Impact of 3'UTR variation patterns of the KRAS gene on the aggressiveness of pancreatobiliary tumors with the KRAS G13D mutation in a Turkish population

2023-10-122023-10-122016-05-20Egeli, Ü. vd. (2016). "Impact of 3'UTR variation patterns of the KRAS gene on the aggressiveness of pancreatobiliary tumors with the KRAS G13D mutation in a Turkish population". Pancreatology, 16(4), 677-686.1424-39031424-3911https://doi.org/10.1016/j.pan.2016.05.004https://www.sciencedirect.com/science/article/pii/S1424390316300722http://hdl.handle.net/11452/34306Objective: Several studies have demonstrated the importance of mutations in codons 12, 13 and 61 and variations in the 3' untranslated region (3'UTR) of the ICRAS gene, frequently observed genetic events in the progression of pancreatobiliary tumors (PBT). However, limited data exist on the clinical effect of these alterations. The aim of the current study was to clarify the frequency of relevant alterations of the 3'UTR regions of the KRAS gene and the effect of KRAS 3'UTR polymorphisms on the prognosis of patients with codon 12, 13 and 61 mutations in a Turkish population with PBT.Methods: Codons 12, 13, and 61 and 3'UTRs of the KRAS gene were screened by single-strand conformation polymorphism (SSCP) analysis and DNA sequencing in 43 patients and 10 controls. Chi-squared and independent sample T tests were used to evaluate the results of the mutation analysis and clinical features of the patients.Results: We defined the c.38G > A (rs112445441, p.G13D) (39.54%) mutation and two 3'UTR variations, c.*4066delA (rs560890523) (23.26%) and c.*406524066delAA (rs57698689) (6.98%), in the ICRAS gene of Turkish patients. There was a statistically significant relationship between the c.*4066delA (rs560890523) and c.*406524066delAA (rs57698689) variations and invasion and lymph node metastasis status of the patients (p < 0.001). Compared to patients with c.38G > A (rs112445441, p.G13D), patients with c.*4066delA (rs560890523) and c.38G > A (rs112445441, p.G13D) presented more aggressive tumors with highly invasive features. The present study contributes findings regarding the clinical effects of KRAS alterations in PBT. Based on our study, further investigations are required.eninfo:eu-repo/semantics/closedAccessGastroenterology & hepatologyKRAS3' untranslated regionCodon 13MutationInvasionK-ras geneCancerPancreasCodon-12PolymorphismExpressionCarcinomap53AdenocarcinomaMicrornas3' untranslated regionsAdultAgedAged, 80 and overBiliary tract neoplasmsCodonDNA, neoplasmFemaleGene frequencyHumansKaplan-meier estimateLymphatic metastasisMaleMiddle agedMutationNeoplasm invasivenessPancreatic neoplasmsPolymorphism, geneticProto-oncogene proteins p21(ras)TurkeyYoung adultImpact of 3'UTR variation patterns of the KRAS gene on the aggressiveness of pancreatobiliary tumors with the KRAS G13D mutation in a Turkish populationArticle0003902887000302-s2.0-8497513617067768616427256640Gastroenterology & hepatologyPancreatic Neoplasms; Mutation; Chronic PancreatitisGemcitabineKi 67 antigenMicroRNAProtein p533' untranslated regionCodonDNAKRAS protein, humanProtein p213' untranslated regionAdultAgedArticleBile duct tumorBiliary tract tumorClinical articleCodonControlled studyDNA sequenceFemaleGene frequencyGene mutationGenetic variationHumanHuman tissueLymph node metastasisMaleMedian survival timeMutational analysisOncogene K rasPancreas tumorPoint mutationPriority journalSingle strand conformation polymorphismTumor invasionTurk (people)Vater papilla tumor3' untranslated regionBiliary tract neoplasmsGenetic polymorphismGeneticsKaplan Meier methodLymph node metastasisMiddle agedMutationPancreatic neoplasmsTurkeyVery elderlyYoung adult