2013 Cilt 7 Sayı 20

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  • ItemOpen Access
    Isolation of pyridine degrading bacteria from soils contaminated with petrochemical industry effluents in purba medinipur
    (Uludağ Üniversitesi, 2013-08-07) Chatterjee, Shamba; Biswas, Soumik; Ganguly, Samrat; Mathur, Anil Kumar
    Pyridine and its derivatives are of major concern as environmental pollutants due to their toxic nature. Bioremediation is considered to be an alternative process to clean up the environment polluted with heterocyclic pollutants including pyridine and its derivatives. Our primeval objectives were first to isolate the pyridine degrading bacteria from the petrochemical industry effluent enriched soil sample collected from effluent discharge site in Purba Medinipur, West Bengal and further to explore morphological, biochemical, molecular characteristics and the optimum conditions under which these strains can most efficiently breakdown pyridine. In this study, one new bacterial strain, Shewanella algae (designated as MM) capable of utilizing pyridine as a sole carbon source was isolated from the contaminated site and we have explored different research approaches alongwith 16S rDNA gene analysis method for unveiling this intended purpose. Furthermore, it can be used effectively for the treatment of pyridine bearing wastewater and as a possible inoculum in a biofilter treating pyridine-laden gas.
  • ItemOpen Access
    Phenological stages of medlar (mespilus germanica l. ‘istanbul’) according to the bbch scale
    (Uludağ Üniversitesi, 2013-08-07) Atay, Ersin
    The BBCH scales are wellknown in phenology studies and practical for the communication between different scientific disciplines. In this study, extended BBCH scale was used to describe the phenological growth stages of ‘İstanbul’ medlar variety. The BBCH scale for medlar can be used widely by researchers as well as agrochemical companies. This is the first study on analysis of medlar phenological development using BBCH scale.
  • ItemOpen Access
    Influence of foliar application of 5-sulfosalicylic acid, malic acid, putrescine and potassium nitrate on vegetative growth and reproductive characteristics of Strawberry cv. ‘Selva’
    (Uludağ Üniversitesi, 2013-08-14) Kazemi, Mohsen
    In order to study the effect of foliar application of 5-sulfosalicylic acid, malic acid, putrescine and potassium nitrate on vegetative growth and reproductive characteristics of strawberry cv. Selva as a factorial experiment in a completely randomized design with 4 replications which the factors included 5-sulfosalicylic acid at three levels (0.5, 1.5 and 2.5 mM), malic acid at three levels (1.5, 2.5 and 5 mM), putrescine at two levels (1 and 2 mM) and potassium nitrate at two levels (1 and 2 %). As result has shown 5sulfosalicylic acid at 2.5 mM and potassium nitrate at 2% concentration increased vegetative growth (dry weight, leaf area, length of roots), number of flowers, and weight of primary and secondary. 5-sulfosalicylic acid at 2.5 mM and malic acid at 5 mM of increased total phenolics, flavonoids, and non-flavonoids of strawberry but other treatments did not have a significant impact on this characteristic. The highest percentage of total soluble solids, titratable acidity and ascorbic acid was attained in fruits treated with 2.5 mM of foliar 5-sulfosalicylic acid and the lowest, was achieved in control
  • ItemOpen Access
    In vitro culture of gisela 6 semi-dwarf rootstock
    (Uludağ Üniversitesi, 2013-06-03) Aghaye, Rahim Nazary Moghaddam; Yadollahi, Abbas; Moeini, Ahmad; Sepahvand, Sadegh
    This research was carried out to study micropropagation of Gisela 6 rootstock in order to achieve suitable media for producing 5 thousand plants. Explants were cultured in MS and WPM media containing 2 types of cytokinins BAP and TDZ at different concentrations. The results showed that the number of shoots in MS medium containing 1.2 mg L BAP was higher than the other treatments. For rooting, the effect of basal media (MS, 1/2 MS (macroelements half) and 1/2 MS (macro and micro elements half)) and different auxins including IBA, NAA and IAA, solely or in combination with each other was investigated. The results showed that the highest percentage of rooting was due to medium 1/2 MS containing (macroelements half) 1 mg L -1 IBA resulted in 100% rooting. Due to low quality of roots obtained, and also the role of Fe-EDDHA and thiamine in rooting improvement, an experiment about the effects of above-mentioned elements was carried out. The results indicated that thiamine and Fe-EDDHA at concentrations of 1.6 and 150 mg L -1 resulted in the best rooting. Finally, in acclimation phase, more than %95 survival was achieved. -1 respectively
  • ItemOpen Access
    Relative susceptibility of selected apple cultivars to alternate bearing
    (Uludağ Üniversitesi, 2013-07-22) Atay, Ayşe Nilgün; Koyuncu, Fatma; Atay, Ersin
    The negative effects of alternate bearing on yield and fruit quality are a well-known. The objective of this study was to determine the sensitivity of 17 apple scions grafted onto MM106 rootstocks to alternate bearing. Quantitative evaluation of alternation was used modified alternate bearing index (MABI) which take into the flower production of the cultivars to define the alternate bearing. The lowest MABI was 0.20 in ‘Braeburn’, while ‘Golden Reinders’ and ‘Kaşel41’ had highest values of MABI as 0.78. Based on MABI values of 3 consecutive year of this research, cultivars were classified into four relative susceptibility groups: not susceptible: ‘Braeburn’ and ‘Jerseymac’; medium alternance: ‘Topaz’, ‘Granny Smith’, ‘Mondial Gala’, ‘Jonagold’, ‘Starkrimson Delicious’ and ‘Clear Red’; susceptible to alternance: ‘Starkspur Golden Delicious’, ‘Fuji’, ‘Arlet’, ‘Redchief Delicious’, ‘Rajka’ and ‘Golden Delicious’; and high alternance: ‘Kaşel 37’, ‘Golden Reinders’ and ‘Kaşel 41’. The fact that cultivar had the greatest impact on alternate bearing. Differences among cultivars in irregular cropping cycles can be used by growers and researchers to mitigate this phenomenon or improve new practices based on cultivar susceptibility.
