Browsing by Author "Temizel, Ethem Mutlu"
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Item Atopik dermatitisli köpeklerin tanısında intradermal deri testleri ile allerjen spesifik Ig E düzeylerinin karşılaştırılması(Uludağ Üniversitesi, 2005) Temizel, Ethem Mutlu; Aytuğ, Nilüfer; Uludağ Üniversitesi/Sağlık Bilimleri Enstitüsü/İç Hastalıkları Anabilim Dalı.Bu çalışma, kaşıntı şikayeti ile getirilen, klinik olarak atopi şüpheli olan köpeklerde klinik tablonun, ntradermal deri testi ( DDT) ve Enzyme Linked Immunosorbent Assay(ELISA) testi uygulanarak Bursa bölgesinde atopiye neden olan allerjenlerin belirlenmesi,ELISA testi ile elde edilen sonuçların intradermal deri testi sonuçları ile karşılaştırılarak sensitivite-spesifitesi değerlendirilerek, bu testlerin tanıda kullanılabilirliklerinin belirlenmesi amacı ile yapıldı.Çalışmanın materyalini, değişik ırk ve yaş, 26 erkek, 24 dişi olmak üzere toplam 50 köpek oluşturmuştur. Atopi şüpheli köpeklerin klinik muayenelerinin yapılması ve spesifiktanısal testler temelinde olası hastalıklar (piyoderma, uyuz, dermatomikoz ve gıda alerjisi v.b)elimine edildi. Allerjenler, Uludağ Üniversitesi Tıp Fakültesi Allerji Bilim Dalının önerileri doğrultusunda ve daha önce Bursa bölgesinde köpekler üzerinde yapılan bir çalışma temelinde belirlendi. Bu çalışmada kullanılan allerjen ekstraktları toplam 18 allerjen (Çayırotu, söğüt, ısırgan otu, kayın, meşe, çınar, ot miks, pire, köpek epiteli, kedi epiteli, insanepiteli, kümes hayvanları, Aspergillus fumigatus, Penicillum notatum, ev tozu akarları, küfmiks, koyun yünü, pamuk) ile bir pozitif kontrol ve bir negatif kontrol solusyonunu içeriyordu.Serum örneklerinden, ELISA testi ile allerjen spesifik Ig E tayinleri yapıldı.Çalışmada kullanılan tüm köpeklerin intradermal deri testinde kullanılan allerjenler denen az birine veya daha fazlasına pozitif reaksiyon verdikleri saptandı. Çalışmada koyun epiteline % 70 (n:35), ev tozu akarlarına % 66 (n: 33), pireye % 48 (n:24), pamuğa % 46 (n:23), insan epiteline % 32 (n: 16), küf mikse % 24 (n: 12), Penicillum notatum'a % 24 (n: 12),köpek epiteline % 18 (n: 9), meşeye % 14 (n: 7), söğüt ağacına % 12 (n: 6), kedi epiteline %10 (n:5), Aspergillus fumigatus'a % 8 (n: 4), çınar ağacına % 6 (n: 3), ısırgan otuna % 6 (n:3), kanatlı tüy mikse % 6 (n: 3), çayır otuna % 6 (n: 3) ve ot miske karşı % 2 (n: 1) oranındapozitif reaksiyon tespit edildi. DDT sonuçlarına göre kayın ağacına karşı hiç pozitifreaksiyon elde edilmedi.Bu çalışmada, in vitro test sonuçları sırası ile ev tozu akarına % 72 (n: 36), koyun yününe % 32 (n: 16), pamuğa % 16 (n: 8), küf mikse % 16 (n: 8), meşe ağacına % 8 (n: 4), otmikse % 6 (n: 3), söğüt ağacına % 6 (n: 3), , kayın ağacına % 6 (n: 3), pireye % 6 (n: 3), insanepiteline karşı % 4 (n: 2), çınar ağacına % 2 (n: 1), ısırgan otuna % 2 (n: 1), çayır otuna % 2(n: 1), köpek epiteline % 2 (n: 1) ve oranlarında pozitif yanıt alınmıştır. Aspergillusfumigatus, Penicillum notatum, kanatlı tüy miks ve kedi epiteline karşı ELISA test sonuçlarına göre negatif sonuç alınmıştır. ntradermal deri testi sonuçları ile karşılaştırılarak, serolojik monoklonal Ig E testininsensitivite ve spesifitesinin sırası ile % 0-82 ve % 29-100 olduğu belirlendi.Sonuç olarak atopi şüpheli olguların tanısında anamnez ve klinik bulgularla birleştirildiğinde, Willemse'nin kriterlerinin klinik açıdan yadsınamayacak bir öneme sahip olduğu, intradermal deri testinin hala altın standart olarak değerlendirilmesi gerektiği,ELISA testi sensitivite ve spesifitesinin ise ev tozu akarları açısından yüksek olduğu ancak genel olarak irdelendiğinde bu iki test arasındaki korelasyonun oldukça düşük olduğu belirlenmiştir.Publication Butaphosphan and cyanocobalamin treatment of pregnant ewes: Metabolic effects and potential prophylactic effect for pregnancy toxaemia(Elsevier, 2015-04-01) Temizel, Ethem Mutlu; Batmaz, Hasan; Keskin, Abdülkadir; Orman, Abdülkadir; Gençoğlu, Hıdır; Çatık, Serkan; Topal, Onur; TEMİZEL, ETHEM MUTLU; BATMAZ, HASAN; KESKİN, ABDULKADİR; ORMAN, ABDÜLKADİR; GENÇOĞLU, HIDIR; Çatık, Serkan; TOPAL, ONUR; Uludağ Üniversitesi/Veteriner Fakültesi/İç Hastalıkları Anabilim Dalı; 0000-0001-9138-4422; 0000-0002-1933-7354; 0000-0003-1991-8957; HJZ-4775-2023; AAH-1712-2021; AAH-3831-2021; AAG-9134-2021; S-8278-2017; ECV-4083-2022; CHM-0716-2022The effect of administration of butaphosphan and cyanocobalamin on the prophylaxis of pregnancy toxaemia in ewe was investigated. Moreover, the effects of