Browsing by Author "Toker, M. Berk"

Now showing 1 - 8 of 8

Administration time of misoprostol affects fertility rate in artificially inseminated Kivircik ewes with frozen-thawed ram semen
(Brazilian Coll Animal Reproduction, 2018-05-15) Demir, Kamber; Üstüner, Hakan; Üstüner, Burcu; Toker, M. Berk; Alçay, Selim; Sağırkaya, Hakan; Nur, Zekeriya; Uludağ Üniversitesi/Veteriner Fakültesi/Zootekni Anabilim Dalı.; Uludağ Üniversitesi/Veteriner Fakültesi/Üreme ve Suni Tohumlama Anabilim Dalı.; 0000-0002-1438-221X; 0000-0003-4033-9749; 0000-0002-4561-6189; 0000-0002-4341-5842; AAH-2635-2021; A-2794-2014; D-2411-2019; AAG-9127-2021; AAH-8821-2021; AAG-7238-2021; AAH-8751-2019; 16065222700; 18937724600; 56480349200; 56099810300; 6602400461; 6508060684
The aim of this study was to determine the effects of the administration time of misoprostol (11 h (Miso11) and 6 h (Miso6) before artificial insemination) on fertility rates in Kivircik ewes (control: n = 41, Miso11: n = 32 and Miso6: n = 33) during breeding season. Artificial insemination (AI) was performed 48 h after sponge removal using frozen-thawed semen (150 million sperm per dose in 0.25 ml straws). Estrus synchronization parameters (onset and duration) and lambing rate were evaluated. No significant difference was observed among groups for the estrus onset and duration hours (P > 0.05). The lambing rates in the control, Miso11 and Miso6 groups were 39.0, 62.5 and 54.5%, respectively. There were significant differences among the control, Miso11 and Miso6 groups according to lambing rates (P < 0.05). In conclusion, misoprostol treatment significantly improved fertility in ewes when using frozen-thawed semen in AI. Administration of misoprostol 11 h before AI resulted in a higher lambing rate than that at 6 h before AI; therefore, treatment of misoprostol 11 h before AI can effectively be used.
Cryopreservation of ram semen with antioxidant supplemented soybean lecithin-based extenders and impacts on incubation resilience
(Elsevier, 2016-06) Toker, M. Berk; Alçay, Selim; Gökçe, Elif; Üstüner, Burcu; Uludağ Üniversitesi/Veteriner Fakültesi/Üreme ve Suni Tohumlama Anabilim Dalı.; 0000-0002-7678-3289; 0000-0003-4033-9749; AAG-7238-2021; ABA-6294-2020; 56480349200; 56099810300; 56779799700; 18937724600
The scope of this study was investigation the affects of various antioxidants on 1% soybean lecithin-based semen extenders for ram semen cryopreservation. Ejaculates, collected via electrically stimulated ejaculation, that have a thick consistency, rapid wave motion (3-5 on a 0-5 scale) and >75% initial motility were pooled. The pooled samples were split into four equal aliquots as 5 mM Methionine, 5 mM Cysteamine, 1 mM Cysteine and a sample of antioxidant-free control group. Each sample group was diluted to a ratio of 1/5 (semen/extender, v/v) as final concentration and two step dilution method was used for cryopreservation. Extender groups were assessed for sperm motility, plasma membrane functional integrity using hypoosmotic swelling test (HOST), damaged acrosome using FITC-Pisum sativum agglutinin (PSA-FITC) and DNA integrity using terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL). Semen samples also incubated for 6 h in humidified air with 5% CO2 at 39 degrees C to evaluate post-thaw incubation resilience of semen characteristics. The results showed that freezing and thawing procedures had negative effects on motility (P < 0.05), plasma membrane integrity (P < 0.05) and acrosomal integrity (P < 0.05). After 6 h of incubation time, the Cysteine supplemented extender group yielded significantly higher results than other extender groups in terms of spermatological parameters. Furthermore MDA levels in the antioxidant groups were lower than control group (P < 0.05). Nevertheless, there were no significant differences among antioxidant groups.
