Resistance to chlorpyrifos and lambda-cyhalothrin along with detoxifying enzyme activities in field-collected female populations of European red mite
Date
2009-02
Authors
Susurluk, Hilal
Gürkan, Mehmet Oktay
Journal Title
Journal ISSN
Volume Title
Publisher
Springer
Abstract
The levels of susceptibility of populations of the European red mite Panonychus ulmi (Koch) (Acarina: Tetranychidae) collected from apple orchards in the Bursa region of Turkey to the insecticides chlorpyrifos and lambda-cyhalothrin, were determined by a petri leaf disk-Potter spray tower method. When compared with the susceptible population, resistance ratios at the LC50 level ranged from 6.0- to 35.6-fold, and from 0.7- to 5.7-fold for chlorpyrifos and lambda-cyhalothrin, respectively. Kinetic parameters of general esterase activity with alpha-naphthyl acetate as substrate indicated that an increased activity was present in the resistant populations compared with the susceptible populations. In these strains, 1.5- and 2.2-fold higher Glutathione S-transferase (GST) activity was also detected with the substrate 1-chloro-2,4-dinitrobenzene. General esterase activity gel profiles of these populations were studied by native polyacrylamide gel electrophoresis, but no relationship between resistance ratios and band patterns was detected. The results of this study document a decreased efficacy of chlorpyrifos and lambdacyhalothrin in field populations of P. ulmi in Turkey, possibly linked to altered activities of esterases and GST.
Description
Keywords
General esterase, Glutathione S-transferase, Insecticide resistance, Organophosphates, Panonychus ulmi, Synthetic pyrethroids, 2-spotted spider-mite, Glutathione-s-transferase, Ulmi koch acari, Tetranychus-urticae acari, Panonychus-ulmi, Different strains, Cross-resistance, Susceptibility, Detoxification, Inheritance, Agriculture, Plant sciences, Entomology, Acari, Malus x domestica, Panonychus ulmi, Tetranychidae
Citation
Kumral, N. A. vd. (2009). "Resistance to chlorpyrifos and lambda-cyhalothrin along with detoxifying enzyme activities in field-collected female populations of European red mite". Phytoparasitica, 37(1), 7-15.