İkitimur-Armutak, Elif İlkaySönmez, KıvılcımAkgün-Dar, KadriyeŞennazlı, GülbinKapucu, AyşegülYiğit, Funda2022-08-182022-08-182015-03İkitimur-Armutak, E. İ. vd. (2015). "Anticancer effect of a novel palladium-saccharinate complex of terpyridine by inducing apoptosis on ehrlich ascites carcinoma (EAC) in balb-C mice". Anticancer Research, 35(3), 1491-1498.0250-70051791-7530https://ar.iiarjournals.org/content/35/3/1491http://hdl.handle.net/11452/28261Background/Aim: [Pd(sac)(terpy)](sac)•4H2O (sac=saccharinate and terpy=2,2′:6′,2-terpyridine) is newlysynthesized palladium(II) (Pd) complex. We investigated the antiproliferative and apoptotic effects of this complex on Ehrlich ascites carcinoma (EAC). Materials and Methods: EAC cells were administered to 33 Balb/c mice. Mice were divided randomly into four groups: control, cisplatin, Pd(II) complex and paclitaxel. Control group animals received 0.9% NaCl; other groups received treatments cisplatin, Pd(II) complex and paclitaxel on days 7 and 12. At day 14, animals were sacrificed. Expression of active caspase-3, p53 and proliferating cell nuclear antigen (PCNA) was investigated and apoptosis was evaluated by terminal deoxynucleotidyltransferase (TdT)-mediated nick-end labelling (TUNEL) technique. Results: Expression of p53 and PCNA were found to be decreased (p<0.0001), cells with active caspase-3 and TUNEL-positive cells were found to be increased (p<0.0001) in all treatment groups. Conclusion: Like cisplatin and paclitaxel, this Pd(II) complex has a strong anticancer activity against EAC by inducing apoptosis and suppressing proliferation in vivo.eninfo:eu-repo/semantics/closedAccessOncologyPd(II) complexEACIn vivoTumorPCNAApoptosisBiological evaluationCrystal-structureCancerP53ExpressionMechanismsMutationsAnimalsAntineoplastic agentsApoptosisCarcinoma, ehrlich tumorCaspase 3Coordination complexesFemaleMiceMice, Inbred BALB CPalladiumProliferating cell nuclear antigenPyridinesSaccharinTumor suppressor protein p53Article0003524629000342-s2.0-849249424621491149835325750302OncologyComplex; Palladium; 2-PhenylpyridineAntineoplastic metal complexCaspase 3CisplatinCyclineDNA nucleotidylexotransferasePaclitaxelPalladium complexPalladium saccharinateProtein p53Unclassified drugAntineoplastic agentCoordination compoundCyclinePalladiumPyridine derivativeSaccharinAnimal cellAnimal experimentAnimal modelAnimal tissueAntigen expressionAntineoplastic activityAntiproliferative activityApoptosisArticleCell proliferationControlled studyDNA fragmentationEhrlich ascites tumorFemaleImmunohistochemistryIn vivo studyMouseNick end labelingNonhumanPriority journalProtein expressionTumor volumeAnalogs and derivativesAnimalBagg albino mouseCarcinoma, ehrlich tumorChemistryDrug effectsMetabolismPathology