Çetinkaya, MerihTayman, CüneytÇekmez, FerhatCanpolat, Fuat EmreTunç, TuranSarıcı, S. Ümit2022-08-192022-08-192013-07Çetinkaya, M. vd. (2013). "Cytidine 5 '-diphosphocholine ameliorates hyperoxic lung injury in a neonatal rat model". Pediatric Research, 74(1), 26-33.0031-3998https://doi.org/10.1038/pr.2013.68https://www.nature.com/articles/pr201368http://hdl.handle.net/11452/28279BACKGROUND: Bronchopulmonary dysplasia (BPD) is an important cause of morbidity. The aim of this study was to evaluate the preventive effect of cytidine 5'-diphosphocholine (CDP-choline) treatment on hyperoxic lung injury in a neonatal rat model. METHODS: A total of 30 newborn pups were divided into control, hyperoxia, and hyperoxia + CDP-choline groups. After birth, pups in the control group were kept in room air and received saline injections, whereas those in hyperoxia and hyperoxia + CDP-choline groups were exposed to 95% O-2 and received daily injections of saline and CDP-choline throughout postnatal day 10, respectively. Histopathological scoring, radial alveolar count, lamellar body membrane protein expression, fibrosis, proinflammatory cytokine levels, lung tissue and bronchoalveolar lavage (BAL) fluid phospholipid content, and apoptosis were evaluated. RESULTS: Hyperoxia-induced severe lung damage was reduced significantly by CDP-choline treatment. Radial alveolar count and lamellar body membrane protein expression were significantly recovered, and the number of terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling-positive cells, active caspase-3 expression, and tissue proinflammatory cytokine levels were decreased by CDP-choline administration. Lung tissue and BAL phospholipid contents showed significant increases after COP-choline administration. CONCLUSION: These data show that COP-choline ameliorates hyperoxic lung injury in a neonatal rat model. It may therefore be suggested that CDP-choline may be a novel therapeutic option for the prevention of BPD.eninfo:eu-repo/semantics/openAccessPediatricsNicotinic acetylcholine-receptorAutonomic nervous-systemBronchopulmonary dysplasiaPreterm infantsCdp-cholineDisaturated phosphatidylcholineSurfactantInflammationApoptosisPulmonaryAnimalsAnimals, newbornCytidine diphosphate cholineDisease models, animalHyperoxiaLung injuryRatsArticle0003217953000052-s2.0-84880291831263374123598810PediatricsCiticoline; Brain Ischemia; GlycerylphosphorylcholineCaspase 3CiticolineCytokineDNA nucleotidylexotransferaseInterleukin 1betaInterleukin 6Membrane proteinPhospholipidTumor necrosis factor alphaAnimal cellAnimal experimentAnimal modelAnimal tissueAntiinflammatory activityApoptosisArticleControlled studyCytokine productionDrug efficacyDrug mechanismEnzyme activationHistopathologyHyperoxiaLamellar bodyLipid peroxidationLung fibrosisLung fluidLung homogenateLung injuryLung lavageLung parenchymaNewbornNonhumanPerinatal periodPriority journalProphylaxisProtein blood levelProtein expressionRat