Azerbaycan propolisinde faydalı biyolojik aktiviteye sahip fenolik bileşiklerin nitel ve nicel olarak incelenmesi
Loading...
Date
2019-09-02
Authors
Sarıyev, Rashad
Journal Title
Journal ISSN
Volume Title
Publisher
Bursa Uludağ Üniversitesi
Abstract
Propolis, bal arılarının bitki ve ağaçların sürgün, çatlak gibi kısımlarında bulunan reçine ve salgıları toplayıp oluşturdukları bir arı ürünüdür. Çok eskilerden beri insanlar tarafından bilinen propolisin zengin içeriği ve biyolojik özelliği sebebiyle günümüzde apiterapi, sağlıklı beslenme, gıda ve biyokozmetik alanında kullanımı yaygınlaşmıştır. Fenolik bileşiklerin propolisin ana kimyasal bileşenleri olduğu ve propolise biyolojik özellikler kazandırdığı bilinmektedir. Ayrıca propolisin içeriğindeki fenolik bileşiklerin sayı ve miktarları, toplandıkları bölgelere göre değişim gösterebilmektedir. Bu çalışmanın amacı Azerbaycan propolislerinin içerdiği faydalı biyolojik özelikleri bilinen fenolik bileşiklerin sayı ve miktarlarını belirleyerek Azerbaycan propolisinin kalitesi ile ilgili bilgi sahibi olmaktır. Bu amaçla 2018 Eylül ayında Azerbaycan’ın 5 ilinde bulunan 14 farklı arılıktan propolisler kazınarak toplandı. Propolisteki 17 farklı fenolik bileşiğin (gallik asit, epigallokateşin gallat, kafeik asit, ferulik asit, isoferulik asit, metilşiringat, 3,4-dimetoksisinamik asit, kuersetin, sinnamik asit, naringenin, apigenin, kaemferol, krisin, pinosembrin, galangin, kafeik asit fenetil ester ve kalkon) analizi nicel ve nitel olarak HPLC-DAD sistemi kullanılarak yapıldı. Analiz sonucunda, Azerbaycan propolislerinde toplamda hedeflenen 17 fenolik bileşik ölçülebilir düzeylerde tespit edildi. Tespit edilen toplam 17 farklı fenolik bileşikten epigallokateşin gallat, kafeik asit, ferulik asit ve isoferulik asit 14 numunenin tamamında tespit edildi. Sonuç olarak, bu çalışmadaki Azerbaycan propolislerinin fenolik bileşik içeriğinin Avrupa, Asya ve Kuzey Amerika propolislerinin yapısıyla benzerlik gösterdiği ve miktarları açısından değerlendirildiğinde analizi yapılmış ve belirli bir kaliteye sahip diğer ülkelerin propolisleriyle benzer kaliteye sahip olduğu tespit edilmiştir.
Propolis is a bee product that honey bees collect and form resins and secretions found in the shoots and cracks of plants and trees. Propolis has been known to humans since ancient times, and due to its rich content and biological properties, it has become widespread in apitherapy, healthy nutrition, food and biocosmetics. It is known that phenolic compounds are the main chemical components of propolis and give propolis biological properties. In addition, the number and amount of phenolic compounds in propolis may vary depending on the regions where they are collected. The aim of this study is to determine the number and amount of phenolic compounds which have beneficial biological properties are known in the content of Azerbaijani propolis and thus to have knowledge about the quality of Azerbaijan propolis. For this purpose, scraped propolis samples collected from 14 different apiaries in 5 provinces in September 2018 of Azerbaijan. 17 different phenolic compounds (gallic acid, epigallocatechin gallate, caffeic acid, ferulic acid, isoferulic acid, methylchiringate, 3,4-dimethoxycinamic acid, quercetin, cinnamic acid, naringenin, apigenin, kaemferol, chrisin, pinosembrin, galangin, caffeic acid phenethyl ester and chalcone) analysis were performed quantitatively and qualitatively using HPLC-DAD system. As a result of the analysis, a total of 17 targeted phenolic compounds were detected at measurable levels in Azerbaijani propolis. Epigallocatechin gallate, caffeic acid, ferulic acid and isoferulic acid from a total of 17 different phenolic compounds were detected in all 14 samples. As a result, the phenolic compound content of Azerbaijan propolis in this study is similar to the structure of European, Asian and North American propolis and confirmed has a certain quality when evaluated in terms of their amounts with analyzed results of other countries.
Propolis is a bee product that honey bees collect and form resins and secretions found in the shoots and cracks of plants and trees. Propolis has been known to humans since ancient times, and due to its rich content and biological properties, it has become widespread in apitherapy, healthy nutrition, food and biocosmetics. It is known that phenolic compounds are the main chemical components of propolis and give propolis biological properties. In addition, the number and amount of phenolic compounds in propolis may vary depending on the regions where they are collected. The aim of this study is to determine the number and amount of phenolic compounds which have beneficial biological properties are known in the content of Azerbaijani propolis and thus to have knowledge about the quality of Azerbaijan propolis. For this purpose, scraped propolis samples collected from 14 different apiaries in 5 provinces in September 2018 of Azerbaijan. 17 different phenolic compounds (gallic acid, epigallocatechin gallate, caffeic acid, ferulic acid, isoferulic acid, methylchiringate, 3,4-dimethoxycinamic acid, quercetin, cinnamic acid, naringenin, apigenin, kaemferol, chrisin, pinosembrin, galangin, caffeic acid phenethyl ester and chalcone) analysis were performed quantitatively and qualitatively using HPLC-DAD system. As a result of the analysis, a total of 17 targeted phenolic compounds were detected at measurable levels in Azerbaijani propolis. Epigallocatechin gallate, caffeic acid, ferulic acid and isoferulic acid from a total of 17 different phenolic compounds were detected in all 14 samples. As a result, the phenolic compound content of Azerbaijan propolis in this study is similar to the structure of European, Asian and North American propolis and confirmed has a certain quality when evaluated in terms of their amounts with analyzed results of other countries.
Description
Keywords
Fenolik bileşikler, Propolis, Azerbaycan, Azerbaijan, HPLC-DAD, Phenolic compounds
Citation
Sarıyev, R. (2019). Azerbaycan propolisinde faydalı biyolojik aktiviteye sahip fenolik bileşiklerin nitel ve nicel olarak incelenmesi. Yayınlanmamış yüksek lisans tezi. Bursa Uludağ Üniversitesi Sağlık Bilimleri Enstitüsü.