Cryptosporidiosis’li buzağılarda inek kolostrumunun tedavideki etkinliğinin ve kan serumundaki proteomik yapı üzerine etkisinin değerlendirilmesi
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Date
2020-12-15
Authors
Kaçar, Yiğit
Journal Title
Journal ISSN
Volume Title
Publisher
Bursa Uludağ Üniversitesi
Abstract
Cryptosporidiosisli buzağılarda tedavi amaçlı kolostrumun, bikarbonatlı veya bikarbonatsız şekilde paramomisin ile kombine edilmesinin, etkinliği ve serum proteomik profiline etkisinin araştırılması amaçlanmıştır. Ayrıca ilk kez yüksek ve düşük kaliteli kolostrumların proteomik analizlerle karşılaştırılması amaçlanmıştır. Çalışmada, yalnızca Cryptosporidium ile enfekte, 21 günden küçük 30 Holştayn buzağı üç eşit gruba ayrılmıştır. Her üç gruba 1x100 mg/kg dozunda paramomisin (5 gün) ilave olarak, Grup 1 ve 2’ ye 2x250 ml kolostrum (3 gün) ve Grup 1’e kolostrum öncesinde 2x6 g sodyum bikarbonat uygulanmıştır. Tedavi öncesinde ve sonrasındaki 10 gün buzağıların klinik muayeneleri, dışkı skorlaması ve ookist sayımları yapılmıştır. Tedavinin 0, 1, 2, 3, 5 ve 7. gün serumlarından Brix% ve total protein (TP), 0 ve 3. günde immunglobulin G (IgG), gamma-glutamyltransferase (GGT) ölçümleri ve proteomik analizler yapılmıştır. Dışkı skorları, Grup 1 ve 2’ de 2. günde tedavi öncesine göre belirgin şekilde (p<0,05) azalırken, Grup 3 (kontrol grubu)’te bu fark 8. günde belirginleşmiştir. Benzer şekilde ookist sayıları Grup 1’de 2. gün, Grup 2’de 3. gün düşmesine karşın Grup 3’te ancak 10. günde fark belirlenmiştir (p<0,05). Kolostrum tedavisine rağmen IgG, TP, Brix% ve GGT düzeylerinin azalması büyük moleküllü proteinlerin yeterince emilmediğini; kolostral tedavi ile Grup 1’de A1AT, SAA, ACTG, HP20; Grup 2’de ise A1BG, S39AB, CTHL1, ACTB APOA4 proteinlerinde değişim olması, ancak Grup 3’te hiçbir proteinde anlamlı değişim olmaması tedavide kolostrum kullanımının buzağı serum proteomiğine etkileri olduğunu göstermiştir. Ayrıca, yüksek kaliteli kolostrumda 8, düşük kaliteli kolostrumda ise 11 protein daha yüksek konsantrasyonda tespit edilmiştir. Sonuçta, cryptosporidiosis tedavisinde paramomisine ilaveten özellikle bikarbonat ve kolostrum kullanımının daha iyi sonuç verdiği gözlenmiştir.
Aim of the presented study was investigate the effect of using colostrum combined with or without bicarbonate in addition to paramomycin, on the treatment outcome and serum proteomic profiles in calves with cryptosporidiosis. Inaddition, it was aimed to compare proteomic analysis results of high and low quality colostrums for the first time. Thirty Holstein calves younger than 21 days that were infected only with Cryptosporidium spp. were allocated into three equal groups. Paramomycin was administered 1x100 mg / kg for 5 days to all three groups. Additionally 250 ml colostrum was fed twice a day for 3 days to Groups 1 and 2. Additionally Group 1 recieved 6g oral sodium bicarbonate twice a day before colostrum administration. Clinical examinations, fecal scoring and oocyst counts of calves were conducted before and 10 days following the initiation of treatment. Brix% and total protein (TP) levels were analysed from sera at day 0, 1, 2, 3, 5 and 7 days; immunoglobulin G (IgG), gamma-glutamyltransferase (GGT) measurements and proteomic analyzes were performed at day 0 and 3 day. Fecal scores decreased significantly (p <0,05) on the second day of the treatment in Group 1 and 2 comparing to pretreatment levels. But same decrement was not observed in Group 3 (control group) till the 8thday of the treatment. Similarly, oocycts counts decreased in Group 1 and Group 2 respectively on 2ndand 3rdday of the treatment however decrement was detected in Group 3 on the 10thday (p<0,05). Decrement of IgG, TP, Brix% and GGT showed that large mollecules were not absorbed enough in spite of colostrum treatment. Colostral treatment used in Group 1 and 2 changed the levels of A1AT, SAA, ACTG, HP20 proteins and A1BG, S39AB, CTHL1, ACTB APOA4 proteins respectively but there was no differences for protemic structure in Group 3. Results showed that using colostrum in the treatment effects the calf serum proteomic. Inaddition, 8 proteins in high quality and 11 proteins in low quality colostrum were detected high concentration. In conclusion, using paramomycin with bicarbonate and colostrum gave better outcome in cryptosporidiosis treatment.
Aim of the presented study was investigate the effect of using colostrum combined with or without bicarbonate in addition to paramomycin, on the treatment outcome and serum proteomic profiles in calves with cryptosporidiosis. Inaddition, it was aimed to compare proteomic analysis results of high and low quality colostrums for the first time. Thirty Holstein calves younger than 21 days that were infected only with Cryptosporidium spp. were allocated into three equal groups. Paramomycin was administered 1x100 mg / kg for 5 days to all three groups. Additionally 250 ml colostrum was fed twice a day for 3 days to Groups 1 and 2. Additionally Group 1 recieved 6g oral sodium bicarbonate twice a day before colostrum administration. Clinical examinations, fecal scoring and oocyst counts of calves were conducted before and 10 days following the initiation of treatment. Brix% and total protein (TP) levels were analysed from sera at day 0, 1, 2, 3, 5 and 7 days; immunoglobulin G (IgG), gamma-glutamyltransferase (GGT) measurements and proteomic analyzes were performed at day 0 and 3 day. Fecal scores decreased significantly (p <0,05) on the second day of the treatment in Group 1 and 2 comparing to pretreatment levels. But same decrement was not observed in Group 3 (control group) till the 8thday of the treatment. Similarly, oocycts counts decreased in Group 1 and Group 2 respectively on 2ndand 3rdday of the treatment however decrement was detected in Group 3 on the 10thday (p<0,05). Decrement of IgG, TP, Brix% and GGT showed that large mollecules were not absorbed enough in spite of colostrum treatment. Colostral treatment used in Group 1 and 2 changed the levels of A1AT, SAA, ACTG, HP20 proteins and A1BG, S39AB, CTHL1, ACTB APOA4 proteins respectively but there was no differences for protemic structure in Group 3. Results showed that using colostrum in the treatment effects the calf serum proteomic. Inaddition, 8 proteins in high quality and 11 proteins in low quality colostrum were detected high concentration. In conclusion, using paramomycin with bicarbonate and colostrum gave better outcome in cryptosporidiosis treatment.
Description
Keywords
Buzağı, Cryptosporidiosis, Kolostrum, Proteomik analiz, Paramomisin, Calf, Cryptosporidiosis, Colostrum, Proteomic analysis, Paramomycin
Citation
Kaçar, Y. (2020). Cryptosporidiosis’li buzağılarda inek kolostrumunun tedavideki etkinliğinin ve kan serumundaki proteomik yapı üzerine etkisinin değerlendirilmesi. Yayınlanmamış doktora tezi. Bursa Uludağ Üniversitesi Sağlık Bilimleri Enstitüsü.