Effect of low temperature thawing procedure and post-thaw cold shock on frozen bull semen
Date
2005-09
Authors
Ak, Kemal
İleri, İrfan Kamuran
Journal Title
Journal ISSN
Volume Title
Publisher
Polish Society of Veterinary Sciences
Abstract
The objective of this study was to investigate the effect of low temperature thawing and post-thaw cold shock application on sperm motility, as well as acrosomal and plasma membrane integrities of cryopreserved bull semen. Frozen semen was thawed in a water bath at 5-7 degrees C for 30, 60 or 90 s (low thawed groups), cold shocked (at 5-7 degrees C for 30, 60, 90 s) after thawing at 37 degrees C for 30 s (cold shocked groups), cold for 30 s (control group). The thawing procedure affected the percentages of motile spermatozoa (P < 0.001)and damaged acrosome (P < 0.001) while the bull factor affected the percentages of motile spermatozoa (P < 0.01) and damaged acrosome (P < 00.01). However, swollen tail spermatozoa rates were not affected by the the two factors. There was a significant interaction between thawing procedures and bulls in terms of the studies sperm parameters (P < 0.001). In conclusion, the percentages of motility, intact acrosome and swollen tail spermatozoa of the control group were higher than low-temperature thawed and post-thaw cold-shocked groups. These results indicate that frozen spermatozoa are significantly sensitive to lower temperature not only before but also after thawing.
Description
Keywords
Veterinary sciences, Bull, Semen, Acrosome, Membrane functional integrity, Sperm membrane, Bovine spermatozoa, Hypoosmotic test, Ram, Viability, Damage
Citation
Nur, Z. vd. (2005). "Effect of low temperature thawing procedure and post-thaw cold shock on frozen bull semen". Medycyna Weterynaryjna, 61(9), 991-993.