Ceyhan, MehmetÖzsürekçi, YaseminLucidarme, JayBorrow, RayGürler, NezahatEmiroğlu, Melike KeserÖz, Fatma NurKurugöl, ZaferÇelik, ÜmitParlakay, Aslınur ÖzkayaDinleyici, Ener ÇağrıKarbuz, AdemBelet, NursenDevrim, İlkerGülfidan, GamzeGündeşlioğlu, ÖzlemYücel, MihribanUlusoy, EmelCengiz, Ali Bülent2023-10-232023-10-232020-04-23Ceyhan, M. vd. (2020). "Characterization of invasive Neisseria meningitidis isolates recovered from children in Turkey during a period of increased serogroup B disease, 2013-2017". Vaccine, 38(19), 3545-3552.0264-410X1873-2518https://doi.org/10.1016/j.vaccine.2020.03.024https://www.sciencedirect.com/science/article/pii/S0264410X20303777http://hdl.handle.net/11452/34523Diverse Neisseria meningitidis strains belonging to various serogroups and clonal complexes cause epidemic and endemic life-threatening disease worldwide. This study aimed to investigate the genetic diversity of recent invasive meningococci in Turkey with respect to multilocus sequence type (MLST) and also meningococcal serogroup B (MenB) vaccine antigens to enable assessment of potential MenB strain coverage using the genetic Meningococcal Antigen Typing System (gMATS). Fifty-four isolates, representing 37.5% of all pediatric (ages 0-18 years) invasive meningococcal disease cases in Turkey from January 2013 to December 2017, underwent genome sequence analysis. Thirty-six (66.7%) isolates were MenB, 10 (18.5%) were serogroup W (MenW), 4 (7.4%) were serogroup A (MenA), 3 (5.6%) were serogroup Y (MenY) and 1 (1.8%) was serogroup X (MenX). The MenB isolates were diverse with cc35 (19.4%), cc41/44 (19.4%) and cc32 (13.8%) as the most prevalent clonal complexes. The MenW isolates (n = 10) comprised cc11 (n = 5), ST-2754 (cc-unassigned; n = 4) and cc22 (n = 1). gMATS was indicative of high 4CMenB coverage (72.2-79.1%) of Turkish invasive MenB strains from pediatric patients. Strain coverage of several clonal complexes differed from that seen elsewhere in Europe highlighting the importance of performing local assessments and also the use of phenotypic methods, i.e. MATS, where possible. All of the isolates possessed in-frame fhbp alleles and so were potentially covered by MenB-fHbp. Continued surveillance is essential to guide recommendations for current and future vaccines as well as understanding changes in epidemiology.eninfo:eu-repo/semantics/closedAccessImmunologyResearch & experimental medicineNeisseria meningitidisInvasive meningococcal disease (IMD)GenotypingMolecular epidemiologyTurkeyW135 meningococcal diseaseStreptococcus-pneumoniaeHaemophilus-influenzaeStrain coverageStrain coverageEpidemiologyEmergenceRecommendationsMortalityOutbreakComplexAdolescentAntigens, bacterialChildChild, preschoolEuropeHumansInfantInfant, newbornMeningococcal infectionsMeningococcal vaccinesMultilocus sequence typingNeisseria meningitidisNeisseria meningitidis, serogroup BSerogroupTurkeyArticle0005253181000072-s2.0-8508269333135453552381932199701ImmunologyMedicine, research & experimentalMeningococcosis; Serotype; Neisseria meningitidisComplement factor HMeningococcus vaccineBacterial antigenMeningococcus vaccineAdolescentAdultAlleleArticleBacterial genomeBacterial strainBacterium examinationBacterium isolateChildGenet (clone)Genetic variabilityGenotyping techniqueHumanInfantInvasive speciesMeningococcosisMolecular epidemiologyMultilocus sequence typingNeisseria meningitidisNonhumanPediatric patientPriority journalSequence analysisSerotypeTurkey (republic)Vaccination coverageEuropeGeneticsMeningococcosisNewbornPreschool childTurkey (bird)