Asian Pacific Journal of Cancer Prevention, Vol 15, 2014 10899 DOI:http://dx.doi.org/10.7314/APJCP.2014.15.24.10899 Role of PET/CT in Treatment Planning of Head and Neck Cancer Patients Undergoing Definitive Radiotherapy Asian Pac J Cancer Prev, 15 (24), 10899-10903 Introduction Positron emission tomography performed using 18F-deoxyglucose (FGD-PET) is a hybrid method that can yield both functional and anatomic data and has been widely used in the last decade. This method is based on the fact that glucose consumption is increased in tumor cells. (Chapman et al., 2003). FDG PET scans are commonly used for the staging and restaging of various malignancies, such as head and neck, breast, colorectal and gynecological cancers (Kang et al., 2014). Innovations in computer technology have allowed us to combine PET and computed tomography (CT) images i.e. positron emission tomography/computed tomography (PET/CT). PET/CT fused image was obtained, achieved the matching of anatomical and functional details, and may reflect both the biological morphological changes in early detection of disease (Tan et al., 2014). Radiotherapy (RT) planning computers can use the 1Department of Radiation Oncology, 4Department of Medical Oncology, Diyarbakir Gazi Yasargil Education and Research Hospital, Diyarbakir, 2Department of Radiation Oncology, 3Department of Nuclear Medicine, Uludag University, Faculty of Medicine, 5Department of Radiation Oncology, Bursa Ali Osman Sonmez Oncology Hospital, Bursa, Turkey *For correspondence: onurilyasoglu131202@yahoo.com Abstract Background: In this study, we aimed to investigate the benefits of 18F-deoxyglucose positron emission tomography/computed tomography (FGD-PET/CT) imaging for staging and radiotherapy planning in patients with head and neck cancer undergoing definitive radiotherapy. Materials and Methods: Thirty-seven head and neck cancer patients who had undergone definitive radiotherapy and PET/CT at the Uludag University Medical Faculty Department of Radiation Oncology were investigated in order to determine the role of PET/CT in staging and radiotherapy planning. Results: The median age of this patient group of 32 males and 5 females was 57 years (13-84years). The stage remained the same in 18 cases, decreased in 5 cases and increased in 14 cases with PET/CT imaging. Total gross tumor volume (GTV) determined by CT (GTVCT-Total) was increased in 32 cases (86.5%) when compared to total GTV determined by PET/CT (GTVPET/CT-Total). The GTV of the primary tumor determined by PET/CT (GTVPET/CT) was larger in 3 cases and smaller in 34 cases compared to that determined by CT (GTVCT). The GTV of lymph nodes determined by PET/CT (GTVLNPET/CT) was larger in 20 cases (54%) and smaller in 12 cases (32.5%) when compared to GTV values determined by CT (GTVLNCT). No pathological lymph nodes were observed in the remaining five cases with both CT and PET/ CT. Conclusions: We can conclude that PET/CT can significantly affect both pretreatment staging and assessed target tumor volume in patients with head and neck cancer. We therefore recommend examining such cases with PEC/CT before treatment. Keywords: Head and neck cancer - PET-CT - radiotherapy RESEARCH ARTICLE Role of PET/CT in Treatment Planning for Head and Neck Cancer Patients Undergoing Definitive Radiotherapy Sonay Arslan1, Candan Demiroz Abakay2, Feyza Sen3, Ali Altay2, Tayyar Akpinar3, Ahmet Siyar Ekinci4, Onur Esbah4*, Nuri Uslu1, Kezban Esra Kekilli5, Lutfi Ozkan2 PET/CT fusion technique to combine CT and PET images one-to-one. Thus, nodal detection and describing tumor limits has become easier and more accurate with the assessment of matching images. There are several studies indicating that using PET imaging with conventional techniques like x-ray, CT, or MRI can have a significant effect on gross tumor volume (GTV), clinical target volume (CTV) and planning target volume (PTV) (Demir et al., 2009). In this study, we aimed to investigate the benefits of PET/CT on the staging and radiotherapy planning steps in head and neck cancer patients who had undergone definitive radiotherapy. Materials and Methods Thirty-seven head and neck cancer patients who had undergone definitive radiotherapy and routine PET/CT before treatment planning were investigated Sonay Arslan et al Asian Pacific Journal of Cancer Prevention, Vol 15, 201410900 retrospectively in order to determine the role of PET/CT in treatment planning. This group consisted of 32 male and 5 female cases with a median age of57 years (13-84years). Primary tumor locations were the nasopharynx in 12 cases, the oropharynx in 7 cases, the hypopharynx in 5 cases and the larynx in 9 