these compounds on body weight gain and biochemical metabolism of pregnant ewe was assessed. A total of 59 pregnant Kivircik crossbred ewes were used in this study. Group I (n: 15) was administered butaphosphan and cyanocobalamin three times before delivery at 1-week intervals. Group II (n: 15) was administered butaphosphan and cyanocobalamin three times before delivery at 3-day intervals. Group III (n: 15) was administered 0.9% NaCl three times before delivery at 1-week intervals. Group IV (n: 14) was administered 0.9%NaCI three times before delivery at 3-day intervals. Six blood samples Were taken from each ewe four times before delivery and two times after delivery. Haematological and biochemical analyses were performed.The levels of BHB and NEFA in groups administered butaphosphan and cyanocobalamin were noticeably lower but there were no statistically significance. Elevated BHB (>0.8 mmol/L), subclinical pregnancies toxaemia were identified in 56.66% in test groups and 72.41% in control groups in all ewes and this was higher in the ewes bearing multiple pregnancies 71.42 in test groups and 82.35% in control groups. Subclinical pregnancy toxaemia in pregnant ewes with twins or triplets is lower than the levels for the control groups, despite the greater lamb counts and weights of the ewes in test groups.Based on our results, it was concluded that the butaphosphan and cyanocobalamin combination could be used as an alternative treatment for the prevention of pregnancy toxaemia. (C) 2015 Elsevier B.V. All rights reserved.Item Clinical, haematological and biochemical findings in Saanen goat kids with naturally occurring heat stroke(George Thieme, 2009) Temizel, Ethem Mutlu; Şentürk, Sezgin; Kasap, Sevim; Uludağ Üniversitesi/Veteriner Fakültesi/Uluslararası Tıp Anabilim Dalı.; Uludağ Üniversitesi/Hayvan Hastanesi/Dahiliye Bölümü.; 0000-0002-2465-9913; AAP-7998-2020; AAH-5069-2021; 26430270100; 56257771200; 36514844800Objective: Description of clinical, biochemical and haematological changes in Saanen goat kids post-exposure to a naturally occurring heatstroke. Material and methods:The experimental group consisted of goats of different age (1-4 months) and sex (8 males, 12 females) with an average weight of 7.2 +/- 3.1 kg. Twenty clinically healthy, three to four months old, Saanen goat kids (sex-ratio 1:1) were used as a control. The average body weight in this group was 9.4 +/- 2.6 kg. Case history, clinical signs, and results of haematology and blood biochemistry were documented in all goat kids. Results: Most common findings were hyperthermia, ataxia, muscle tremor and depression. Increased serum urea, creatinine, potassium and plasma lactate concentrations as well as an increase in aspartate aminotransferase and lactate dehydrogenase activity were observed in goats post-exposure to heat stroke when compared to the control group. Two goat kids died despite supportive treatment. Physical and biochemical blood parameters improved following treatment. Conclusion: This is the first study on heat stroke in Saanen goat kids. Heatstroke may be fatal in Saanen goat kids, despite appropriate treatment, and may lead to secondary complications. Plasma lactate concentration seems to be a reliable indicator for the prognosis of heat stroke in goat kids.Item Clinical, ultrasonography and haematology of aglepristone-induced mid-gestation pregnancy terminations in rabbits(South African Veterinary Association, 2013) Özalp, Gözde Rabia; Temizel, Ethem Mutlu; Batmaz, Elçin Özocak; Uludağ Üniversitesi/Tıp Fakültesi/Cerrahi Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Kadın Hastalıkları ve Doğum Bölümü.; Uludağ Üniversitesi/Tıp Fakültesi/Dahiliye Bölümü.; 0000-0003-4694-6937; AAE-3607-2019; ECV-4083-2022; FOM-0178-2022; 23985710500; 26430270100; 55387315900Aglepristone is a safe abortifacient in cats, dogs and rabbits. Although no serious side effects have been reported, there is no information available about the effects of the medicine on haematological parameters. For the first time clinical and ultrasonographic features and haematological profiles were evaluated in rabbits treated with aglepristone 15 and 16 days after mating. Ten healthy 10-14 month-old New Zealand White female rabbits were mated with fertile bucks and pregnancies were confirmed by ultrasound 15 days later. Of these, 5 does were treated with aglepristone (test group, n = 5) whilst the remaining five (control group, n = 5) were treated with a