Does isoxsuprine hcl facilitate the passage of the cervix in sheep?: A case series
(Sivar-soc Italiana Veterinari Animali Reddito, 2023-04-01) Önder, N. Tekin; Gökdemir, Taygun; Kılıç, Muhammet Can; Şahin, Oğuzhan; Yıldız, Savaş; Öztürkler, Yavuz; Toker, M. Berk; TOKER, MEHMED BERK; Bursa Uludağ Üniversitesi/Veteriner Fakültesi.; 0000-0003-4033-9749; A-2794-2014
The complexity of the sheep cervix limits non-surgical artificial insemination and embryo production technologies. For this reason, assisted reproduction techniques are generally performed with surgical methods in sheep. But it is said that surgical methods can hurt the health and welfare of animals and cause them to feel stressed in different ways. Because of these problems with surgical methods and some difficulties in the application phase, researchers are trying to come up with ways to help with reproduction that don't involve surgery. For the application of non-surgical assisted reproductive techniques in sheep, there is a need for successful relaxation of the cervix. Because of this, different tocolytic agents have been used before non-surgical methods of assisted reproduction. Isoxsuprine HCl is used to relax the uterus during procedures like simple dystocia, to prevent premature birth, embryotomies, and caesarean deliveries. It is also used to treat horse navicular disease and laminitis in modern veterinary medicine. Tocolysis usually sets in about 10-15 minutes after an intramuscular isoxsuprine HCl administration. The aim of the present study was to evaluate the effects of isoxsuprine HCl on cervical dilatation in ewes. In our study, it has been thought that isoxsuprine HCl, which is also a tocolytic agent, might be an alternative for non-surgical reproductive uses. The study was carried out on a total of 20 animals: Ten sheep were given cervical relaxation with isoxsuprine HCl and ten animals were given no tocolytic agent. The mean cervical transition time was 83.60 +/- 13.63 seconds in animals treated with isoxsuprine HCl and 168.22 +/- 20.83 seconds in the control group. A significant difference was found between the groups (P<0.05). The minimum transition time was found to be 19 seconds in the isoxsuprine HCl group and 30 seconds in the control group. Maximum transition times were found at 140 and 238 seconds, respectively. As a result, it was seen that isoxsuprine HCl can offer a good alternative in transcervical applications in sheep.
Effect of rainbow trout (Oncorhynchus mykiss) seminal plasma on the post-thaw quality of ram semen cryopreserved in a soybean lecithin-based or egg yolk-based extender
(Elsevier, 2015-11-15) Üstüner, Burcu; Alcay, Selim; Toker, M. Berk; Nur, Zekariya; Gökce, Elif; Sonat, Füsun Ak; Gül, Zülfiye; Duman, Muhammed; Cenize, Cafer; Uslu, Aydın; Saığrkaya, Hakan; Soylu, Mustafa Kemal; Uludağ Üniversitesi/Veteriner Fakültesi/Üreme ve Suni Tohumlama Anabilim Dalı.; Uludağ Üniversitesi/Veteriner Fakültesi/Fizyoloji Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Farmakoloji Anabilim Dalı.; Uludağ Üniversitesi/Veteriner Fakültesi/Su Hayvanları Hastalıkları Anabilim Dalı.; 0000-0001-7707-2705; 0000-0002-1438-221X; 0000-0002-8872-0074; 0000-0002-7678-3289; AAH-2635-2021; T-1697-2019; AAH-8821-2021; AAF-9939-2020; AAG-7238-2021; 18937724600; 56099810300; 56480349200; 6508060684; 56779799700; 26428428000; 56086542900; 55568071100; 57070339500; 57069822700; 6602400461; 7003293300
The aim of the current study was to evaluate the effects of different concentrations of rainbow trout seminal plasma (RTSP) (0.1%, 1% and 10%) in extenders containing either egg yolk or lecithin for use in Awassi ram semen cryopreservation. Pooled sperm were diluted in a two-step dilution method to a final concentration of 1/5 (semen/extender) in egg yolk or lecithin extender containing no RTSP, 0.1%, 1% or 10% RTSP (v/v). Semen samples were assessed for sperm motility, plasma membrane integrity [hypoosmotic swelling test (HOST) and Hoechst 33258] and defective acrosomes [FITC-conjugated Pisum sativum agglutinin (PSA-FITC)] at the following five time points: after dilution with extender A; after equilibration; and post-thaw at 0 h, 3 h and 5 h. Malondialdehyde (MDA) was examined only after thawing. Freezing and thawing procedures (dilution, equilibration and post-thaw incubation at Oh, 3 h and 5 h) negatively affected the motility (P<0.001) and acrosome integrity (P<0.001). Additionally, freezing and thawing negatively affected the plasma membrane integrity, as determined by the HOST and Hoechst 33258 (P<0.001). The extender group affected the motility (P<0.001) and the HOST results (P<0.001). Levels of MDA in the egg yolk extender with 1% RTSP group were significantly lower than in the lecithin control group (P<0.05). In conclusion, the egg yolk extender groups that were supplemented with 10% and 1% RTSP provided greater cryoprotective effects for semen survivability during 5 h incubation than the other extender groups.