cases. Histopathological examination of tumor tissues defined23 cases as squamous cell carcinoma and non-keratinizing undifferentiated carcinoma in 10 cases. Five cases graded as 3 and 9 cases as grade 4; 23 patients could not be graded. Definitive radiotherapy was decided on after examining each patient based on clinical findings, physical examination findings, pathology examinations and radiological findings. After the first assessment, a head and neck mask was applied at the treatment position for all patients. Patients with prior PET/CT examinations underwent a CT simulation. Patients without prior PET/ CT examination were performed PET/CT simulation after preparing thermoplastic mask. Patients lied down in supine position to device table for CT imaging. Flat carbon fiber treatment table was used in both devices in order to provide the same conditions in both CT and PET/ CT. Thermoplastic head and neck mask was prepared for all patients with neck support parts made of foam. Patient’s arms were stabilized with shoulder tractors. Tongue depressor was used in patients with tumor localized in mouth floor and 2/3 anterior of the tongue. Center of the scanning area is defined as the overlapping point of horizontal, vertical and longitudinal lasers which are placed in the CT simulation device as the same of the treatment room. Small lead markers were placed to each laser point on the individuals in order to determine reference slice of the CT slices. Topogram images were taken for determining the related area. Head and neck area was imaged by 2 slice multidetector spiral CT with the section thickness of 3 mm. The routine PET/CT imaging protocol was applied in all cases. PET images with attenuation correction were transferred in DICOM (Digital Imaging and Communications in Medicine) format to the contouring computer at the Uludag University Medical Faculty Department of Radiation Oncology. PET images and CT images were matched using the contouring computer. Cases were initially staged according to American Joint Committee on Cancer (AJCC) 2010 TNM classification criteria (AJCC, 2010) based on clinical and CT findings. Afterwards, they staged patients based on clinical and PET/CT findings and the two stages were compared. Patients who had undergone neo-adjuvant chemotherapy were staged based on pre-treatment (on diagnosis) CT and PET/CT findings. The contouring process was performed by the same radiation oncology specialist. The GTV of the tumor and the GTV of head and neck lymph nodes (LN) were contoured based only on CT images (GTVCT and GTVLNCT, respectively). CT images and PET/CT images were matched using the contouring computer by radiation oncology and nuclear medicine specialists by choosing the image fusion technique. Afterwards, the GTV of the primary tumor and head and neck lymph nodes were contoured by the same radiation oncology and nuclear medicine physicians based on images created by the fusion of PET and CT images (GTVPET/CT and GTVLNPET/CT respectively). Lymph nodes which could not be differentiates by physicians as the primary tumor or metastatic lymph nodes were also included in the GTVCT or GTVPET/CT. Lymph nodes >1 cm were defined as pathological. In the PET/CT images, standardized uptake value (SUVmax) ≥2 lymph nodes were included in the GTVLN. GTVs imaged via PET/CT were contoured as a visible area according to the International Commission on Radiation and Measurements (ICRU) 62 (Suit H, 1996). GTVCT and GTVLNCT determined via CT and GTVPET/ CT and GTVLNPET/CT determined via PET/CT were transferred to a treatment planning computer. Data were recorded in cm3 using volume calculating algorithms in the treatment planning system. GTVCT-Total was calculated by adding GTVCT to GTVLNCT and GTVPET/CT- Total was calculated by adding GTVPET/CT to GTVLNPET/ CT. The results were recorded in cm3. Mathematical volume changes were calculated. The SPSS (Statistical Packages for the Social Sciences, IBM) program was used for statistical analysis. The Wilcoxon signed rank-test was used to compare GTVPET/ CT-Total and GTVCT-Total with GTV and GTVLN values obtained by CT and PET/CT individually. Pearson correlation analysis was used to compare GTVPET/CT-Total with GTVCT-Total, GTVPET/BT with GTVCT and GTVLNPET/ CT with GTVLNCT individually. The McNemar-Bowkertest was used to analyzethe stage changes between Tstage-ct and Tstage-pet/ct, Nstage-ct and Nstage-pet/ct, Mstage-ct and Mstage-pet/ct, Totalstage-ct and Totalstage-pet/ct. The Pearson correlation test was used for correlation analysis. Statistical significance was defined as p<0.05. Results Analysis of Stage Alteration. Stage changes were