saline solution (0.9% NaCl). The treatment dose was 10 mg/kg body weight, administered subcutaneously once daily on two consecutive days (day 15 and 16 post mating). Ultrasonographic, clinical and haematological assessments were performed daily. Aglepristone treatment induced embryonic fluid resorptions without foetal death in mid-gestation terminations. Following ultrasonographic and haematological examinations, it was established that aglepristone is a safe abortifacient in rabbits.Item Comparison of effectiveness of parenteral lincomycin/spectinomycin combination and dexpanthenol application in goat kids with contagious ecthyma(Polish Soc Veterinary Sciences Editorial Office, 2018-04-16) Avcı, Oǧuzhan; Yapıcı, Orhan; Kasap, Sevim; Temizel, Ethem Mutlu; Karakuş, Adil Ömer; Büyükcangaz, Esra; Kavukçu, Fatih; Uludağ Üniversitesi/Veteriner Fakültesi/İç Hastalıkları Anabilim Dalı.; Uludağ Üniversitesi/Veteriner Fakültesi/Mikrobiyoloji Anabilim Dalı.; 0000-0002-6834-0808; 0000-0003-4337-577X; AAP-7998-2020; AAL-2323-2020; 26430270100; 57212942021; 36514844800; 25649139400; 57204181576Contagious ecthyma (CE) is a highly contagious viral skin disease that is typically self-limited. Treatment options include topical antiseptics, such as KMNO4, local antibiotics and systemic antibiotics to prevent secondary skin infections. The aim of this study was to compare the effectiveness of the lincomycin/spectinomycin combination and dexpanthenol (Dxp) in goat kids with CE. The study was conducted at a Saanen dairy goat farm in Bursa, Turkey. The owner of the goat herd inquired at the veterinary hospital about the appearance of granulomatous lesions on the muzzles of goat kids. In this study, 24 goat kids (1-month-olds) were used. All animals were subjected to the same conditions. Blood and papule samples were taken from the animals' lips, muzzle and buccal mucosa for virological analysis. Swab samples were taken from the lesions for culture and antibiogram. The animals were divided into three groups. Lesions were clinically scored at days 1, 7 and 15 according to a modified previously used scoring system. Goat kids were equally grouped on the basis of lesions on the buccal mucosa, lips and muzzle. The animals in group A received 15 mg/kg lincomycin/spectinomycin combination (Lypectin*, Vilsan) intramuscularly for 3 consecutive days, group B received 20 mg/kg Dxp (Bepanthen* amp, Bayer, Germany) intramuscularly for 3 consecutive days, and group C received 0.9% NaCl (2 ml), the control treatment. Clinical recoveries were almost equal in all groups, but by day 14, group A showed better recovery than group B and group C. Both study groups also showed better results than the control group for all days. In conclusion, we believe that the lincomycin/spectinomycin combination or dexpanthenol can be useful in the supplementary treatment of CE in goat kids.Item Comparison of pre-and postpartum serum leptin, ghrelin, and lipid levels in sheep(Bilimsel ve Teknolojik Araştırma Kurumu, 2018-03-29) Temizel, Ethem Mutlu; Cihan, Hüseyin; Levent, Pınar; Sarıl, Ahmet; Özarda, Yeşim; Yılmaz, Zeki; Uludağ Üniversitesi/Veteriner Fakültesi/İç Hastalıkları Anabilim Dalı.; Uludağ Üniversitesi/Veteriner Fakültesi/Biyokimya Anabilim Dalı.; 0000-0001-9836-0749; 0000-0003-1544-6438; A-9637-2008; AAL-8873-2021; T-4585-2019; ABH-3742-2020; 26430270100; 6602381681; 56690616700; 57202390224; 35741320500; 35944810500Serum leptin and ghrelin play important roles in energy metabolism and inducing appetite during pregnancy and lactation. We investigated the serum levels of leptin and ghrelin and their relationship with the duration of lactation and serum biochemical responses during the prepartum (last week before lambing) and postpartum periods in sheep. They were also evaluated between pregnant and nonpregnant (control ewes) ewes. For these purposes, pregnant (n = 15) and control Awassi ewes (n = 15) that were 2 years of age were used in this study. Blood samples were collected 1 week before the expected date of lambing (baseline) and for 12 weeks after parturitions. Serum leptin, ghrelin, cholesterol, triglyceride, VLDL, LDL, HDL, NEFA, and phospholipid levels were assayed. Leptin and ghrelin levels of the prepartum period in pregnant and control ewes were 4.5 ng/mL and 3.4 ng/mL (P < 0.05) and 75 pg/mL and 166 pg/ mL (P < 0.01), respectively. The baseline value of ghrelin (81 pg/mL) decreased to 43 pg/mL within 1 week after parturition (P < 0.01). Serum