Güler, GülnürAksoy, Kaya2022-09-092022-09-092010-03Çeçener, G. vd. (2010). "FHIT gene sequence variants and reduced fhit protein expression in glioblastoma multiforme". Cellular and Molecular Neurobiology, 30(2), 301-307.0272-43401573-6830https://doi.org/10.1007/s10571-009-9452-9https://link.springer.com/article/10.1007/s10571-009-9452-9http://hdl.handle.net/11452/28616Molecular studies have an important role in the elucidation of the mechanisms involved in Glioblastoma multiforme (GBM) development. The occurrence of FHIT gene alterations, which has an important role in different cancers, has not yet been studied well in GBM. We aimed to investigate the occurrence of alterations of FHIT gene sequence and protein expression in the GBMs. Sequence alterations in exons 5-9 of the FHIT gene were screened in 63 GBMs using the single-strand conformational polymorphism method, followed by DNA sequencing. Additionally, the level of Fhit protein expression in tissues of 48 tumors was assessed by immunohistochemistry (IHC). In our investigation, FHIT gene alterations in the coding region were detected in 11 of the 63 GBM cases (17.5%). Two different sequence variants were determined: one novel missense variant (G -> C transition at codon 49) and one previously described silent alteration (C -> T transition at codon 88). Using web-based programs, such as SIFT and ESEfinder, it was determined that both alterations might have caused significant modification on protein function. In addition, we identified a previously reported an intronic polymorphism (T -> A transition at IVS8-17) in 47.5% of cases as a similar rate (45%) in the control group. Moreover, it was observed that Fhit protein expression was reduced in 87.5% of tumors. In conclusion, the reduction or loss of Fhit protein expression by genetic alterations or epigenetic mechanisms in GBM might be associated with brain tumorigenesis.eninfo:eu-repo/semantics/closedAccessGlioblastoma multiformeFHIT geneSequence alterationsSSCPIHCCommon fragile sitesCell lung-cancerTumor-suppressor geneBrain-tumorsBreast-cancerTurkish patientsPredispositionMutations3P14.2CarcinomaCell biologyNeurosciences & neurologyAcid anhydride hydrolasesAdultAgedBase sequenceBrain neoplasmsFemaleGenetic variationGlioblastomaHumansMaleMiddle agedMolecular sequence dataNeoplasm proteinsPolymorphism, single-stranded conformationalSequence analysis, DNAArticle0002757048000142-s2.0-7795209574130130730219760177Cell biologyNeurosciencesFragile Histidine Triad Protein; Histidine; TriadFragile histidine triad proteinAcid anhydride hydrolaseTumor proteinAdultAgedAmino acid substitutionArticleCarcinogenesisControlled studyDNA sequenceFemaleGene mutationGene sequenceGenetic associationGenetic screeningGenetic variabilityGlioblastomaHumanHuman tissueImmunohistochemistryMajor clinical studyMalePriority journalProtein expressionProtein functionSingle strand conformation polymorphismBrain tumorGenetic variabilityGeneticsGlioblastomaMetabolismMiddle agedMolecular geneticsNucleotide sequence