2022-04-182022-04-182012-05Akkoç, A. vd. (2012). "Matrix metalloproteinase (MMP-2 and MMP-9) and steroid receptor expressions in feline mammary tumors". Biotechnic & Histochemistry, 87(4), 312-319.1052-02951473-7760https://doi.org/10.3109/10520295.2011.652173https://www.tandfonline.com/doi/full/10.3109/10520295.2011.652173http://hdl.handle.net/11452/25820We evaluated the presence of estrogen (ER) and progesterone (PR) receptors, and matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9) enzymes in 18 feline mammary tubulopapillary carcinomas. Immunohistochemistry was performed to localize ER, PR, MMP-2 and MMP-9 in situ. Western blotting and zymographic analyses also were performed to investigate the presence and activities of MMP-2 and MMP-9 enzymes in fresh tissue homogenates. ER immune expression was detected in five samples (27.7%) and PR was positive in sixteen (88.8%) samples. Diffuse cytoplasmic staining of MMP-2 and MMP-9 in neoplastic mammary epithelial cells, stromal fibroblasts and inflammatory cell was evident. MMP-2 and MMP-9 staining was observed also in metastasizing neoplastic cells within lymphatic vessels. MMP-2 and MMP-9 enzymes and their activities in fresh tumor homogenates were demonstrated by zymography. Comparison of MMP-9 gelatinolytic bands from tumor samples and controls revealed a statistically significant difference. We demonstrated elevated MMP-9 and MMP-2 levels in tumor samples by Western blotting; analysis of protein bands revealed 1.9-to-3 fold increase in MMP-9 in tumor samples and the difference was statistically significant. Our results suggest that the expression of MMP-9 can be an important indicator for tumor progression and the possible metastatic nature of feline tubulopapillary carcinomas.eninfo:eu-repo/semantics/closedAccessBiotechnology & applied microbiologyCell biologyEstrogen receptorFeline mammary tumorImmunohistochemistryMatrix metalloproteinaseProgesterone receptorWestern blottingZymographyBreast-cancerEstrogen-receptorsCell-linesInhibitorsCarcinomasCanineAcidDogsFelidaeAnimalsBlotting, westernBreast neoplasmsCat diseasesCatsFemaleGene expression regulation, neoplasticImmunohistochemistryMammary glands, animalMatrix metalloproteinase 2Matrix metalloproteinase 9Receptors, estrogenStaining and labelingUp-regulationArticle0003028091000112-s2.0-8485979068631231987422247959Biotechnology & applied microbiologyCell biologyBreast Tumor; Experimental Mammary Neoplasms; BitchesEstrogen receptorGelatinase aGelatinase bAnimalAnimal diseaseArticleBloodBreast tumorCatCat diseaseEnzymologyFemaleGene expression regulationImmunohistochemistryMetabolismPathologyStainingUdderUpregulationWestern blotting