ghrelin levels increased after 1 week. There was also a significant difference in serum ghrelin levels between control ewes and those a week before parturition in pregnant ewes (P < 0.001). The NEFA level was higher in pregnant than in control sheep (P < 0.001). In conclusion, leptin levels tended to decrease during the lactation period, which could be associated with a negative energy balance. Additionally, ghrelin levels tended to gradually increase during lactation to adapt to a negative energy balance. We think that variations in serum leptin and ghrelin might be used to monitor metabolic adaptation during lactation.Item Effect of prednisolone and cetirizine on D. farinae and histamine-induced wheal and flare response in healthy dogs(George Thieme Verlag, 2011) Akhtardanesh, Baharak; Temizel, Ethem Mutlu; Ci̇han, Hüseyin; Aytuǧ, Nilüfer; Uludağ Üniversitesi/Veterinerlik Fakültesi/İç Hastalıkları Anabilim Dalı.; ABH-3742-2020; 26430270100; 6602381681; 6505835923Objective: Prednisolone and antihistamines are highly potent drugs in the treatment of atopic dermatitis and widely used in humans and dogs. In some atopic patients in which antihistamines, corticosteroids or other drugs have already been administered intradermal testing (IDT) may be necessary. The aim of the present study was to compare the effects of cetirizine and prednisolone on IDT results. Material and methods: Thirty healthy dogs (average age 5.9 +/- 0.6 years) were randomly assigned to three groups. Treatment groups were administered prednisolone (1 mg/kg BW daily, tapering dosage; group I), cetirizine (1 mg/kg BW daily; group II) and placebo (group III) respectively for one week. In the second week, none of the dogs received any medications. IDT was performed prior to drug administration and results obtained were considered as the baseline response. Second and third IDTs were performed at the end of the first and second week, respectively. Results: In groups I and II IDT reactivity was reduced at the end of first week (p < 0.05). After drug discontinuation the reactivity almost returned to baseline at the end of the 2-week period, with the exception of the prednisolone group for D. farinae. Conclusion: Prednisolone and cetirizine have significant effects on IDI reactions and must be withdrawn by veterinary practitioners up to 2 weeks prior to IDT.Item Effect of treatment with cylindamycine in an outbreak of coccidiosis in goat kids in Turkey(Uludağ Üniversitesi, 2011) Temizel, Ethem Mutlu; Demir, Gülşah; Selçuk, Özgür; Çatık, Serkan; Şenlik, Bayram; Şentürk, Sezgin; Uludağ Üniversitesi/Veteriner Fakültesi/İç Hastalıkları Anabilim Dalı.; Uludağ Üniversitesi/Veteriner Fakültesi/Parazitoloji Anabilim Dalı.The purpose of the present study was to investigate the effect of Cylindamycine in saanen goats kids suffering from naturally occuring coccidiosis. 13 kids were found to be suffering from different degrees of coccidiosis. Fecal samples were submitted from all of the diarrheic kids in the goat flock for virological, bacteriological and coccidial examinations. Bacteriogical cultures and flotation examinations of faecal samples were evaluated. In clinical examinations, all kids showed dysentery, tenesmus, inappetence, and weakness. While total per oocyste counts were detected as 675500 opg (per gram oocysts) before the treatment, were detected as low as 24020 opg at end of day 14. Clindamycine applications in addition to managemental measurements may be useful to reduce the oocyst counts and to improve of clinical status.Item Effects of levamisole and ranitidine on antibody-forming responses induced by killed Mycoplasma vaccine antigens in Saanen goats(Wiley, 2012-12-08) Temizel, Ethem Mutlu; Önat, Kaan; Meci̇toğlu, Zafer; Kasap, Sevim C.; Göçmen, Hüban; Ülgen, Mihriban; Uludağ Üniversitesi/Veteriner Fakültesi/İç Hastalıkları Anabilim Dalı.; Uludağ Üniversitesi/Veteriner Fakültesi/Mikrobiyoloji Anabilim Dalı.; 0000-0002-6307-5179; 0000-0002-5557-121X; 0000-0002-2245-5781; AAG-8117-2021; AAP-7998-2020; AAH-1677-2021; B-9095-2018; 26430270100; 24069071700; 36457647300; 36514844800; 56414715500; 56259664500Item Efficacy of tylosine against clinical cryptosporidiosis in goat kids(Univ Agriculture, 2011) Temizel, Ethem Mutlu; Şentürk, Sezgin; Girişgin, Onur A.; Şenlik, Bayram; Demir, Gülşah; Uludağ Üniversitesi/Veterinerlik Fakültesi/İç Hastalıkları Anabilim Dalı.; Uludağ Üniversitesi/Veterinerlik Fakültesi/Parazitoloji Anabilim Dalı.; 