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Title: L-glutamine enemas attenuate mucosal injury in experimental colitis
Authors: Uludağ Üniversitesi/Tıp Fakültesi/Cerrahi Anabilim Dalı.
Uludağ Üniversitesi/Tıp Fakültesi/Biyokimya Anabilim Dalı.
Uludağ Üniversitesi/Tıp Fakültesi/Patoloji Anabilim Dalı.
Kaya, Ekrem
Gür, Esma Sürmen
Özgüç, Halil
Bayer, Ahmet
Tokyay, Rıfat
Keywords: Gastroenterology & hepatology
Oxidant injury
Short chain fatty acids
Chain fatty-acids
Inflammatory bowel-disease
Distal ulcerative-colitis
5-aminosalicylic acid
Colonic mucosa
Rat colon
Butyrate metabolism
Rectal irrigation
Issue Date: 1999
Publisher: Lippincott Williams & Wilkins
Citation: Kaya, E. vd. (1999). "L-glutamine enemas attenuate mucosal injury in experimental colitis". Diseases of the Colon & Rectum, 42(9), 1209-1215.
Abstract: PURPOSE: The aim of this study was to investigate the role of l-glutamine, short chain fatty acid, prednisolone, and mesalazine (5-aminosalicylic acid) enemas on mucosal dam age and inflammation in experimental colitis. METHODS: Colitis was induced in rats with trinitrobenzene sulfonic acid in ethanol. Saline (n = 14), prednisolone (n = 13), 5-aminosalicylic acid (n = 14), l-glutamine (n = 14), and shea chain fatty acid (n = 13) enemas were applied twice daily to the rats for seven days after the induction of colitis. The sham group (n = 9) received only saline enemas. Rats were killed at the seventh day and their colonic macroscopic inflammatory scores were determined. Colonic mucosal gamma glutamyl transpeptidase activity and colonic mucosal malondialdehyde levels were measured. The same measurements but no enemas were done in the control group (n = 7). RESULTS: There were significant differences in macroscopic inflammatory scores between sham and colitis groups (P < 0.001). The macroscopic inflammatory scores of the colitis group were higher than the short chain fatty acid and glutamine groups (P < 0.05). Whereas the mucosal gamma glutamyl transpeptidase activity was diminished in prednisolone, 5-aminosalicylic acid, and short chain fatty acid groups when compared with the control group; in the colitis, sham, and glutamine groups the activity of this enzyme did not change. The mucosal malondialdehyde levels were significantly lower in the prednisolone and glutamine groups than in the colitis group. CONCLUSION: Only one of four agents tested, namely, l-glutamine enemas, could decrease the severity of colitis both morphologically and biochemically. Moreover, L-glutamine prevented the colitis-induced oxidant injury in the colonic mucosa. On the other hand, prednisolone and short chain fatty acids seemed to improve only the physiologic changes of colitis.
Description: Conference: 8th European Congress of Surgery Location: Budapest, Hungary Date: Jul 17-20, 1998
ISSN: 0012-3706
Appears in Collections:Web of Science

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