Kohart, Nicole A.Elshafae, Said M.Demirer, Aylin A.Dirksen, Wessel P.Breitbach, Justin T.Shu, Sherry T.Xiang, JingyuWeilbaecher, Katherine N.Rosol, Thomas J.2024-07-022024-07-022020-01-281042-8194https://doi.org/10.1080/10428194.2019.1672055https://www.tandfonline.com/doi/full/10.1080/10428194.2019.1672055https://hdl.handle.net/11452/42695Parathyroid hormone-related protein (PTHrP) and macrophage inflammatory protein-1 alpha (MIP-1 alpha) are important factors that increase bone resorption and hypercalcemia in adult T-cell leukemia (ATL). We investigated the role of PTHrP and MIP-1 alpha in the development of local osteolytic lesions in T-cell leukemia through overexpression in Jurkat T-cells. Injections of Jurkat-PTHrP and Jurkat-MIP-1 alpha into the tibia and the left ventricle of NSG mice were performed to evaluate tumor growth and metastasis in vivo. Jurkat-pcDNA tibial neoplasms grew at a significantly greater rate and total tibial tumor burden was significantly greater than Jurkat-PTHrP neoplasms. Despite the lower tibial tumor burden, Jurkat-PTHrP bone neoplasms had significantly greater osteolysis than Jurkat-pcDNA and Jurkat-MIP-1 alpha neoplasms. Jurkat-PTHrP and Jurkat-pcDNA cells preferentially metastasized to bone following intracardiac injection, though the overall metastatic burden was lower in Jurkat-PTHrP mice. These findings demonstrate that PTHrP induced pathologic osteolysis in T-cell leukemia but did not increase the incidence of skeletal metastasis.eninfo:eu-repo/semantics/openAccessMacrophage-inflammatory protein-1-alphaSerum-levelsMouse modelHypercalcemiaExpressionPthrpMetastasesHtlv-1Leukemia/lymphomaLocalizationCell lines and animal modelsT-cell leukemiaPthrpMip-1 alphaMetastasisBone resorptionOncologyHematologyArticle00050903780002140941961210.1080/10428194.2019.1672055