Tip 1 diyabet (T1D)’li türk hastalarda glutatyon-S-transferaz (GSTT1 ve GSTM1) gen polimorfizmlerinin araştırılması
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Date
2012-03-22
Authors
Karkucak, Mutlu
Cander, Soner
Gül, Özen Öz
Journal Title
Journal ISSN
Volume Title
Publisher
Uludağ Üniversitesi
Abstract
Oksidatif stres, tip 1 diyabet (T1D) ve komplikasyonlarının gelişiminde önemli bir rol oynamaktadır Oksidatif stresin zararlı etkilerine karşı savunma sistemlerinden biri de Glutatyon-S-Transferaz (GST)’dır. Çalışmamızda, GSTT1 ve GSTM1 gen polimorfizmleri ile T1D’li Türk hastalar arasındaki ilişkiyi araştırmayı amaçladık. Çalışmamıza, T1D tanısı konmuş 71 hasta ile 62 kontrol birey dahil edildi. GSTM1 ve GSTT1 gen polimorfizmini değerlendirmek için multiplex PCR yöntemi kullanıldı. İstatistiksel analizde anlamlılık düzeyi p <0.05 olarak belirlendi. GSTT1 ve GSTM1 negatif(null) genotipleri istatistiksel olarak değerlendirildiğinde, gruplar arasında anlamlı bir fark saptanmadı (p>0.05). Bu sonuçlar GSTT1 ve GSTM1 negatif(null) genotiplerinin T1D ve komplikasyonların gelişimi için katkısının olmadığını göstermektedir fakat daha geniş olgu sayılı çalışmalara ihtiyaç vardır.
Oxidative stress plays an important role in the development of type 1 diabetes(T1D) and its complications. Glutathione-S-Transferases (GST) is one of the defense systems against the harmful effects of oxidative stress. In our study, we aimed to investigate the relationship between GSTT1 and GSTM1 gene polymorphisms and Turkish patients with T1D. In our study, 71 patients were diagnosed with T1D and 62 control subjects were included. GSTM1 and GSTT1 gene polymorphisms were used to evaluate the multiplex PCR method.In statistical analysis, the level of significance was set at p<0.05. GSTT1 and GSTM1-negative (null) genotypes were evaluated statistically, no significant difference was found between the groups (p>0.05). These results show that GSTT1 and GSTM1-negative (null) genotypes may not contribute to the development of T1D and its complications but more studies with larger sample size are needed.
Oxidative stress plays an important role in the development of type 1 diabetes(T1D) and its complications. Glutathione-S-Transferases (GST) is one of the defense systems against the harmful effects of oxidative stress. In our study, we aimed to investigate the relationship between GSTT1 and GSTM1 gene polymorphisms and Turkish patients with T1D. In our study, 71 patients were diagnosed with T1D and 62 control subjects were included. GSTM1 and GSTT1 gene polymorphisms were used to evaluate the multiplex PCR method.In statistical analysis, the level of significance was set at p<0.05. GSTT1 and GSTM1-negative (null) genotypes were evaluated statistically, no significant difference was found between the groups (p>0.05). These results show that GSTT1 and GSTM1-negative (null) genotypes may not contribute to the development of T1D and its complications but more studies with larger sample size are needed.
Description
Keywords
Polimorfizm, Tip 1 diyabet, GSTT1 geni, GSTM1 geni, Polimorphism, Type 1 diabetes, GSTT1 gene, GSTM1 gene
Citation
Karkucak, M. vd. (2012). "Tip 1 diyabet (T1D)’li türk hastalarda glutatyon-S-transferaz (GSTT1 ve GSTM1) gen polimorfizmlerinin araştırılması". Uludağ Üniversitesi Tıp Fakültesi Dergisi, 38(2), 75-78.