İnek ve düvelerde luteal aktivitenin ovsynch protokolüne etkisi
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Date
2009-04-03
Authors
Kırbaş, Mesut
Çoyan, Kenan
Bülbül, Bülent
Ataman, Mehmet Bozkurt
Köse, Mehmet
Akman, Orhan
Dursun, Şükrü
Journal Title
Journal ISSN
Volume Title
Publisher
Uludağ Üniversitesi
Abstract
İneklerde östrüs senkronizasyonu için kullanılan ovsynch protokolünün siklusun luteal veya folliküler dönemlerinde başlamasının gebelik oranlarına etkisinin araştırılması amacıyla, 22 inek ve 58 düve, uygulamanın başlangıcındaki serum progesteron düzeylerine göre iki gruba ayrıldı. Bütün hayvanlar 0. gün 50 µg lesirelin acetate ve bundan 7 gün sonra 0.150 mg d-kloprostenol enjeksiyonu ile senkronize edildi. GnRH enjeksiyonu sırasında serum progesterone seviyesi <1 ng/ml olan hayvanlar luteal olarak aktif olmayan dönemde (Grup I, n=34, 7 inek ve 27 düve) kabul edilirken, ≥1 ng/ml olanlar luteal olarak aktif (Grup II, n=46, 15 inek ve 31 düve) kabul edildi. Bütün hayvanlara, PGF2α uygulamasından 48 saat sonra ikinci GnRH enjekte edildi ve hayvanlar bundan 16 saat sonra sabit zamanlı tohumlandı. Gebelik muayeneleri tohumlama sonrası 28. gün ultrason ile 7.5 MHz rektal prob kullanılarak yapıldı. Đneklerde ve düvelerde gebelik oranları sırasıyla Grup I’de %42.9 ve %55.6 ve Grup II’de ise %33.3 ve %41.9 olarak bulundu. Gruplarda tespit edilen gebelik oranları arasındaki fark istatistiki açıdan önemli bulunmadı. Sonuç olarak, inek ve düvelerde östrüs senkronizasyonu amacıyla kullanılan ovsynch protokolünün başlangıcındaki luteal aktivitenin gebelik oranını etkilemediği kanısına varıldı.
To compare the effect of ovsynch protocol beginning in luteal or follicular phases on pregnancy rates in cows and heifers, a total of 22 cows and 58 heifers were allocated into two groups according to their serum progesterone levels at the beginning of the ovsynch protocol. All animals were synchronized with i.m. injection of 50 µg lesirelin acetate on d 0 and 0.150 mg d-kloprostenol 7 d later. At the time of GnRH injection, animals with <1 ng/ml serum progesterone concentration were accepted as luteally not active (Group I, n=34, 7 cows and 27 heifers) while others with ≥1 ng/ml serum progesterone concentration were accepted as luteally active (Group II, n=46, 15 cows and 31 heifers). Second dose of GnRH was injected 48 h after PGF2α injections and fixed-time AI were performed 16 h after the second GnRH in all animals. Pregnancy diagnoses were performed on d 28 by ultrasonography with 7.5 MHz transrectal probe. There was no difference between cows and heifers in pregnancy rates. Pregnancy rates in cows and heifers were 42.9% and 55.6% in group I and 33.3% and 41.9% in group II. The differences between groups were not significant. It is concluded that, luteal activity at the beginning of ovsynch protocol for oestrus synchronization did not make any difference on pregnancy rates in cows and heifers
To compare the effect of ovsynch protocol beginning in luteal or follicular phases on pregnancy rates in cows and heifers, a total of 22 cows and 58 heifers were allocated into two groups according to their serum progesterone levels at the beginning of the ovsynch protocol. All animals were synchronized with i.m. injection of 50 µg lesirelin acetate on d 0 and 0.150 mg d-kloprostenol 7 d later. At the time of GnRH injection, animals with <1 ng/ml serum progesterone concentration were accepted as luteally not active (Group I, n=34, 7 cows and 27 heifers) while others with ≥1 ng/ml serum progesterone concentration were accepted as luteally active (Group II, n=46, 15 cows and 31 heifers). Second dose of GnRH was injected 48 h after PGF2α injections and fixed-time AI were performed 16 h after the second GnRH in all animals. Pregnancy diagnoses were performed on d 28 by ultrasonography with 7.5 MHz transrectal probe. There was no difference between cows and heifers in pregnancy rates. Pregnancy rates in cows and heifers were 42.9% and 55.6% in group I and 33.3% and 41.9% in group II. The differences between groups were not significant. It is concluded that, luteal activity at the beginning of ovsynch protocol for oestrus synchronization did not make any difference on pregnancy rates in cows and heifers
Description
Keywords
Düve, Cow, İnek, Luteal aktivite, Progesteron, Heifer, Luteal activity, Ovsynch, Progesterone
Citation
Kırbaş, M. vd. (2009). "İnek ve düvelerde luteal aktivitenin ovsynch protokolüne etkisi". Uludağ Üniversitesi Veteriner Fakültesi Dergisi, 27 (1-2), 47-52.