In vitro phannacodynaniics of enrofloxacin against an Escherichia coli gyrA mutant
Date
2011
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Medwell Online
Abstract
The aim of this study was to investigate in vitro the pharmacodynamics of Enrofloxacin (ENR) against an Escherichia coli gyrA mutant (E. coli MT/128). Broth microdilution testing was used to determine the Minimum Inhibitory Concentration (MIC) and multi-step resistance selection was performed until reaching 1 mu g ML(-1) MIC. For time-kill experiments, colony counts were determined by plating each diluted sample onto Plate Count Agar and an integrated pharmacokinetic/pharmacodynamics area measure (log ratio area) was applied to all cfu data. A single C-T mutation was found in gyrA at codon 83. Concentration-dependent bacterial killing was observed for E. coli MT/128. Bactericidal activity for this strain was achieved within 4 h at concentrations >= 8 times the MIC with no significant regrowth by 24 h.
Description
Keywords
Veterinary sciences, Pharmacodynamic of enrofloxacin, Escherichia coli, C-T mutation, Significant, Microdilution, Turkey, Colony-variant phenotype, Fluoroquinolone resistance, Staphylococcus-aureus, Streptococcus-pneumoniae, Quinolone resistance, Mechanisms, Mutations, Selection, Animals, Model
Citation
Cengiz, M. vd. (2011). "In vitro phannacodynaniics of enrofloxacin against an Escherichia coli gyrA mutant". Journal of Animal and Veterinary Advances, 10(18), 2384-2388.