Pekin ördeklerinde büyüme hormonu geni polimorfizminin incelenmesi
Loading...
Files
Date
2019-10-18
Authors
Eriş, Candan
Journal Title
Journal ISSN
Volume Title
Publisher
Bursa Uludağ Üniversitesi
Abstract
Bu çalışmanın temel amacı, Pekin ördeklerinde büyüme hormonu (BH) geninin 2’inci, 3’üncü ve 4’üncü intronlarındaki polimorfizmleri araştırmaktır. Bu amaçla her iki cinsiyetten yetişkin Pekin ördeklerinden 5-10 ml kan alınmıştır. BH geninin 2’inci, 3’üncü ve 4’üncü intronlarındaki genetik polimorfizm, üç primer çifti ile PCR-RFLP yönteminde BsmF1 enzimi kullanılarak belirlenmiştir. Yapılan araştırmaların sonucunda sadece 2’inci intron bölgesinden çoğaltılmış bölgeler polimorfizm göstermişlerdir. 3’üncü ve 4’üncü intronlarda ise genetik varyasyon gözlenmemiştir. 2’inci intron bölgesinde üç genotip (TT, CT ve CC) gözlenmiş ve T ve C allellerinin frekansları sırasıyla 0,7521 ve 0,2479 olarak hesaplanmıştır. Çalışılan populasyonun Hardy- Weinberg dengesinde olduğu gözlenmiştir.
The main objective of the study was to investigate the polymorphisms in intron 2, 3 and 4 of the growth hormone (GH) gene of Pekin ducks. For this purpose, in the both sex 5- 10 ml blood samples were obtained from of adult Pekin ducks. Three primers were used to determined genetic polymorphisms of intron 2, 3 and 4 of the GH gene with PCRRFLP methods using BsmF1. As a results of studies only the products amplified from intron 2 showed polymorphisms. No genetic variation was found in intron 3 and intron 4. Three genotypes (TT, CT and CC) were observed in the intron 2 region. The gene frequencies of T and C alleles were calculated to be 0.7521 and 0.2479, respectively. The studied population was observed to be in Hardy-Weinberg equilibrium.
The main objective of the study was to investigate the polymorphisms in intron 2, 3 and 4 of the growth hormone (GH) gene of Pekin ducks. For this purpose, in the both sex 5- 10 ml blood samples were obtained from of adult Pekin ducks. Three primers were used to determined genetic polymorphisms of intron 2, 3 and 4 of the GH gene with PCRRFLP methods using BsmF1. As a results of studies only the products amplified from intron 2 showed polymorphisms. No genetic variation was found in intron 3 and intron 4. Three genotypes (TT, CT and CC) were observed in the intron 2 region. The gene frequencies of T and C alleles were calculated to be 0.7521 and 0.2479, respectively. The studied population was observed to be in Hardy-Weinberg equilibrium.
Description
Keywords
Growth Hormone (GH) gene, Büyüme Hormonu (BH) geni, PCR-RFLP, Polymorphisms, Pekin duck, PCR-RFLP, Polimorfizm, Pekin ördeği
Citation
Candan, E. (2019). Pekin ördeklerinde büyüme hormonu geni polimorfizminin incelenmesi. Yayınlanmamış yüksek lisans tezi. Bursa Uludağ Üniversitesi Fen Bilimleri Enstitüsü.