Okuturlar, YıldızKeskin, KürşadBurgazlıoğlu, Başak2022-12-122022-12-122015-06-28Bölük, G. vd. (2016). "Comparison of galactomannan, beta-D-glucan, and Aspergillus DNA in sera of high-risk adult patients with hematological malignancies for the diagnosis of invasive aspergillosis". Turkish Journal of Medical Sciences, 46(2), 335-342.1300-01441303-6165https://doi.org/10.3906/sag-1408-100https://journals.tubitak.gov.tr/medical/vol46/iss2/15/http://hdl.handle.net/11452/29803Bckground/aim: Invasive aspergillosis (IA) is a fatal infection that is difficult to diagnose in immunocompromised patients. In this study, Aspergillus-specific DNA was searched using real-time PCR (RT-PCR) in serum samples. Galactomannan (GM) and/or beta-D-glucan (BDG) tests were previously performed on these samples for 70 neutropenic patients with hematological malignancy. Materials and methods: The patients were categorized according to the criteria of the European Organization for Research and Treatment of Cancer/Mycoses Study Group (EORTC/MSG). Among the patient serum samples, the first positive GM or BDG test sample and the median sample of GM or BDG test for negative patients were used to detect DNA levels by RT-PCR method (Light Cycler 480, Roche Molecular Biochemicals, Meylan, France) using a commercial kit (Way2Gene Fungi; Genmar, Izmir, Turkey). Results: When the proven and probable IA group were considered as real patients, sensitivity of Aspergillus-specific DNA test was 90%, specificity was 73.3%, positive predictive value was 81.8%, and negative predictive value was 84.6%. Conclusion: This study found that searching for specific DNA by RT-PCR method has a sensitivity as high as the GM test. Although specificity was rather low, it was concluded that it can be used jointly with GM and BDG tests after decreasing contamination by severe laboratory applications.eninfo:eu-repo/semantics/openAccessGeneral & internal medicineInvasive aspergillosisGalactomannan testBeta-D-glucan testSpecific DNALinked-immunosorbent-assayReal-time PCRPolymerase-chain-reactionCell transplant recipientsFungal-infectionsCirculating galactomannanPulmonary aspergillosisBronchoalveolar lavageClinical-evaluationAntigen-detectionAdultAspergillosisAspergillusDNAHematologic neoplasmsHumansMannansTurkeyArticle0003702711000152-s2.0-8495852576833534246227511494Medicine, general & internalInvasive Aspergillosis; Mycoses; Systemic MycosisAspergillus DNABeta d glucanBeta glucanFungal DNAGalactomannanUnclassified drugDNAGalactomannanMannanAdultArticleComparative studyDNA extractionHematologic malignancyHigh risk patientHumanHuman tissueInvasive aspergillosisMajor clinical studyMicroscopyPredictive valueReal time polymerase chain reactionSensitivity and specificityAspergillosisAspergillusHematologic diseaseTurkey (bird)