2022-12-292022-12-292016-11-25Alçay, S. vd. (2017). ''Royal jelly supplemented soybean lecithin-based extenders improve post-thaw quality and incubation resilience of goat spermatozoa''. Cryobiology, 74, 81-85.0011-2240https://doi.org/10.1016/j.cryobiol.2016.11.011https://www.sciencedirect.com/science/article/pii/S00112240163018081090-2392http://hdl.handle.net/11452/30154The aim of the present study was to evaluate different concentrations of royal jelly (RJ) supplemented extenders for post-thaw quality and incubation resilience of goat spermatozoa. Semen samples were collected from five goats. Pooled semen were diluted with soybean lecithin-based extender without RJ (control) or supplemented with different concentrations (0.25, 0.5 and 0.75%) of RJ (RJ0.25, RJ0.5, RJ0.75 respectively), at a final concentration of 150 x 106 spermatozoon/mL. Semen samples were assessed for sperm motility, plasma membrane integrity using hypoosmotic swelling test (HOST) damaged acrosome using FITC-Pisum sativum agglutinin (PSA-FITC) and DNA integrity using terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL). The addition of RJ (0.5%, 0.75%) led to higher percentages of subjective motilities (55.33 +/- 2.29%, 57.67 +/- 2.58%) compared to control and RJ0.25 groups (49.00 +/- 2,80%, 51.67 +/- 3.09%) (P < 0.05) following the freezethawing process. RJ0.5 and RJ0.75 groups had higher plasma membrane functional integrities (66.40 +/- 1.34%, 68.20 +/- 2.05%) and lower defected acrosome rates (24.60 +/- 3.36%, 23.80 +/- 2.27%) compared to the other groups (P < 0.05). DNA damaged spermatozoa in all groups were not significant (P > 0.05). In the end of incubation, motility and HOST rates of RJ0.5 (14.00 +/- 3.87%, 31.20 +/- 3.70%) and RJ0.75 (15.00 +/- 3.27%, 29.20 +/- 2.59%) groups were higher than control (8.00 +/- 2.54%, 18.20 +/- 3.11%) and RJ0.25 (9.00 +/- 2.07%, 20.60 +/- 2.88%) groups (P < 0.05). Also defected acrosome and DNA fragmation rates of RJ0.5 (32.20 +/- 1.30%, 5.4 +/- 0.55%) and RJ0.75 (29.20 +/- 1.30%, 5.80 +/- 0.45%) groups were significantly lower than control (38.80 +/- 0.84%, 7.40 +/- 1.34%) and RJ0.25 (39.80 +/- 2.05%, 7.00 +/- 1.58) groups. This study shows that RJ supplemented extenders have beneficial effect on goat sperm parameters at 0 h and 6 h of incubation.eninfo:eu-repo/semantics/closedAccessLife sciences & biomedicine - other topicsPhysiologyCryopreservationGoat semenLIncubation resilienceRoyal jellyRam sperm parametersYolk-based extenderEgg-yolkSemen frozenCryopreservationAntioxidantsFertilityPlasmaAcrosomeAnimalsCell membraneCryopreservationCryoprotective agentsDNA damageFatty acidsGoatsIn situ nick-end labelingInsemination, artificialLecithinsMaleSemenSemen preservationSoybean proteinsSperm motilityArticle0003935337000132-s2.0-8500749292381857427908685BiologyPhysiologyExtender; Semen; Semen ExtendersDNAPhosphatidylcholineRoyal jellyCryoprotective agentFatty acidRoyal jellySoybean proteinPhosphatidylcholineArticleCell membraneConcentration (parameters)Controlled studyCryopreservationDNA damageDNA fragmentationFfreeze thawingGoatMaleNonhumanSemen analysisSpermatozoonSpermatozoon motilityAcrosomeAnimalArtificial inseminationCryopreservationDrug effectsPhysiologySpermSperm preservationTUNEL assayVeterinaryArticleCell membraneConcentration (parameters)Controlled studyCryopreservationDNA damageDNA fragmentationFreeze thawingGoatMaleNonhumanSemen analysisSpermatozoonSpermatozoon motilityAcrosomeAnimalArtificial inseminationCryopreservationDrug effectsPhysiologySpermSperm preservationTUNEL assayVeterinary