Erçelen, NesrinAcar, Hasan2021-10-192021-10-192006-05-15Yakut, T. vd. (2006). ''Meiotic segregation analysis of reciprocal translocations, both in sperms and blastomeres''. American Journal of Medical Genetics, 140A(10), 1074-1082.1552-4825https://doi.org/10.1002/ajmg.a.31215https://onlinelibrary.wiley.com/doi/10.1002/ajmg.a.31215http://hdl.handle.net/11452/22404Balanced chromosomal rearrangements could lead to unbalanced segregation gametes during meiosis. In this Study, sperm flourescence ill situ hybridization (FISH) analysis of meiotic segregation products of four reciprocal translocations; 46,XY,t(7;10)(q21;q22), 46:XY,t(15;17)(q11;p12), 46,XY, t(6;13)(p21.1;q32), and 46,XY,t(1;13)(q24;q10) are presented. In three Out Of these four cases with t(15;17), t(6;13), and t(1;13) additional blastomere FISH analyses are also provided. multi-color FISH analysis was applied using diverse probe combinations specific for translocated chromosome segments. The average frequency of sperm nuclei bearing unbalanced products for t(7;10), t(15:17), t(6;13), and t(1;13) were 48.7%, 59.5%, 60.5%, and 62.9%, respectively. Frequencies of blastomeres comprising unbalanced products in Cases with t(15;17), t(6;13), and t(1:13) were 80% (12 of 15), 60% (3 of 5), and 50% (2 of 4), respectively. Chi-square test analysis showed significant differences in the meiotic segregation pattens Clue to the distribution and numbers of the chiasmatas that Could depend oil the size of the translocated segments (P < 0.001). In conclusion, FISH analysis of sperm and blastomere for reciprocal translocation carriers effectively estimates the approximate risk Of unbalanced products and this result might ensure Valuable genetic Counseling.eninfo:eu-repo/semantics/closedAccessGenetics & hereditySpermReciprocal translocationMeiotic segregationBlastomerePatternsDiagnosisAneuploidySpermatozoaFishHeterozygotesCarriersCytogenetic analysisChromosome segregationIn-situ hybridizationTranslocation, geneticSpermatozoaModels, geneticMeiosisMaleKaryotypingInfertility, maleIn situ hybridization, fluorescenceHumansFemaleChromosome segregationBlastomeresArticle0002371711000062-s2.0-3364623029310741082140A1016596678Genetics & heredityRobertsonian Chromosome Translocation; Preimplantation Genetic Diagnosis; Chromosome AberrationsSpermPriority journalMeiosisKaryotype 46, XYHuman cellHumanHeterozygoteGenetic counselingGene probeGameteFluorescence in situ hybridizationControlled studyChromosome translocationChromosome segregationChromosome rearrangementArticleBlastomereCell nucleusChromosome chiasm