Tuna, Bilginer2024-02-132024-02-132014-03-11Üstüner, B. vd. (2014). "Repeated superovulation treatments in Kivircik ewes during breeding and nonbreeding seasons". Turkish Journal of Veterinary and Animal Sciences, 38(5), 480-484.1300-0128https://doi.org/10.3906/vet-1401-20https://journals.tubitak.gov.tr/veterinary/vol38/iss5/4/https://hdl.handle.net/11452/39633The present study compared superovulation and embryo recovery rates applied repeatedly during breeding and nonbreeding seasons in the same 20 Kivircik sheep. Trials 1 and 3 were performed during the breeding season and the trial 2 was performed in the nonbreeding seaSon. We synchronized the sheep's estrus cycles and induced a superovulatory response with porcine follicle stimulating hormone (pFSH) injections. Natural mating took place 12 h after the last FSH injection: The embryos were collected by laparotomy 7 days after natural mating. There was no significant difference between the number of corpora lutea (CL) counted following the 1st (breeding) and 2nd (nonbreeding) superovulatory treatments (P > 0.05). However, the number Of CL in the 3rd (breeding) treatment was dramatically lower than that in the other groups (P < 0.001). Recovery rates showed significant differences among embryo flushing treatments (P < 0.001). The number of morulae + blastocysts and freezable-quality embryos decreased considerably for the 3rd flushing treatment (P < 0.001), while no significant difference was observed between the 1st and 2nd flushing treatments (P > 0.05). In conclusion, our study demonstrated that 2 consecutive in vivo embryo production programs can be successfully applied in Kivircik ewes with suitable superovulation treatments and operation techniques, irrespective of the season.eninfo:eu-repo/semantics/openAccessEmbryoSuperovulationFSHSheepEmbryo-transferRecoveryOvulation rateSheepVeterinary sciencesArticle0003430730000042-s2.0-84906776213480484385Veterinary sciencesEstrus Synchronization; Goat; Estrus