Tezcan, Gülçin2024-02-162024-02-162020Argadal, Ö. G. vd. (2020). "Long noncoding RNA MALAT1 may be a prognostic biomarker in IDH1/2 wild-type primary glioblastomas". Bosnian Journal of Basic Medical Sciences, 20(1), 63-69.1512-86011840-4812https://doi.org/10.17305/bjbms.2019.4297https://www.bjbms.org/ojs/index.php/bjbms/article/view/4297https://hdl.handle.net/11452/39821Primary glioblastoma (GB) is the most aggressive type of brain tumors. While mutations in isocitrate dehydrogenase (IDH) genes are frequent in secondary GBs and correlate with a better prognosis, most primary GBs are IDH wild-type. Recent studies have shown that the long noncoding RNA metastasis associated lung adenocarcinoma transcript-1 (MALAT1) is associated with aggressive tumor phenotypes in different cancers. Our aim was to clarify the prognostic significance of MALAT1 in IDH1/2 wild-type primary GB tumors. We analyzed IDH1/2 mutation status in 75 patients with primary GB by DNA sequencing. The expression of MALAT1 was detected in the 75 primary GB tissues and 5 normal brain tissues using reverse transcription quantitative PCR (RT-qPCR). The associations between MALAT1 expression, IDH1/2 mutation status, and clinicopathological variables of patients were determined. IDH1 (R132H) mutation was observed in 5/75 primary GBs. IDH2 (R172H) mutation was not detected in any of our cases. MALAT1 expression was significantly upregulated in primary GB vs. normal brain tissues (p = 0.025). Increased MALAT1 expression in IDH1/2 wild-type primary GBs correlated with patient age and tumor localization (p = 0.032 and p = 0.025, respectively). A multivariate analysis showed that high MALAT1 expression was an unfavorable prognostic factor for overall survival (p = 0.034) in IDH1/2 wild-type primary GBs. High MALAT1 expression may have a prognostic role in primary GBs independent of IDH mutations.eninfo:eu-repo/semantics/openAccessLong noncoding RNAMALAT1BiomarkerPrognosisIDH1/2Primary glioblastomaIsocitrate dehydrogenaseHypermethylationResearch & experimental medicineAdultAgedBiomarkers, tumorBrain neoplasmsFemaleGlioblastomaHumansIsocitrate dehydrogenaseMaleMiddle agedMutationRetrospective studiesReverse transcriptase polymerase chain reactionRNALong noncodingRNA, messengerArticle0005129520000082-s2.0-85079075818636920131479414Medicine, research & experimentalIsocitrate Dehydrogenase; Mutation; Epidermal Growth Factor Receptor VIIIIDH1 protein, humanIsocitrate dehydrogenaseIsocitrate dehydrogenase 2HumanLong untranslated RNAMALAT1 long non-coding RNA, humanMessenger RNATumor markerAdultAgedBrain tumorFemaleGeneticsGlioblastomaHumanMaleMetabolismMiddle agedMutationRetrospective studyReverse transcription polymerase chain reaction