  • ItemOpen Access
    Effect of silicon on some physiological properties of maize (zea mays) under salt stress
    (Uludağ Üniversitesi, 2013-07-22) Rohanipoor, Ali; Norouzi, Mehdi; Moozzi, Abdolamir; Hassibi, Payman
    Salt stress is an abiotic stress that can effect the plant growth and physiological and biochemical activities such as photosynthesis activity and chlorophyll content. This study was conducted in order to evaluate effects of silicon (Si) on some physiological properties of maize (Zea mays) under salt stress. Selected seedlings were transplanted to plastic pots contained sterilized and non-saline sandy soil that continuously aerated full-strength Hoagland nutrient solution. The Si was added at four levels (0, 2, 4 and 6 mmol.L-1 ) from source potassium silicate (K2SiO3) and salt stress was applied at four levels (0, 3, 6 and 9 dS.m-1 ) from source sodium chloride (NaCl). A factorial experiment in a completely randomized design (CRD) with sixteen treatments and three replications was applied. Results indicated that salinity significantly decreased the fresh and dry weights of shoot and root, stem length, leaf area, chlorophyll content and relative water content (RWC) of maize plant and application of Si significantly increased them. Remarkable decrease observed in treatments with EC > 3 dS.m-1 , while, gradual increasing of Si increased physiological properties of maize. Therefore, proper Si nutrition can increase salt resistance in maize plant.
  • ItemOpen Access
    Biochemical alterations induced by sublethal cyanide exposure in common carp (Cyprinus carpio)
    (Uludağ Üniversitesi, 2013-06-25) Sadati, Fahimeh; Shahsavani, Davar; Baghshani, Hasan
    The present study was designed to study the effects of sublethal cyanide exposure on some blood biochemical parameters in common carp. Three groups of fish (25 in each group) were treated with different concentrations of 0 (control), 0.1 or 0.2 mg/L potassium cyanide (KCN) for 2 weeks. At the end of each exposure, blood samples were collected and serum biochemical parameters were measured using validated methods. Cyanide exposure at both concentrations caused significant (P<0.05) elevation of aspartate aminotransferase and lactate dehydrogenase activities and creatinine concentration as compared to control group. Indeed, alanine aminotransferase, alkaline phosphatase and glucose levels in the carp treated with 0.2 mg/L KCN were significantly higher than those levels from controls. On the other hand, other measured biochemical parameters including gamma-glutamyl transferase, triglyceride, cholesterol, total protein, and albumin did not change significantly following cyanide exposure. The observed alterations in some measured serum biochemical parameters would reflect tissue damages, especially in liver and kidney, due to chronic cyanide intoxication in common carp and may be used for better understanding the pathophysiology of this toxicity and as an aid in the diagnosis of cyanide poisoning in fish species.
  • ItemOpen Access
    In vitro culture of gisela 6 semi-dwarf rootstock
    (Uludağ Üniversitesi, 2013-06-03) Moghaddam, Rahim Nazary; Yadollahi, Abbas; Moeini, Ahmad; Sepahvand, Sadegh
    This research was carried out to study micropropagation of Gisela 6 rootstock in order to achieve suitable media for producing 5 thousand plants. Explants were cultured in MS and WPM media containing 2 types of cytokinins BAP and TDZ at different concentrations. The results showed that the number of shoots in MS medium containing 1.2 mg L-1 BAP was higher than the other treatments. For rooting, the effect of basal media (MS, 1/2 MS (macroelements half) and 1/2 MS (macro and micro elements half)) and different auxins including IBA, NAA and IAA, solely or in combination with each other was investigated. The results showed that the highest percentage of rooting was due to medium 1/2 MS containing (macroelements half) 1 mg L-1 IBA resulted in 100% rooting. Due to low quality of roots obtained, and also the role of Fe-EDDHA and thiamine in rooting improvement, an experiment about the effects of above-mentioned elements was carried out. The results indicated that thiamine and Fe-EDDHA at concentrations of 1.6 and 150 mg L-1 respectively resulted in the best rooting. Finally, in acclimation phase, more than %95 survival was achieved.
  • ItemOpen Access
    Evaluation of different techniques for breaking seed dormancy of heliotropium europaeum l. (Boraginaceae)
    (Uludağ Üniversitesi, 2013-08-29) Aliloo, All Asghar; Darabinejad, Shabnam
    Prediction of germination and emergence of weeds plays a key role in weed management strategies. For this reason, the seed dormancy status of Heliotropium europaeum (Boraginaceae) was investigated in North West of Iran. The dormancy breaking treatments were after-ripening, gibberellic acid, hot water, KNO3, cold stratification, and washing. Seeds were strongly dormant at maturity and maintained high levels of dormancy for long periods of time. However, after 12 months storage at dry conditions, germination percentage increased to 37%. Washing and hot water treatments did not alter seed dormancy behavior, while the influence of gibberellic acid, KNO3 and cold stratification was significant. The highest value for germination percentage obtained by cold stratification increased the value up to 90%. Results of seedling growth showed that compared to other treatments, the seeds treated by KNO3 produced longer shoots and vigorous seedlings. The high vigor index was also recorded for cold stratification. It is concluded that the seeds of Heliotrope had a high primary dormancy and among treatments, the cold stratification was a more effective method in breaking seed dormancy .