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Title: Comparison of in vitro gas production technique with in situ nylon bag technique to estimate dry matter degradation
Authors: Kamalak, Adem
Bursa Uludağ Üniversitesi/Ziraat Fakültesi/Hayvancılık Bölümü.
Canbolat, Önder
Gürbüz, Yavuz
Özay, Özkan
Keywords: In vitro gas production
In situ dry matter degradability
Rumen fermentation
Medicago sativa
Triticum aestivum
Zea mays
Issue Date: Feb-2005
Publisher: Czech Academy Agricultural Sciences
Citation: Kamalak, A. vd. (2005). "Comparison of in vitro gas production technique with in situ nylon bag technique to estimate dry matter degradation". Czech Journal of Animal Science, 50(2), 60-67.
Abstract: Dry matter (DM) degradation of wheat straw (WS), barley straw (BS), lucerne hay (LH) and maize silage (MS) was determined using two different techniques: (i) in vitro gas production and (ii) nylon bag degradability technique. In vitro gas production and in situ DM disappearance were measured after 3, 6, 12, 24, 48, 72 and 96 hours of incubation. In situ and in vitro DM degradation kinetics was described using the equation y = a + b (1 - e(ct)). In all incubations there were significant (P < 0.001) correlations between gas production and in situ DM disappearance or estimated parameters ((a + b)(gas) and (a + b)(is) or (a + b)(gas) and EDMDis) whereas there were no significant (P > 0.05) correlations between c(gas) and c(is) or b(gas) and b(is). Gas production from the insoluble fraction (b) alone explained 98.3 % of the variation of EDMD. The inclusion of gas production from the quickly soluble fraction (a) and rate constant (c) of gas production in the regression equation improved the accuracy of EDMD prediction. The correlations between the results of both methodologies seem to be, sufficiently strong to predict degradability parameters from gas production parameters. It was concluded that the in vitro gas production technique has good potentiality to predict in situ DM disappearance and some DM degradation parameters.
ISSN: 1212-1819
Appears in Collections:Scopus
Web of Science

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