0000-0002-2465-9913; 0000-0002-0020-2708; 0000-0003-2964-2245; AAH-5069-2021; B-5286-2017; 26430270100; 56257771200; 36011813800; 9332720500; 36542081400The aim of the present study was to evaluate tylosine efficacy administered by intramuscular for treatment of cryptosporidiosis in naturally infected goat kids. These animals were randomly assigned to test group (n=10) and control (n=10) group after routine clinical examination. All kids showed mild mental depression, decrease in suckling reflex and diarrhea in different severity. Fecal samples were analyzed via virological, bacteriological and coccidial examinations. The consistency of feces was assessed as pastose, semiliquid, or liquid. The rates of infection of the samples were evaluated semi-quantitatively. Tylosine was given by intramuscular route to test group - 2 at a dosage of 10 mg/kg of body weight, twice a day for 5 days. However, isotonic saline solution (1.0 ml) was used by intramuscular route to the control group, once a day for 5 days. Hematological results of all the kids were within normal limits except for hematocrit rates which were mild high in 15 kids as a result of dehydration. However, OPG was not detected in both of group after treatment. It was suggests that tylosine applied animals has been shown more rapid recovery than control group. Tylosine may be useful in order to reduce of treatment period in the disease. In future, more detailed studies which evaluate the effects of tylosine in goat kids with cryptosporidiosis are needed.Item Enfektif ishalli ve sağlıklı neonatal buzağılarda serum amyloid A, serum calprotectin ve ve fekal calprotectin arasındaki ilişkilerin ve inflamatuvar marker olarak diagnostik önemlerinin belirlenmesi(Bursa Uludağ Üniversitesi, 2021-07-05) Karakuş, Adil Ömer; Temizel, Ethem Mutlu; Bursa Uludağ Üniversitesi/Sağlık Bilimleri Enstitüsü/Veteriner İç Hastalıkları Anabilim Dalı.; 0000-0002-6834-0808Bu çalışmada neonatal dönemdeki enfektif ishalli buzağılarda serum amyloid A (SAA), serum calprotectin (SCalp) ve fekal calprotectin (FCalp) düzeyleri araştırıldı. Çalışma gruplarını etiyolojik teşhislere göre, E.coli (n=17), C.parvum (n=11), C. Parvum + viral (mix) (n=8), viral (n=19) ve kontrol grubu (n=15) olacak şekilde toplam 70 buzağı oluşturdu. İshalin ilk tespit edildiği an olan 0. günde ve tedavi sonrası 7. günde buzağıların vücut sıcaklığı, respirasyon ve pulzasyon sayıları, dışkı, emme ve mental statü skorları kayıt altına alındı. Bu günlerde ayrıca kan ve dışkı örnekleri alındı. 0. gün kan örneklerinden WBC, serum GGT, SAA ve SCalp; dışkı örneklerinden ise FCalp düzeyleri belirlendi. Bütün klinik parametrelerde 7.günde istatistiksel olarak anlamlı düzelmeler saptandı. 0.günde ölçülen ortalama SAA konsantrasyonları, ishalli ve sağlıklı grup için sırası ile 0,54 (0,16 – 2,18) ng/ml ve 38,40 (8,28 – 83,96) ng/ml olarak ölçüldü ve iki ölçüm günü arasında istatistiksel fark elde edildi (P < 0,001). Sunulan çalışmada 0. gün SCalp düzeyleri açısından ishalli (68,02 ng/ml) ve kontrol (24,05 ng/ml) grubu arasında anlamlı istatistiksel fark elde edildi (P < 0,001). E.coli pozitif buzağıların oluşturduğu gruptaki SCalp ve FCalp değerleri diğer etiyolojik etkenlerdeki düzeylere göre daha yüksek saptandı. Ayrıca bakteriyel ve viral kökenli ishal vakaları ayırt etme gücünün tespiti için yapılan ROC analizinde SCalp’ın cut-off değeri ≤ 70,969 ng/ml (Sens: %94, Spec: 62,5, AUC: 0,842, P=0,002) olarak bulundu. FCalp düzeyleri 0.günde ishalli ve sağlıklı grupta sırasıyla, 98,23 ng/ml ve 87,91 ng/ml olarak bulundu ve iki grup arasında istatistiksel fark elde edildi (P = 0,01). ROC analizlerine göre FCalp’ın cut-off konsantrasyonu > 91,804 ng/ml olarak bulundu (AUC: 0,694, P = 0,0057). Ayrıca yapılan lojistik regresyon analizinde, 0.gün FCalp Odds Ratio değerinin 6,316 (P = 0,009) olduğu belirlendi. Bu çalışmada; veteriner literatüründe buzağı ve sığırlarda ilk kez değerlendirilen FCalp ve SCalp’ın önemleri vurgulanmaktadır. Bu çalışmada, buzağılarda enfektif intestinal hastalıklarda, SCalp ve FCalp ile elde edilen verilerin ileride yapılacak çalışmalara ışık tutacağı, sahada kullanışlı bir parametre olarak yangısal sürecin monitarizasyonunda kullanılabileceği düşünülmektedir.Item Epizootic hemorrhagic disease in cattle, Western Turkey(Centers Disease Control & Prevention, 2009-02) Batten, Carrie; Maan, Narender Singh; Mertens, Peter; Temizel, Ethem Mutlu; Yeşilbağ, Kadir; Şentürk, Sezgin; Batmaz, Hasan; Uludağ Üniversitesi/Veteriner Fakültesi/İç Hastalıkları Anabilim Dalı.; Uludağ Üniversitesi/Veterinerlik Fakültesi.; 0000-0003-1991-8957; 0000-0002-2465-9913; AAH-1712-2021; AAH-5069-2021; ABE-7662-2020; 26430270100; 6602912127; 56257771200; 6602783183In 2007, an outbreak of epizootic hemorrhagic disease (EHD) occurred in Turkey. On the basis of clinical investigation, 41 cattle were suspected to have EHD. Reverse transcription-PCR and sequence analyses indicated that the virus belonged to EHD virus serotype 6, thus confirming EHD virus infection of cattle in Turkey.Item Evaluation of erythrocyte and platelet indices in canine visceral leishmaniasis(Ankara Üniversitesi, 2011) Temizel, Ethem Mutlu; Ci̇han, Hüseyin; Yılmaz, Zeki; Aytuǧ, Nilüfer; Uludağ Üniversitesi/Veterinerlik Fakültesi/İç Hastalıkları Anabilim Dalı.; 26430270100; 6602381681; 35944810500; 6505835923The purpose of the study was to evaluate erythrocyte indices and platelet indices in dogs with leishmaniasis (CL, L. infantum). CL was diagnosed by polymerase chain reaction, and confirmed by western blott in 54 dogs. These dogs were divided into three groups; asymptomatic (n=16), oligosymptomatic (n=16), and symptomatic (n=22). Leishmaniasis free 25 dogs were served as controls. Microcytic or normocytic-hypochromic anemia was observed in dogs with CL. There was not a significant difference on RDW (RBC distribution width) groups. PLT count was slightly lower in dogs with CL in all clinical stages (p<0.05), compared to controls. MPV (mean platelet volume) in dogs with CL was higher (P<0.05) compared with controls, and it was significantly higher in asymptomatic dogs. RBC (red blood cell) and platelet indices may be useful parameters during course of the leismaniasis. These indices may have diagnostic properties but are not enough to differentiate of the clinical stages of CL. Further comprehensive studies are needed to clinical use of RBC and platelets indices in canine practice.Item Evaluation of faecal shedding of acid-fast mycobacterium avium subsp. paratuberculosis (map) in both intradermal johnin test- and serologically (Elisa) mappositive cattle(Uludağ Üniversitesi, 2012-11-22) Cihan, Hüseyin; Mecitoğlu, Zafer; Demir, Gülşah; Temizel, Ethem Mutlu; Şentürk, Sezgin; Uludağ Üniversitesi/Veteriner Fakültesi.Paratuberculosis is a debilitating disease of livestock caused by Mycobacterium avium (MAP). As a result of economic impact caused, disease is now one of the most important problems of dairy industry. Detection of animals shedding MAP organism plays a crucial role in herd eradication of the disease. Aim of the presented study was to evaluate relationship between MAP ELISA results, Intradermal Johnin test and fecal MAP shedding ratio. 40 ELISA and Johnin test positive animals were selected for the study. 25 of 40 animals (62.5%) were shedding MAP organisms with their feces. Results of the present study indicate that combination ELISA and Johnin test results is a useful tool for prediction of animals shedding MAP.Item Evaluation of serum iron and iron binding capacity in cows with paratuberculosis(George Thieme, 2009) Şentürk, Sezgin; Mecitoğlu, Zafer; Ülgen, Mihriban; Borum, Ebru; Temizel, Ethem Mutlu; Kasap, Sevim; Uludağ Üniversitesi/Veterinerlik Fakültesi/İç Hastalıkları Anabilim Dalı.; Uludağ Üniversitesi/Veterinerlik Fakültesi/Mikrobiyoloji Anabilim Dalı.; 0000-0002-2465-9913; 0000-0002-6307-5179; AAH-5069-2021; AAP-7998-2020; AAG-8117-2021; 56257771200; 36055033100; 56259664500; 36738850800; 26430270100; 36514844800Objective: The purpose of the study was to evaluate the results of routine haematological, serum iron and iron binding capacity analyses in cattle with Mycobacterium avium subsp. paratuberculosis (MAP) infection. Material and methods: Thirty-eight cows were included in the study. An ELISA for MAP, intradermal Johnin test and microscopic examination of the animals faecal smears for acid fast organisms were performed. According to the results 19 cows (4-8 years old) were found positive and 19 cows (4-7 years old) from the same herd were negative and served as control group. In all cows routine haematological values as well as serum iron level and iron binding capacity were evaluated. Results: When compared with control animals, routine haematological values including haematocrit, haemoglobin and erythrocyte counts were significantly (p < 0.05) lower in positive cows. Also serum iron level (p < 0,01) and iron binding capacity (p < 0.01) were