic relationships were determined with the BioNumerics 7.6 database. Nearly half of the Aeromonas isolates that were isolated from rainbow trout showing signs of disease were determined to be possible infectious agents. Aeromonas species exhibit biochemical variability for many characters, so some Aeromonas species tested negative for GCAT-PCR despite that this test was created especially for Aeromonas identification. The phylogenetic tree based upon gyrB contained 10 different phylogroups that were based on 96% cut-off value in gyrB gene region.eninfo:eu-repo/semantics/closedAccessFisheriesMarine & freshwater biologyVeterinary sciencesGCAT-PCRMotile aeromonas septicemiaPathogenic aeromonasPhylogenetic treeRibosomal-RNA geneAntibiotic-resistancePhylogenetic analysisVirulence genesIdentificationGyrbWaterPathogencityHydrophilaSPPAcyltransferasesAeromonasAnimalsBacterial proteinsCoinfectionDNA gyraseFish diseasesGram-negative bacterial infectionsOncorhynchus mykissOpportunistic infectionsPhylogenyPolymerase chain reactionPrevalenceTurkeyArticle0004496512000082-s2.0-8505367761518431857411230239011FisheriesMarine & freshwater biologyVeterinary sciencesAeromonas; Aeromonas Hydrophila; VirulenceAdenineAdipic acidCholesterol acyltransferaseCytosineDNA topoisomerase (ATP hydrolysing) BGlycerophospholipidGuaninePhenylacetic acidRNA 16SThymineAcyltransferaseActerial proteinDNA topoisomerase (ATP hydrolysing)Glycerophospholipid-cholesterol acyltransferaseAeromonasAeromonas bestiarumAeromonas caviaeAeromonas dhakensisAeromonas encheleiaAeromonas hydrophilaAeromonas mediaAeromonas molluscorumAeromonas salmonicidaAeromonas veroniiAnimal tissueAquacultureArticleBacterial strainBacterium identificationBacterium isolateBacterium isolationBiochemical analysisBlack SeaCitrobacterCitrobacter gillenii infectionControlled studyData analysis softwareEnteric redmouth diseaseEnterobacter cloacaeEnterobacter cloacae infectionFlavobacterium psychrophilum infectionGene sequenceGoldfishGram negative infectionHousekeeping geneInfectious pancreatic necrosisLactococcus garvieaeLactococcus garvieae infectionMaximum likelihood methodMixed infectionMolecular diagnosisMorganellaMorganella psychrotolerans infectionNonhumanOncorhynchus mykissPhylogenetic treePhylogenyPolymerase chain reactionPrevalenceSalmo truttaWater temperatureZebra fishAeromonasAnimalClassificationFish diseaseGeneticsGram negative infectionMicrobiologyMixed infectionOpportunistic infectionPhysiologyPrevalenceTurkey (bird)Veterinary medicine