2022-11-222022-11-222016-07-31Budak, F. vd. (2016). "Altered expressions of miR-1238-3p, miR-494, miR-6069, and miR-139-3p in the formation of chronic Brucellosis". Journal of Immunology Research, 2016, 1-11.2314-88612314-7156https://doi.org/10.1155/2016/4591468https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5046029/http://hdl.handle.net/11452/29535Brucellosis is a zoonotic disease that is still endemic in developing countries. Despite early diagnosis and treatment of patients, chronic infections are seen in 10-30% of patients. In this study, we aimed to investigate the immunological factors that play roles in the transition of brucellosis from acute infection into chronic infection. Here, more than 2000 miRNAs were screened in peripheral blood mononuclear cells (PBMCs) of patients with acute or chronic brucellosis and healthy controls by using miRNA array, and the results of the miRNA array were validated through qRT-PCR. Findings were evaluated using GeneSpring GX (Agilent) 13.0 software and KEGG pathway analysis. Four miRNAs were expressed in the chronic group but were not expressed in acute and control groups. Among these miRNAs, the expression level of miR-1238-3p was increased while miR-494, miR-6069, and miR-1393p were decreased (p< 0.05, fold change > 2). These miRNAs have the potential to be markers for chronic cases. The differentially expressed miRNAs and their predicted target genes involved in endocytosis, regulation of actin cytoskeleton, MAPK signaling pathway, and cytokine-cytokine receptor interaction and its chemokine signaling pathway indicate their potential roles in chronic brucellosis and its progression. It is the first study of miRNA expression analysis of human PBMC to clarify the mechanism of inveteracy in brucellosis.eninfo:eu-repo/semantics/openAccessImmunologyDendritic cell maturationB-virus infectionMicrorna expressionGene-expressionUp-regulationIntegrated analysisAbortus invasionDown-regulationCancer cellsTNF-alphaAcute diseaseBiomarkersBrucellosisChronic diseaseFemaleGene expression profilingHumansMaleMetabolic networks and pathwaysMicroRNAsMiddle agedReal-time polymerase chain reactionArticle0003851161000012-s2.0-84990876577111201627722176ImmunologyAnimals; Zoonosis; Bovine BrucellosisCytokineDoxycyclineMicroRNAMicrorna 1238 3pMicrorna 139 3pMicrorna 1914 3pMicrorna 22 5pMicrorna 335 5pMicrorna 339 5pMicrorna 494Microrna 575Microrna 6069Mitogen activated protein kinaseRifampicinUnclassified drugBiological markerMIRN1238 microRNA, humanMIRN139 microRNA, humanMIRN494 microRNA, humanActin filamentAdultArticleBrucellosisChronic brucellosisChronicityClinical articleControlled studyDisease courseEndocytosisFatigueFemaleGene expressionHumanHuman cellImmune responseMalePeripheral blood mononuclear cellProtein protein interactionReverse transcription polymerase chain reactionSignal transductionAcute diseaseBloodBrucellosisChronic diseaseGene expression profilingGeneticsImmunologyMetabolismMicrobiologyMiddle agedReal time polymerase chain reaction