Hazır ve eski peteklerin bakteriyolojik ve mikolojik yönden incelenmesi
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Date
2003
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Uludağ Üniversitesi
Abstract
Bu çalışmada Bursa ve Yalova yörelerinde yavru çürüklüğü şüpheli 24 farklı arılıktan alınan eski petek ile ticari firmalar tarafından üretilen 11 değişik marka hazır petekte insan ve arı sağlığına zararlı bakteriyel ve fungal mantar etkenlerin araştırılması amaçlanmıştır. Eski peteklerin tamamında şüpheli gözeneklerden alınan örnekler ve ticari temel peteklerin yıkama solüsyonları, Amerikan yavru çürüğü ve Avrupa yavru çürüğü etkenleri ve olası diğer etkenlerin izolasyonu açısından uygun ortamlarda inkübe edilmiştir. İnkübasyon sonrası üreyen farklı morfolojideki mikroorganizmalar gram boyama yöntemi ile incelendikten sonra CRYSTAL Sistemi (Becton Dickinson, Aalst, Belgium) ile teşhis edilmiştir. Eski peteklerin tamamında bir veya birden fazla bakteriyel ve fungal etkene rastlanırken, ticari peteklerin 6’sında (%54,5) etken izolesyonu yapılmış 5’inde (%45,5) herhangi bir etken tespit edilmemiştir. Etken izole edilen ticari temel peteklerin 5’inin (%83,3) bakteriler birinin (%16,7) fungal etkenler ile kontamine olduğu tespit edilmiştir. Eski peteklerde 14 bakteri türü (Bacillus brevis, Bacillus cereus, Bacillus megatarium, Bacillus sphaericus, Bacillus subtilis, Corynebacterium bovis, Corynebacterium jekium, Enterecoccus faecalis, Micrococcus luteus, Proteus mirabilis, Staphylococcus epidermidis, Staphylococcus vitulus, Staphylococcus warneri, Streptococcus vestibularis) ve 4 mantar cins ve türü (Aspergillus fumigatus, Candida spp., Cladosporium corrioni, Penicillum spp.) teşhis edilmiştir. Bu etkenlerden C. jekium 9 örnekte rastlanmıştır. Ticari temel peteklerde 4 bakteri türü (Bacillus pumilus, Bacillus subtilis, Staphylococcus auricularis, Staphylococcus epidermidis) ve bir fungus cinsi (Candida spp.) saptanmış ve B. subtilis 2 örnekte tespit edilmiştir. İncelenen tüm örneklerde Amerikan yavru çürüğü etkeni P.l alvei ve Avrupa yavru çürüğü etkeni M. Pluton saptanmamıştır. Sonuçlar insan ve arı sağlığı yönünden değerlendirilmiştir.
The goal of this study was to isolate bacteria and fungi sp. from 24 combs taken from different apiaries, and 11 commercial foundations and their effects on honeybee and human health in Bursa-Yalova provinces. Washing solutions, taken from suspected comb cells and commercial foundations for AFB, EFB causative agents Paenibacillus alvei and Melissococcus pluton and other agents, were incubated. After incubation, growing colonies with different morphologies were gram stained and diagnosed by CRYSTAL System (Becton Dickinson, Aalst, Belgium). All of the combs were contaminated with bacteria and fungi, only 6 (54.5%) of commercial foundations were contaminated, 5 of them, (45.5%) were not contaminated. From commercial foundations, 5 (83.3%) were with bacteria, and 1 (16.7%) were contaminated with fungi, 14 bacteria sp. (Bacillus brevis, Bacillus cereus, Bacillus megatarium, Bacillus sphaericus, Bacillus subtilis, Corynebacterium bovis, Corynebacterium jekium, Enterecoccus faecalis, Micrococcus luteus, Proteus mirabilis, Staphylococcus epidermidis, Staphylococcus vitulus, Staphylococcus warneri, Streptococcus vestibularis) and 4 fungi sp. (Aspergillus fumigatus, Candida spp., Cladosporium corrioni, Penicillum spp.) isolated from combs and diagnosed. C. jekium was found in 9 samples, 4 bacteria sp. (Bacillus pumilus, Bacillus subtilis, Staphylococcus auricularis, Staphylococcus epidermidis) and 1 fungus genus (Candida spp.) was detected, B. subtilis was found in two samples. AFB causative agent P.l alvei and EFB M.pluton were not detected in any sample examined. The results were evaluated for honeybee and human health.
The goal of this study was to isolate bacteria and fungi sp. from 24 combs taken from different apiaries, and 11 commercial foundations and their effects on honeybee and human health in Bursa-Yalova provinces. Washing solutions, taken from suspected comb cells and commercial foundations for AFB, EFB causative agents Paenibacillus alvei and Melissococcus pluton and other agents, were incubated. After incubation, growing colonies with different morphologies were gram stained and diagnosed by CRYSTAL System (Becton Dickinson, Aalst, Belgium). All of the combs were contaminated with bacteria and fungi, only 6 (54.5%) of commercial foundations were contaminated, 5 of them, (45.5%) were not contaminated. From commercial foundations, 5 (83.3%) were with bacteria, and 1 (16.7%) were contaminated with fungi, 14 bacteria sp. (Bacillus brevis, Bacillus cereus, Bacillus megatarium, Bacillus sphaericus, Bacillus subtilis, Corynebacterium bovis, Corynebacterium jekium, Enterecoccus faecalis, Micrococcus luteus, Proteus mirabilis, Staphylococcus epidermidis, Staphylococcus vitulus, Staphylococcus warneri, Streptococcus vestibularis) and 4 fungi sp. (Aspergillus fumigatus, Candida spp., Cladosporium corrioni, Penicillum spp.) isolated from combs and diagnosed. C. jekium was found in 9 samples, 4 bacteria sp. (Bacillus pumilus, Bacillus subtilis, Staphylococcus auricularis, Staphylococcus epidermidis) and 1 fungus genus (Candida spp.) was detected, B. subtilis was found in two samples. AFB causative agent P.l alvei and EFB M.pluton were not detected in any sample examined. The results were evaluated for honeybee and human health.
Description
Keywords
Hazır petek, Eski petek, Bakteri, Mantar, Comb, Foundation, Bacteria, Fungi
Citation
Özakın, C. vd. (2003). ''Hazır ve eski peteklerin bakteriyolojik ve mikolojik yönden incelenmesi''. Uludağ Arıcılık Dergisi, 3(1), 27-30.