significantly lower in positive cows than in the control group. Conclusion: As a result, these parameters can be important for the mechanism of the pathogenesis of paratuberculosis.Item Floppy kid disease: Diagnostic and therapeutic approach in kids suffering from FKD in Northern Cyprus(Uludağ Üniversitesi, 2017-12-08) Sayıner, Serkan; Temizel, Ethem Mutlu; Kavukçu, Fatih; Karakuş, Adil Ömer; Uludağ Üniversitesi/Veteriner Fakültesi/İç Hastalıkları Anabilim Dalı.Floppy Kid Disease (FKD) is a condition which is characterized with metabolic acidosis, generalized anorexia and muscle weakness that can be seen in young goat kids. During the course of an investigation on goat kids suffering from muscle weakness and increased deaths in a goat farm in Cyprus, 10 kids were evaluated. Hematological and biochemical examinations were performed for clinically depressive 5 kids. Also, blood gas analysis were performed for clinically depressive other 5 kids on the next day. As a result of the evaluation, blood pH values of 3 kids were 7.35, 7.33 and 7.26 in blood gas analysis and in addition to that base excess values of these kids were -3.90, -4.0 and -6.8, respectively. Furthermore, BHB values of these kids, which can be generated as a mid-product of D Lactate, were high and especially the kid with a pH value of 7.26 had BHB level of 160 μmol/L. Following the treatment, only 2 kids died during 1 month of follow-up treatment. This study emphasizes the importance of metabolic acidosis and related parameters in diagnosis of FKD. In general, separation of kids from nannies and giving them oral bicarbonate were thought to be effective for Floppy Kid Disease.Publication IGF-1 and GH alterations in lambs with intestinal inflammation(TÜBİTAK, 2021-01-01) Temizel, Ethem Mutlu; Karakuş, Adil Ömer; Kavukcu, Fatih; Küçükşen, Duygu Udum; Ercan, İlker; TEMİZEL, ETHEM MUTLU; Karakuş, Adil Ömer; Kavukcu, Fatih; Küçükşen, Duygu Udum; ERCAN, İLKER; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/İç Hastalıkları Anabilim Dalı.; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Biyokimya Anabilim Dalı.; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Biyoistatistik Anabilim Dalı.; 0000-0002-6834-0808; 0000-0002-2382-290X; ECV-4083-2022; FBQ-7974-2022; JJZ-4815-2023; JJM-7084-2023; ABF-2367-2020Insulin-like growth factor-1 (IGF-1) acts on the development of internal organs such as the small intestine and muscle in all animal species. Similar to IGF-1, GH is also essential for growth and is an effective hormone on intestinal development during neonatal period. The aim of this study was to investigate the effects of diarrhea on IGF-1 and GH hormones in lambs with intestinal inflammation up to 63 days of age. The study material consisted of 15 healthy and 15 diarrheic lambs. Blood and fecal samples were collected on the first day of life and on the 7th, 14th, 21st, 35th, 49th and 63rd postnatal days consecutively. Diarrhea was observed on 6 animals on the 7th day and 9 animals on 14th day visitation. IGF-1 showed statistically significant differences (P < 0.01) between diarrheic and healthy animals on all measurement days. A significant association was found between the cut-off values determined after ROC analysis of the 7th day (Sens: 93.33%, Spec: 66.67%, P = 0.004) and 14th day (Sens: 73.33%, Spec: 80%, P = 0.0002) values of IGF-1 and diarrhea. Considering 14th day of IGF-1, a logistic regression analysis was performed, the risk of diarrhea is OR = 7.00 times higher if the concentration of the parameter is above the cut-off value. ROC analysis also showed significant cut-off values for GH on 14th day. In terms of sensitivity, the highest value was IGF-1's 7th day value, therefore it can be preferred for the determination of intestinal inflammation in cases with diarrhea. As a general evaluation, it is seen that the highest performance was given by the 7th day IGF-1 values according to the Youden's J index. In conclusion, elevations in IGF-1 and GH concentrations may be associated with intestinal inflammation. The inducing effect of the inflammatory response on IGF-I and GH may strengthen the relationship between the two parameters.Publication Immune response of cattle to botulinum type C and D toxoid administered on three occasions(Univ Agriculture, Fac Veterinary Science, 2015-01-01) Mecitoğlu, Zafer; Temizel, Ethem Mutlu; Özyigit, Özgür; Akgül, Gülşah; Kennerman, Engin; MECİTOĞLU, ZAFER; TEMİZEL, ETHEM MUTLU; ÖZYİĞİT, MUSA