Owen, Medge D.Zaloga, Galy P.Millington, William R.2021-09-012021-09-011997Owen, M. D. vd. (1997). "Glycyl-L-glutamine [beta-endorphin-(30-31)] attenuates hemorrhagic hypotension in conscious rats". American Journal of Physiology-Regulatory Integrative and Comparative Physiology, 273(5), 1598-1606.0363-6119https://journals.physiology.org/doi/full/10.1152/ajpregu.1997.273.5.R1598https://doi.org/10.1152/ajpregu.1997.273.5.r1598http://hdl.handle.net/11452/21589The profound hypotension caused by acute hemorrhage is thought to involve opioid peptide neurons. In this study, we tested whether glycyl-L-glutamine [Gly-Gln; beta-endorphin-(30-31)], a nonopioid peptide derived from beta-endorphin processing, prevents the cardiovascular depression induced by hemorrhage in conscious and anesthetized rats. Previously, we found that Gly-GLn inhibits the hypotension and respiratory depression produced by beta-endorphin and morphine but does not affect opioid antinociception. Hemorrhage (2.5 ml/100 g body wt over 20 min) lowered arterial pressure in conscious rats (from 120.1 +/- 2.9 to 56.2 +/- 4.7 mmHg) but did not change heart rate significantly. Intracerebroventricular Gly-Gln (3, 10, or 30 nmol) pretreatment inhibited the fall in arterial pressure and increased heart rate significantly. The response was dose related and tvas sustained during the 35-min posthemorrhage interval. Pentobarbital sodium anesthesia potentiated the hemodynamic response to hemorrhage and attenuated the effect of Gly-Gln. Gig-Gin (10 or 100 nmol icy) did not influence arterial pressure or heart rate in normotensive rats. These data indicate that Gly-Gln is an effective antagonist of hemorrhagic hypotension.eninfo:eu-repo/semantics/openAccessPhysiologyOpioidPosttranslational processingProopiomelanocortinDipeptideCardiovascularSympathetic-nerve activityBeta-endorphinBlood-pressureAlpha-mshCardiorespiratory depressionHeart-rateNaloxoneMechanismsNorepinephrineReceptorsArticleA1997YF569000062-s2.0-0030695377159816062735PhysiologyAging; Amoxicillin; Contractility InvitroBeta endorphinProopiomelanocortinAnimal cellAnimal experimentAnimal tissueArterial pressureHeart rateHemodynamicsHemorrhagic hypotensionNonhumanPriority journalProtein processingProtein structureRatRespiratory failureStructure activity relation