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Title: Effect of experimental endotoxemia on thrombelastography parameters, secondary and tertiary hemostasis in dogs
Authors: Failing, Klaus H.
Moritz, Andreas
Bauer, Natali B.
Uludağ Üniversitesi/Veterinerlik Fakültesi/Dahiliye Anabilim Dalı.
Eralp, Oya
Yılmaz, Zeki
Keywords: Veterinary sciences
Acute phase reaction
Disseminated intravascular coagulation
Protein C
Protein S
Systemic inflammatory response syndrome
Disseminated intravascular coagulation
Activated protein-C
Septic shock
Reference intervals
Severe sepsis
Canis familiaris
Issue Date: 2011
Publisher: Wiley
Citation: Eralp, O. vd. (2011). "Effect of experimental endotoxemia on thrombelastography parameters, secondary and tertiary hemostasis in dogs". Journal of Veterinary Internal Medicine, 25(3), 524-531.
Abstract: Background Thrombelastography (TEG) and indicators of secondary and tertiary hemostasis might be altered in dogs with endotoxemia. Hypothesis Endotoxemia influences measures of coagulation in dogs. Animals Ten healthy cross-bred dogs. Material and Methods Prospective laboratory study between controls (n = 5) receiving 0.9% saline IV and the study group (n = 5) treated with low-dose lipopolysaccharide (0.02 mg/kg IV). Physical examination and sampling for measurement of leukocytes, platelets, and coagulation variables were performed at time points 0, 1, 4, and 24 hours. Coagulation variables included kaolin-activated TEG, 1-stage prothrombin time (OSPT), activated partial thromboplastin time (aPTT), fibrinogen, factor VIII, antithrombin, protein C, protein S, activated protein C (APC)-ratio calculated from aPTT with and without presence of APC), and D-Dimers. Results Endotoxemia-induced clinical signs included lethargy (n = 5/5), diarrhea (n = 4/5), emesis (n = 4/5), and abdominal pain (2/5). After 1 hour there was severe leukopenia (2.5 +/- 0.7 x 109/L; mean +/- SD, P < .0001) and a 2.2-fold increase in D-Dimers (0.81 +/- 0.64 mg/L, P < .0001). After 4 hours there was hyperthermia (40.3 +/- 0.4 degrees C, P < .0001) and increases in OSPT (10.5 +/- 2.7 seconds, P < .0001), aPTT (16.7 +/- 5.2 seconds, P = 0.002). A significant decrease in fibrinogen (1.5 +/- 1.0 g/L, P = 0.001), protein C (31 +/- 33%, P <.0001), protein S (63 +/- 47%, P < .0001), TEG alpha (58 +/- 19, P = .007), and TEG maximal amplitude (50 +/- 19 mm, P = .003) was seen compared with the controls. APC-ratio rose significantly (2.5 +/- 0.2, P < .0001) without exceeding the reference interval (n = 4/5). Conclusion and Clinical Importance D-Dimers are the earliest indicator for endotoxemia-associated coagulation abnormalities followed by decreased protein C concentration. APC-ratio and TEG were not good screening variables.
ISSN: 0891-6640
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Web of Science

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