İkitimur, Elif Armutak2022-09-062022-09-062014-09-01Arı, F. vd. (2014). "Apoptosis-inducing effect of a palladium(II) saccharinate complex of terpyridine on human breast cancer cells in vitro and in vivo". Bioorganic and Medicinal Chemistry, 22(17), 4948-4954.0968-08961464-3391https://doi.org/10.1016/j.bmc.2014.06.039https://www.sciencedirect.com/science/article/pii/S0968089614004854http://hdl.handle.net/11452/28484The anti-growth effect of a palladium(II) complex-[PdCl(terpy)](sac)center dot 2H(2)O] (sac = saccharinate, and terpy = 2,2':6',2 ''-terpyridine)-was tested against human breast cancer cell lines, MCF-7 and MDA-MB-231. Anti-growth effect was assayed by the MTT and ATP viability assays in vitro and then confirmed on Balb/c mice in vivo. The mode of cell death was determined by both histological and biochemical methods. The Pd(II) complex had anti-growth effect on a dose dependent manner in vitro and in vivo. The cells died by apoptosis as evidenced by the pyknotic nucleus, cleavage of poly-(ADP-ribose) polymerase (PARP) and induction of active caspase-3. These results suggest that the palladium(II) saccharinate complex of terpyridine represents a potentially active novel complex for the breast cancer treatment, thus warrants further studies.eninfo:eu-repo/semantics/closedAccessPalladium(II) complexBreast cancerApoptosisCytotoxicityPD(II) complexesDNA bindingDeathCaspase-3CleavageCU(II)PT(II)AssayBiochemistry & molecular biologyPharmacology & pharmacyChemistryMusAnimalsAntineoplastic agentsApoptosisBreast neoplasmsCell proliferationCell survivalCoordination complexesDose-response relationship, drugDrug screening assays, antitumorFemaleHumansMCF-7 CellsMiceMice, inbred BALB CMolecular structurePalladiumPyridinesSaccharinStructure-activity relationshipTumor cells, culturedArticle0003412933000442-s2.0-8490693416449484954221725047941Biochemistry & molecular biologyChemistry, medicinalChemistry, organicComplex; Palladium; 2-PhenylpyridineAdenosine triphosphateAntineoplastic agentCaspase 3CisplatinNicotinamide adenine dinucleotide adenosine diphosphate ribosyltransferasePaclitaxelPalladium complexPalladium saccharinate complexUnclassified drugAntineoplastic agentCoordination compoundPalladiumPyridine derivativeSaccharinAnimal cellAnimal experimentAnimal modelAnimal tissueAntineoplastic activityApoptosisArticleBiochemistryBreast cancerCancer cellCancer inhibitionCell assayCell nucleusCell strainCell strain MDA MB 231Concentration responseControlled studyDose responseDrug structureFemaleHistopathologyHumanHuman cellIn vitro studyIn vivo studyMCF 7 cell lineMouseNonhumanProtein cleavageAnalogs and derivativesAnimalApoptosisBagg albino mouseBreast neoplasmsCell proliferationCell survivalChemical structureChemistryDrug effectsDrug screeningPathologyStructure activity relationSynthesisTumor cell culture