Güvenç, FurkanDeniz, Günnur2022-11-292022-11-292016-10-06Budak, F. vd. (2016). "MicroRNA expression patterns of CD8+ T cells in acute and chronic brucellosis". Plos One, 11(11).1932-6203https://doi.org/10.1371/journal.pone.0165138https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0165138http://hdl.handle.net/11452/29625Although our knowledge about Brucella virulence factors and the host response increase rapidly, the mechanisms of immune evasion by the pathogen and causes of chronic disease are still unknown. Here, we aimed to investigate the immunological factors which belong to CD8+ T cells and their roles in the transition of brucellosis from acute to chronic infection. Using miRNA microarray, more than 2000 miRNAs were screened in CD8+ T cells of patients with acute or chronic brucellosis and healthy controls that were sorted from peripheral blood with flow cytometry and validated through qRT-PCR. Findings were evaluated using GeneSpring GX (Agilent) 13.0 software and KEGG pathway analysis. Expression of two miRNAs were determined to display a significant fold change in chronic group when compared with acute or control groups. Both miRNAs (miR-126-5p and miR-47533p) were decreased (p <0.05 or fold change > 2). These miRNAs have the potential to be the regulators of CD8+ T cell-related marker genes for chronic brucellosis infections. The differentially expressed miRNAs and their predicted target genes are involved in MAPK signaling pathway, cytokine-cytokine receptor interactions, endocytosis, regulation of actin cytoskeleton, and focal adhesion indicating their potential roles in chronic brucellosis and its progression. It is the first study of miRNA expression analysis of human CD8+ T cells to clarify the mechanism of inveteracy in brucellosis.eninfo:eu-repo/semantics/openAccessScience & technology - other topicsT-lymphocytesUp-regulationDifferential expressionProstate-cancerDown-regulationPoor-prognosisSerum mirnasHepatitis-BProliferationMelitensisAcute diseaseAdultBrucellosisCD8-positive T-lymphocytesChronic diseaseDisease progressionFemaleGene expression profilingHost-pathogen interactionsHumansImmune evasionMaleMicroRNAsMiddle agedSignal transductionArticle0003876152000142-s2.0-84994735100111127824867Multidisciplinary sciencesAnimals; Zoonosis; Bovine BrucellosisJanus kinaseMicroRNAMicroRNA 126 5pMicroRNA 4753 3pMitogen activated protein kinaseSTAT proteinT lymphocyte receptorUnclassified drugActin filamentAcute diseaseAdultArticleBloodBrucellosisCD8+ T lymphocyteChronic diseaseClinical articleControlled studyDisease courseEndocytosisFemaleFlow cytometryFocal adhesionGene expressionGene functionGene targetingHeredityHumanHuman cellHuman tissueImmune responseMaleMarker geneMicroarray analysisMiddle agedProtein functionProtein protein interactionQuantitative analysisReal time polymerase chain reactionSignal transductionSoftwareWnt signaling pathwayAcute diseaseBrucellosisCD8+ T lymphocyteChronic diseaseGene expression profilingHost pathogen interactionImmune evasionMetabolismPhysiologyProcedures