Detection of shiga-toxin producing e.coli (stec), enteropathogenic e.coli (epec) and enterotoxigenic e.coli (etec) from animals by m-pcr
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Date
2019-11-10
Authors
Khider, Mohammed
Journal Title
Journal ISSN
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Publisher
Bursa Uludağ Üniversitesi
Abstract
This research investigated the presence of virulence genes encoding F41, K99, eae, Stx1, Stx2 and STa and the antimicrobial resistance of animal Escherichia coli (E. coli) isolates. Clinical isolates (n:233) were evaluated from fecal samples of cattle, sheep, goats, horses, cats and dogs collected between the years of 2010 to 2015 from Turkey. Enterohaemorrahic E.coli (EHEC) O157:H7 was detected by using cefixime tellurite sorbitol MacConkey agar (CT-SMAC) and Wellcollex E. coli (Remel®). The Kirby-Bauer disc diffusion test was performed to detect the resistance pattern of the isolates to ampicillin, Amoxycilin/clavulanic acid, enrofloxacin, ceftiofur, trimethoprim/sulfamethoxazole and tetracycline. The results showed that 40% of the ruminant isolates were identified as Shiga-toxin producing Escherichia coli (STEC). Enterotoxigenic E. coli (ETEC) was detected in samples from cattle (0.9%) and sheep (12%). Enteropathogenic E.coli (EPEC) was detected in samples from cattle (0.9%) and dogs (11.4%). EHEC O157:H7 was not detected any of the isolate. Among all E.coli isolates that carried at least one virulence gene, 8 (19%) were resistant to more than three antimicrobials, 7 (16.7%) were resistant to at least one antimicrobial and 27 (64.3%) were susceptible to all antimicrobials.
Description
Keywords
Antimicrobial susceptibility, E. coli, STEC, PCR, EPEC, ETEC
Citation
Büyükcangaz, E. vd. (2019). "Detection of shiga-toxin producing e.coli (stec), enteropathogenic e.coli (epec) and enterotoxigenic e.coli (etec) from animals by m-pcr". Bursa Uludağ Üniversitesi Veteriner Fakültesi, 38(2), 88-94.