2022-11-222022-11-222016-03-21Alçay, S. vd. (2016). "Freeze-dried egg yolk based extenders containing various antioxidants improve post-thawing quality and incubation resilience of goat spermatozoa". Cryobiology, 72(3), 269-273.0011-22401090-2392https://doi.org/10.1016/j.cryobiol.2016.03.007https://www.sciencedirect.com/science/article/pii/S0011224016300189http://hdl.handle.net/11452/29527The aim of this study was to evaluate different antioxidants-supplemented freeze-dried egg yolk based extenders for the post-thawing quality and incubation resilience of goat spermatozoa. Pooled semen were diluted in a two-step dilution method to a final concentration of 1/5 (semen/extender) in control and antoxidant supplemented freeze-dried extenders (methionine, cysteamine and butylated hydroxytoluene). Semen samples were assessed for sperm motility, plasma membrane functional integrity using hypoosmotic swelling test (HOST), damaged acrosome using FITC-Pisum sativum agglutinin (PSA-FITC) and DNA integrity using terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL). Membrane lipid peroxidation status was also analyzed using the malondialdehyde (MDA) concentration. In the study, antioxidant supplemented freeze-dried egg yolk based extenders have beneficial effect on goat sperm parameters. In addition, we achieved a higher quality in post thawed goat semen even after 6 h incubation when the extender was supplemented by 5 mM BHT or cysteamine.eninfo:eu-repo/semantics/closedAccessLife sciences & biomedicine - other topicsPhysiologyFreeze-dryingGoat semenCryopreservationAntioxidantsIncubation resilienceRam semen frozenSoybean lecithinSperm parametersCryopreservationFertilityIntegrityAcrosomeAgentsAnimalsAntioxidantsButylated hydroxytolueneCell membraneCryopreservationCysteamineEgg yolkGoatsIn situ nick-end labelingMaleMalondialdehydeSemenSemen preservationSperm motilitySpermatozoaArticle0003778205000142-s2.0-8496228916626927372327018219BiologyPhysiologyExtender; Semen; Semen ExtendersAntioxidantReactive oxygen metaboliteAntioxidantButylcresolMalonaldehydeMercaptamineAcrosomeAnalysis of varianceAnimal cellArticleCell membraneControlled studyCryobiologyCryopreservationDNA fragmentationEgg yolkFertilizationFluorescence microscopyFreeze dryingGoatHypoosmotic swelling testIncubation timeLipid peroxidationMaleMedical proceduresNonhumanOxidative stressPriority journalSpermatozoonSpermatozoon motilityThawingTUNEL assayAnimalDrug effectsMetabolismProceduresSpermSperm preservation