Birhanlı, EmreBoran, FilizUlu, AhmetYeşilada, ÖzferAtes, Burhan2024-10-222024-10-222022-04-010045-6535https://doi.org/10.1016/j.chemosphere.2021.133382https://hdl.handle.net/11452/46801This study aims to present a simple and effective carrier matrix to immobilize laccase as opposed to complex and tedious immobilization processes and also to use it in the removal of textile dyes. For this purpose, Cobalt (Co) and Copper (Cu) based metal-organic frameworks (MOFs) were prepared and laccase was immobilized on two different MOFs via encapsulation. The characterization outcomes showed that laccase was well immobilized into MOF supports. Optimum pH and temperature were found for Lac/Co-MOF (pH 4.5 at 50 degrees C) and Lac/Cu-MOF (pH 5.0 at 50 degrees C). The Km (0.03 mM) and Vmax (97.4 mu mol/min) values of Lac/Cu-MOF were lower than those of Lac/Co-MOF (Km = 0.13 mM, Vmax = 230.7 mu mol/min). The immobilized laccases showed good reusability as well as improved resistance to temperature denaturation and high storage stability. For instance, the Lac/Co-MOF and Lac/Cu-MOF retained more than 58% activity after 4 weeks of storage at room temperature. Meanwhile, Lac/Co-MOF and Lac/Cu-MOF maintained 56.5% and 55.8% of their initial activity, respectively, after 12 reuse cycles. Moreover, thermal deactivation kinetic studies of immobilized laccases displayed lower k value, higher t1/2, and enhancement of thermodynamic parameters, which means better thermostability. Finally, the decolorization activities for the Lac/Co-MOF were 78% and 61% at the 5th cycle for Reactive Blue 171 andeninfo:eu-repo/semantics/closedAccessRepeated-batch cultureCovalent immobilizationCrude laccaseCo-mofDecolorizationDegradationEfficientCompositeEnzymePurificationLaccaseEnzyme immobilizationMetal-organic frameworksTextile dyeDecolorizationWastewater remediationScience & technologyLife sciences & biomedicineEnvironmental sciences & ecologyArticle00075831580000729210.1016/j.chemosphere.2021.133382