Burdur İl’inde toplanan silajlarda mikotoksin varlığının ve düzeylerinin araştırılması
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Date
2010-07-20
Authors
Şahindokuyucu, Fatma
Mor, Firdevs
Oğuz, M. Numan
Oğuz, Karakaş Fatma
Journal Title
Journal ISSN
Volume Title
Publisher
Uludağ Üniversitesi
Abstract
Bu çalışmada, Burdur İl’inde Aralık 2006-Mayıs 2007 tarihleri arasında toplanan 60 mısır silajı örneğinin total aflatoksin, okratoksin A, T-2 toksin, deoksinivalenol, zearalenon ve fumonisin yönünden analizlerinin yapılması amaçlanmıştır. Silaj örnekleri, kış ve ilkbahar mevsimlerinde toplanmıştır. Örnekler, Enzyme Linked Immunosorbent Assay (ELISA) yöntemi ile analiz edilmiştir. Silaj örneklerinin total aflatoksin, okratoksin A, T2 toksin, deoksinivalenol, zearalenon ve fumonisin ile kirlenme sıklığı sırasıyla, 18 (% 30), 8 (%13,3), 21 (% 35), 23 (% 38,3), 23 (% 38,3), 1 (% 1,7) olarak bulunmuştur. Total aflatoksin, okratoksin A, T-2 toksin, deoksinivalenol, zearalenon ve fumonisin düzeyleri ise, sırasıyla 4.33-19.92 µg/kg, 1.76-3.26 µg/kg, 3.85-15.40 µg/kg, 24.20-100.30 µg/kg, 2.84-40.64 µg/kg, 2690 µg/kg olarak tespit edilmiştir. Silaj örneklerinin en fazla deoksinivalenol ve zearalenon ile kirlendiği tespit edilmiştir. Silaj örneklerinde kış ve ilkbahar mevsimlerinde total aflatoksin, okratoksin A, deoksinivalenol, zearalenon ve fumonisin düzeyleri arasında istatistiksel olarak fark bulunmazken, T-2 toksin düzeyleri ilkbahar mevsiminde kışa göre önemli (p<0.05) düzeyde yüksek bulunmuştur Sonuç olarak, silaj örneklerinde tespit edilen mikotoksin düzeylerinin, hayvan sağlığı için tehlike oluşturacak düzeyde olmadığı kanısına varılmıştır.
The aim of this research was to determine total aflatoxin, ochratoxin, T-2 toxin, deoxynivalenol, zearalenone and fumonisin levels of 60 corn silage samples collected in Burdur province between December 2006-May 2007. The silage samples were collected in winter and spring. The samples were analysed by Enzyme Linked Immuno Sorbent Assay (ELISA) method. The incidence of total aflatoxin, ochratoxin A, T-2 toxin, deoxynivalenol, zearalenone and fumonisin in the silage samples were found as 18 (30 %), 8 (13,3 %), 21 (35 %), 23 (38,3 %), 23 (38,3 %) and 1 (1,7 %), respectively. The total aflatoxin, ochratoxin A, T-2 toxin, deoxynivalenol, zearalenone and fumonisin levels were detected as 4.33-19.92 µg/kg, 1.76-3.26 µg/kg, 3.85-15.40 µg/kg, 24.20-100.30 µg/kg, 2.84-40.64 µg/kg, 2690 µg/kg, respectively. The highest level of mycotoxins detected in the silage samples were deoxynivalenol and zearalenone. Statistical evaluations showed that mean contamination levels of T-2 toxin in spring silage samples were significantly higher (p<0.05) than those of winter, while the results showed that there were no statistical differences between total aflatoxin, ochratoxin A, deoxynivalenol, zearalenone and fumonisin levels of spring and winter silage samples. It was concluded that the mycotoxin levels detected in the silage samples could not be considered as a risk for animal health.
The aim of this research was to determine total aflatoxin, ochratoxin, T-2 toxin, deoxynivalenol, zearalenone and fumonisin levels of 60 corn silage samples collected in Burdur province between December 2006-May 2007. The silage samples were collected in winter and spring. The samples were analysed by Enzyme Linked Immuno Sorbent Assay (ELISA) method. The incidence of total aflatoxin, ochratoxin A, T-2 toxin, deoxynivalenol, zearalenone and fumonisin in the silage samples were found as 18 (30 %), 8 (13,3 %), 21 (35 %), 23 (38,3 %), 23 (38,3 %) and 1 (1,7 %), respectively. The total aflatoxin, ochratoxin A, T-2 toxin, deoxynivalenol, zearalenone and fumonisin levels were detected as 4.33-19.92 µg/kg, 1.76-3.26 µg/kg, 3.85-15.40 µg/kg, 24.20-100.30 µg/kg, 2.84-40.64 µg/kg, 2690 µg/kg, respectively. The highest level of mycotoxins detected in the silage samples were deoxynivalenol and zearalenone. Statistical evaluations showed that mean contamination levels of T-2 toxin in spring silage samples were significantly higher (p<0.05) than those of winter, while the results showed that there were no statistical differences between total aflatoxin, ochratoxin A, deoxynivalenol, zearalenone and fumonisin levels of spring and winter silage samples. It was concluded that the mycotoxin levels detected in the silage samples could not be considered as a risk for animal health.
Description
Keywords
Mısır silajı, Corn silage, Mikotoksin, Mycotoxin
Citation
Dokuyucu, Ş. vd. (2010). "Burdur İl’inde toplanan silajlarda mikotoksin varlığının ve düzeylerinin araştırılması". Uludağ Üniversitesi Veteriner Fakültesi Dergisi, 29(1), 49-54.