Serum butyrylcholinesterase and paraoxonase 1 in a canine model of endotoxemia: Effects of choline administration
Date
2012-10
Authors
Tvarijonaviciute, Asta
Tecles, Fernando
Ceron, Jose J.
Journal Title
Journal ISSN
Volume Title
Publisher
Elsevier Science
Abstract
Butyrylcholinesterase (BChE) and paraoxonase 1 (PON1) are two serum enzymes synthesized by the liver that are related with inflammation. The main objectives of this study were to determine changes in serum BChE and PON1 by using a canine model of endotoxemia, and to evaluate whether choline alters BChE and PON1 activities during inflammation. For this purpose, a total of 20 mongrel dogs were divided into four groups: control, choline (C), lipopolysaccharide (LPS), and LPS + C. Dogs in the control group were injected with 0.9% NaCl (0.2 ml/kg, i.v.). Dogs in C and LPS + C groups received choline chloride (20 mg/kg, i.v., three times with 4 h intervals). Endotoxin was injected (0.02 mg/kg, i.v., once) to the dogs of LPS and LPS + C groups. Statistically significant decreases in BChE and PON1 activities in LPS group were detected 24 and 48 h post injection, respectively. No statistically significant changes in BChE and PON1 activities at different times were detected in control, C, or LPS + C groups. In conclusion, the data obtained in present study revealed a decrease in serum BChE and PON1 activities in dogs during experimentally induced endotoxemia and that choline administration attenuates these changes.
Description
Keywords
Veterinary sciences, BChE, Choline, Dog, Lipopolysaccharide, Pon1, S1rs, Cdp-choline, Whole-blood, Involvement, Metabolites, Insulin, Sepsis, Inhibition, Receptors, Decrease, Rats
Citation
Tvarijonaviciute, A. vd. (2012). "Serum butyrylcholinesterase and paraoxonase 1 in a canine model of endotoxemia: Effects of choline administration". Research in Veterinary Science, 93(2), 668-674.