Matrix metalloproteinase (MMP-2 and MMP-9) and steroid receptor expressions in feline mammary tumors
Date
2012-05
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Taylor & Francis
Abstract
We evaluated the presence of estrogen (ER) and progesterone (PR) receptors, and matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9) enzymes in 18 feline mammary tubulopapillary carcinomas. Immunohistochemistry was performed to localize ER, PR, MMP-2 and MMP-9 in situ. Western blotting and zymographic analyses also were performed to investigate the presence and activities of MMP-2 and MMP-9 enzymes in fresh tissue homogenates. ER immune expression was detected in five samples (27.7%) and PR was positive in sixteen (88.8%) samples. Diffuse cytoplasmic staining of MMP-2 and MMP-9 in neoplastic mammary epithelial cells, stromal fibroblasts and inflammatory cell was evident. MMP-2 and MMP-9 staining was observed also in metastasizing neoplastic cells within lymphatic vessels. MMP-2 and MMP-9 enzymes and their activities in fresh tumor homogenates were demonstrated by zymography. Comparison of MMP-9 gelatinolytic bands from tumor samples and controls revealed a statistically significant difference. We demonstrated elevated MMP-9 and MMP-2 levels in tumor samples by Western blotting; analysis of protein bands revealed 1.9-to-3 fold increase in MMP-9 in tumor samples and the difference was statistically significant. Our results suggest that the expression of MMP-9 can be an important indicator for tumor progression and the possible metastatic nature of feline tubulopapillary carcinomas.
Description
Keywords
Biotechnology & applied microbiology, Cell biology, Estrogen receptor, Feline mammary tumor, Immunohistochemistry, Matrix metalloproteinase, Progesterone receptor, Western blotting, Zymography, Breast-cancer, Estrogen-receptors, Cell-lines, Inhibitors, Carcinomas, Canine, Acid, Dogs, Felidae
Citation
Akkoç, A. vd. (2012). "Matrix metalloproteinase (MMP-2 and MMP-9) and steroid receptor expressions in feline mammary tumors". Biotechnic & Histochemistry, 87(4), 312-319.