Effect of sperm pooling with seminal plasma collected in breeding or nonbreeding season on Saanen goat sperm cryosurvival
(Wiley, 2018-05) Üstüner, Burcu; Gökçe, Elif; Toker, M. Berk; Önder, Nail Tekin; Saǧırkaya, Hakan; Nur, Zekariya; Uludağ Üniversitesi/Veteriner Fakültesi/Üreme ve Suni Tohumlama Anabilim Dalı.; 0000-0003-4033-9749; 0000-0002-1438-221X; 0000-0001-5141-0008; AAG-7238-2021; A-2794-2014; AAH-8821-2021; AAH-2635-2021; JAC-3152-2023; 18937724600; 56779799700; 56480349200; 55670728900; 6602400461; 6508060684
The aim of this study was to determine the effects of both the removal of seminal plasma (SP) and the pre-freezing addition of seminal plasma collected during the breeding or nonbreeding season on goat sperm survival after thawing. Semen samples were pooled. One aliquot of pooled semen was used as a control group. Four aliquots were then centrifuged, and the SP was removed in Group I, pipetted but not removed in Group II, removed and then pooled for animals collected in the breeding season in Group III and removed and pooled for animals collected in the nonbreeding season in Group IV. Group samples were frozen and then were assessed for rates of sperm motility, plasma membrane functional integrity hypo-osmotic swelling test (HOST), defective acrosomes (FITC-PSA), DNA fragmentation (TUNEL) and mitochondrial membrane damage (Rhodamine 123). The results showed that pre-freezing addition of SP collected in breeding season maintained post-thaw sperm characteristics at 0hr better than SP removal group, but removing seminal plasma showed positive effects on spermatozoa, as incubation time increased to 5hr. In conclusion, the pre-freezing addition of seminal plasma did not maintain post-thaw goat sperm characteristics as successfully as in the groups with seminal plasma removed after an incubation period.
Freeze-dried egg yolk based extenders containing various antioxidants improve post-thawing quality and incubation resilience of goat spermatozoa
(Elsevier, 2016-03-21) Alçay, Selim; Gökçe, Elif; Toker, M. Berk; Önder, N. Tekin; Üstüner, Burcu; Uzabacı, Ender; Gül, Zülfiye; Çavuş, Seda; Uludağ Üniversitesi/Veteriner Fakültesi/Üreme ve Suni Tohumlama Anabilim Dalı.; Uludağ Üniversitesi/Veteriner Fakültesi/Biyoistatistik Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Farmakoloji Anabilim Dalı.; 0000-0001-5141-0008; 0000-0002-7678-3289; 0000-0002-9634-0055; 0000-0002-8872-0074; AAF-9939-2020; AAG-7238-2021; 56099810300; 56779799700; 56480349200; 55670728900; 18937724600; 55347697800; 56086542900; 57188685710
The aim of this study was to evaluate different antioxidants-supplemented freeze-dried egg yolk based extenders for the post-thawing quality and incubation resilience of goat spermatozoa. Pooled semen were diluted in a two-step dilution method to a final concentration of 1/5 (semen/extender) in control and antoxidant supplemented freeze-dried extenders (methionine, cysteamine and butylated hydroxytoluene). Semen samples were assessed for sperm motility, plasma membrane functional integrity using hypoosmotic swelling test (HOST), damaged acrosome using FITC-Pisum sativum agglutinin (PSA-FITC) and DNA integrity using terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL). Membrane lipid peroxidation status was also analyzed using the malondialdehyde (MDA) concentration. In the study, antioxidant supplemented freeze-dried egg yolk based extenders have beneficial effect on goat sperm parameters. In addition, we achieved a higher quality in post thawed goat semen even after 6 h incubation when the extender was supplemented by 5 mM BHT or cysteamine.