Table 1. Statistical Analysis of Volume Values Median (Min-max) p* r p-value** GTV PET/CT 32.71 (3.14-311.23) <0.001 0.792 <0.001 CT 55.77 (7.16-390.13) GTVLN PET/CT 7.49 (0-114.46) 0.217 0.777 <0.001 CT 5.29 (0-126) GTVTotal PET/CT 50.90 (4.80-311.23) <0.001 0.762 <0.001 CT 81.98 (7.60-390.13) *r:Correlation coefficient *Wilcoxon test **Pearson correlation analysis; GTV: Gross tumor volume; GTVLN:Involved locoregional lymph node volume; GTVLNtotal: Total volume of primary tumor+locoregional lymph nodes; CT: Computed tomography; PET/CT: Positron emission tomography/computed tomography Asian Pacific Journal of Cancer Prevention, Vol 15, 2014 10901 DOI:http://dx.doi.org/10.7314/APJCP.2014.15.24.10899 Role of PET/CT in Treatment Planning of Head and Neck Cancer Patients Undergoing Definitive Radiotherapy observed with PET/CT in 19 of 37 patients and the treatment plan was changed afterwards. There was no statistically significant difference between Tstage-CT and Tstage-PET/CT (p=0.306). However, there was a positive correlation between Tstage-CT and Tstage-PET/CT (r=0.664; p<0.001). When comparing the T, N and M parameters of the TNM staging system based on CT and PET/CT, the T parameter was increased in 8 cases, decreased in 8 cases and unchanged in the remaining 21 patients with PET/CT imaging. There was no statistically significant difference between Nstage-CT and Nstage-PET/CT (p=0.197). However, there was a positive correlation between Nstage-CT and Nstage-PET/CT (r=0.556; p<0.001). When comparing the PET/CT and CT results, it was observed that the N parameter was decreased in 2 cases, increased in 10 cases and unchanged in the remaining 25 patients. It was also observed that in spite of stage not changes N parameter in nine cases, new LN areas were discovered by PET/CT and added to the treatment plan. Pathological lymph nodes (>1 cm) detected by CT were excluded from the treatment plan in nine cases due to alack of metabolic activity based on the PET/CT data. There was no statistically significant difference between Mstage-CT and Mstage-PET/CT (p=0.125). However, there was a positive correlation between Mstage-CT and Mstage-PET/CT (r=0.543; p<0.001). In four cases (11%), asymptomatic metastatic disease was detected with PET/CT examination findings despite a lack of CT findings. In one case, a lymph node was located at the anterior mediastinum; in two cases bone metastases and in one case bone and lung metastases were detected with PET/CT. The McNemar-Bowker test could not be applied to Totalstage-PET/CT since there was no stage 1 case. However, a positive correlation was observed between Totalstage-CT and Totalstage-PET/CT (r=0.424; p=0.009).When PET/CT and CT imaging methods were compared on a stage basis, it was observed that PET/CT did not affect the stage in 18 cases, increased the stage in 14 cases and decreased the stage in 5 cases. The PET/CT method changed the stage in almost half of the cases. Analysis of Volume Alteration. GTVCT-Total was found to be increased in 32(86.5%) cases and decreased in 5 cases when compared to GTVPET/CT-Total. GTVPET/CT was found to be increased in tree cases and decreases 34 cases when compared to GTVcT. GTV and GTVLN volume values were also compared. GTVLNPET/CT was found to be increased in 20 cases (54%) and decreased in 12 cases (32.5%) when compared to GTVLNCT. No pathological lymph nodes were detected in the remaining five cases. A statistically significant difference was observed with the Pearson correlation test when comparing the GTV, GTVLN and GTV Total volumes calculated based on CT and PET/CT imaging methods. The Wilcoxon test showed statically significant difference between GTV and GTV Total while GTVLN was not significantly different (Table 1). Discussion Significant changes have happened recently with the increased use of PET/CT, a functional imaging method. It is accepted as a standard method only for staging and radiotherapy of lung cancer for now; however, there are ongoing studies on other cancer types (Kim et al., 2007). In a study of Mutlu H et al. reported that staging with PET CT has better results in terms of survival staging. This superiority leads to survival advantage in patients with locally advanced non small cell lung cancer (Mutlu et al., 2013). It is still not a standard method for head and neck cancers, but has been used widely. It has been shown that PET/CT is superior to classical methods (CT, MRI and USG) for tumor characterization and may change the treatment methods (Koc et al., 2011). In a study of De Antonio et. al. it was stated that TNM stage and clinical stage were altered with the PET/CT in 22 % of the cases (Deantonio et al., 2008). Connel et. al conducted a study consisting 76 patients in order to assess the effects of PET/CT on prognosis; and reported TNM stage alteration in 34% of the cases based mostly on N parameter (Connell et al., 2007). Guido et al., also reported that 6 of 38 head and neck cancer patients’ stage was altered after PET/CT and they also mentioned that these alterations based on the changes in nodal stage. In this study it was observed that nodal stage was increased in 5 cases and decreased in 1 case with PET/ CT. Furthermore; new nodal activity was observed in 9 cases without changing the stage even these lymph nodes were not observed with CT imaging (Guido et al., 2009). In our study T stage was increased in 8 cases, decreased in 8 cases and was not changed in 21 cases ; N stage increased in 10 cases, decreased in 2 cases and was not changed in 25 cases with PET/CT. In 4 cases determined as M0 before; distant metastasis was detected with PET/ CT. TNM stage was increased in 14 cases, decreased in 5 cases and was not changed in 18 patients. In a meta-analysis conducted in order to define the role of PET/CT for detecting distant metastases, it was stated that PET/CT is superior to other imaging methods (Xu et al., 2011). We also observed distant metastases in four cases which we were unable to detect with other imaging methods. In radiotherapy, it is crucial to contour critical organs and target organs for the treatment plan. The most important reason that PET/CT is a preferred method over others for contouring target volumes is that it can provide better differentiation of tumor tissue from healthy tissue (McGuirt et al., 1995). Comparing target tumor volumes determined by CT and PET/CT images may Figure 1. Radiotherapy Planning Volumes Described by Planning CT and PET/CT. GTV: Gross tumor volume GTVLN: Involved locoregional lymph node volume; CT: Computed tomography; PET/CT: Positron emission tomography/ computed tomography Sonay Arslan et al Asian Pacific Journal of Cancer Prevention, Vol 15, 201410902 result in changing the treatment plan. There are four main methods for GTV contouring using PET/CT, although these methods are not standardized (Paulino et al., 2004; Nestle et al., 2005;). i) Visual assessment; ii) Contouring based on a certain SUVmaxisocontour; iii) Contouring based on a certain proportion of the SUVmaxvalue; iv) Contouring based on source/basis activity ratio. Visual assessment is usually used with PET/CT imaging; we also prefer to use the visual method for volume calculation. Most researchers consider target volume changes described by PET or CT. There are studies reporting both an increase and a decrease in the target volume (Daisne et al., 2004; Heron et al., 2004; Van Baardwijk, 2004; Paulino et al., 2005; Schwartz et al., 2005). In the literature, this change in the GTV is reported as mostly decreasing. Daisneet al. compared the calculated volumes of 19 head and neck cancer patients using CT, MRI and PET/CT and reported that there was no significant difference between the GTV values described by CT and MRI. However, the GTV values calculated using PET were found to be decreased in patients with tumors in the larynx, oropharynx and hypopharynx (Daisne JF et al. 2004). Heron et al. reported that the GTV of primary tumors was decreased in 14 cases and increased in 3 cases (a total of 21 cases) using PET/CT (Heron DE, et al. 2004). In a study by Van Baardwijket al. consisting 16 patients, comparing GTVPET/CT and GTVCT results, it was found that the GTVPET/CT values were significantly smaller (Van Baardwijk, 2004). Paulinoet al. compared GTVPET/CT and GTVCT and reported a 75%decrease in 40 cases (Paulino et al., 2005). In a study by Scwartzet al consisting of 19 cases and in a study by Scarfone et al. consisting of 9 cases, it was reported that there were no significant differences between GTVPET and GTVCT values in both studies (Scarfone et al., 2004; Schwartz et al., 2005). In our study, GTVPET/CT was smaller than GTVCT in 34 (92%) of 37 cases, while GTVCT was smaller than GTVPET/CT in 3 cases. Heron et al. investigated 21 patients and detected metastatic lymph nodes in 15 cases. They reported that nodal volume parameter was decreased in 3 cases, increased in 9 cases and was unchanged in 3 cases. They also stated that there was no significant difference in terms of nodal involvement areas (Heron DE, et al. 2004). In our study, GTVLNPET/CT was larger in 20 cases (54%) and smaller in 12 cases (32.5%) when compared to GTVLNCT. There was no statistically significant difference between GTVLNPET/CT and GTVLNCT. 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