Royal jelly supplemented soybean lecithin-based extenders improve post-thaw quality and incubation resilience of goat spermatozoa
(Elsevier, 2016-11-25) Alçay, Selim; Toker, M. Berk; Önder, N. Tekin; Gökçe, Elif; Uludağ Üniversitesi/Veteriner Fakültesi/Üreme ve Suni Tohumlama Anabilim Dalı.; 0000-0002-7678-3289; 0000-0001-5141-0008; 0000-0003-4033-9749; ABA-6294-2020; 56099810300; 56480349200; 56779799700; 55670728900
The aim of the present study was to evaluate different concentrations of royal jelly (RJ) supplemented extenders for post-thaw quality and incubation resilience of goat spermatozoa. Semen samples were collected from five goats. Pooled semen were diluted with soybean lecithin-based extender without RJ (control) or supplemented with different concentrations (0.25, 0.5 and 0.75%) of RJ (RJ0.25, RJ0.5, RJ0.75 respectively), at a final concentration of 150 x 106 spermatozoon/mL. Semen samples were assessed for sperm motility, plasma membrane integrity using hypoosmotic swelling test (HOST) damaged acrosome using FITC-Pisum sativum agglutinin (PSA-FITC) and DNA integrity using terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL). The addition of RJ (0.5%, 0.75%) led to higher percentages of subjective motilities (55.33 +/- 2.29%, 57.67 +/- 2.58%) compared to control and RJ0.25 groups (49.00 +/- 2,80%, 51.67 +/- 3.09%) (P < 0.05) following the freezethawing process. RJ0.5 and RJ0.75 groups had higher plasma membrane functional integrities (66.40 +/- 1.34%, 68.20 +/- 2.05%) and lower defected acrosome rates (24.60 +/- 3.36%, 23.80 +/- 2.27%) compared to the other groups (P < 0.05). DNA damaged spermatozoa in all groups were not significant (P > 0.05). In the end of incubation, motility and HOST rates of RJ0.5 (14.00 +/- 3.87%, 31.20 +/- 3.70%) and RJ0.75 (15.00 +/- 3.27%, 29.20 +/- 2.59%) groups were higher than control (8.00 +/- 2.54%, 18.20 +/- 3.11%) and RJ0.25 (9.00 +/- 2.07%, 20.60 +/- 2.88%) groups (P < 0.05). Also defected acrosome and DNA fragmation rates of RJ0.5 (32.20 +/- 1.30%, 5.4 +/- 0.55%) and RJ0.75 (29.20 +/- 1.30%, 5.80 +/- 0.45%) groups were significantly lower than control (38.80 +/- 0.84%, 7.40 +/- 1.34%) and RJ0.25 (39.80 +/- 2.05%, 7.00 +/- 1.58) groups. This study shows that RJ supplemented extenders have beneficial effect on goat sperm parameters at 0 h and 6 h of incubation.
Successful ram semen cryopreservation with lyophilized egg yolk-based extender
(Academic Press Inc Elsevier Science, 2015-10) Alçay, Selim; Toker, M. Berk; Gökçe, Elif; Üstüner, Burcu; Önder, Nail Tekin; Sağırkaya, Hakan; Nur, Zekariya; Soylu, M. Kemal; Uludağ Üniversitesi/Veterinerlik Fakültesi/Dölerme ve Suni Tohumlama Anabilim Dalı.; 0000-0002-4690-1029; 0000-0001-6619-3229; 0000-0002-1438-221X; 0000-0002-2472-8157; 0000-0001-5999-4685; AAG-7238-2021; AAH-8821-2021; AAH-2635-2021; 56099810300; 56780223500; 56779799700; 18937724600; 56780268300; 6602400461; 6508060684; 8237440900
The aim of this study was to evaluate the effects of lyophilized egg yolk extender on ram semen cryopreservation. Ejaculates with a thick consistency, rapid wave motion (3-5 on a 0-5 scale) and >75% initial motility were pooled. Sperm were diluted to final concentration of 1/5 (semen/extender) in lyophilized egg yolk or fresh egg yolk extenders using two-step dilution method. The equilibrated semen was frozen in 0.25 mL straws. Semen samples were assessed for sperm motility, plasma membrane functional integrity using hypoosmotic swelling test (HOST), damaged acrosome using FITC-Pisum sativum agglutinin (PSA-FITC) and DNA integrity using terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) at three time points: after dilution with extender A, equilibration and post-thaw. The results showed that freezing and thawing procedures (dilution, equilibration and thawing) had negative effects on motility (P < 0.001), plasma membrane integrity (P < 0.001), acrosome integrity (P < 0.001) and DNA integrity (P < 0.001). In the study, there were no significant differences between lyophilized and fresh egg yolk extenders when comparing motility, plasma membrane integrity, acrosome integrity and DNA integrity between groups. In conclusion, lyophilized egg yolk extender provided similar cryoprotective effects with fresh egg yolk extender to cryopreserve ram semen.