ÖZGÜR; Akgül, Gülşah; KENNERMAN, ENGİN; Uludağ Üniversitesi/Veteriner Fakültesi/Patoloji Anabilim Dalı; Uludağ Üniversitesi/Veteriner Fakültesi/Dahiliye Anabilim Dalı; AAH-1677-2021; ECV-4083-2022; GLP-3036-2022; CBU-1718-2022; FDU-5862-2022The aims of the present study were to investigate the antibody response of cows from an outbreak region to vaccination with a bivalent botulinum toxoid (Type C and D) on three occasions and to investigate the antibody response to vaccination with the botulinum toxoid in the presence of anti-BoNT antibodies. Twenty-two cows from an outbreak region and 10 cows from a farm without a history of botulism were used in this study. Blood samples were collected before each vaccination and three weeks after the third vaccination (days 0, 21, 42 and 63). The level of anti-BoNT/C antibodies steadily increased after each vaccination (0.471 +/- 0.04, 0.566 +/- 0.03 and 0.663 +/- 0.04, respectively); however, the levels of anti-BoNT/ D antibodies were not significantly different after the second and third vaccinations (0.377 +/- 0.03, 0.493 +/- 0.03 and 0.465 +/- 0.03, respectively). Post vaccination antibody responses of animals found positive and negative for anti-BoNT antibodies at the beginning of the study were similar. The results of the present study indicated that vaccination of cattle with botulinum toxoid on three occasions is recommended, particularly in outbreaks that are suspected to be caused by BoNT/C and that presence of naturally acquired antibodies against BoNT did not interfere with post vaccination immune response.Item Iodine intoxication in beef cattle in Turkey - clinical, hematological and biochemical evaluation(Hellenic Veterinary Medical Soc, 2018) Şentürk, Sezgin; Temizel, Ethem Mutlu; Karakuş, Adil Ömer; Kasap, Sevim; Akkaya, Fatma; Uludağ Üniversitesi/Veteriner Fakültesi/İç Hastalıkları Anabilim Dalı.; 0000-0002-2465-9913; 0000-0001-6536-5594; AAH-5069-2021; F-4850-2017; AAP-7998-2020; 56257771200; 26430270100; 57212942021; 36514844800; 57217582317Iodine is an essential trace element for humans and animals. The incidence of iodine poisoning in cattle is low. In the present study, we evaluated the clinical findings, serum glucose and cholesterol, thyroid hormone and urine iodine levels in cattle exposed to excess iodine. All of the clinical data were determined following the addition of potassium iodide to the drinking water. Inappetence, cough, and hyperthermia were notable clinical findings. We detected a very high iodine level (470 mu g /L) in an analysis of the drinking water samples. A biochemical analysis revealed that the serum cholesterol levels in the affected cattle were significantly lower (p<0.05) than in healthy cattle. However, the serum glucose in the affected cattle was significantly higher (p<0.05) compared to healthy cattle. The iodine concentration in the urine of the affected animals was also significantly higher (p<0.05) than in the healthy animals. Importantly, a hematological analysis indicated leukocytosis with neutrophilia. Several clinical signs, including hyperthermia, tachycardia, alopecia, and a naso-oral discharge, based on suspected history can suggest iodine intoxication. In addition, biochemical parameters, such as urine iodine, serum glucose and cholesterol levels, were observed to be different between healthy and affected cattle. The thyroid function in affected cattle should also be studied.Item İshalli ve sağlıklı kuzularda pasif transfer durumu, serum growth hormon ve insulin like growth faktör -1 düzeyi arasındaki ilişkinin değerlendirilmesi(Uludağ Üniversitesi, 2018-12-26) Kavukcu, Fatih; Temizel, Ethem Mutlu; Bursa Uludağ Üniversitesi/Sağlık Bilimleri Enstitüsü/İç Hastalıkları Anabilim Dalı.Sunulan çalışmada, neonatal periyot ve sonrasındaki erken dönemde (Insulin-like Growth Factor-I) IGF-1 ve (Growth Hormone) GH değişimlerinin irdelenmesi, pasif transfer yetmezliğinin (PTY) tespitinde kullanılacak pratik testlerin IGF-1 ve GH değerleri arasındaki ilişkilerinin belirlenmesi amacıyla yapıldı. Çalışma, Bursa'nın Karacabey ilçesinde özel bir koyun çiftliğinde gerçekleştirildi. Çalışma materyalini, 15 ishalli, 15 sağlıklı olmak üzere toplam 30 adet kıvırcık ırkı kuzu oluşturdu. İshalli ve sağlıklı kuzuların oluşturduğu gruplarda, sağlıklı grupta 8 erkek ve 7 dişi, ishalli grupta 7 erkek ve 8 dişi kuzu, ikizlik-tek oranları her iki grupta eşit olacak şekilde dağıtıldı. Hayvanlar, kolostrum alımını takiben doğum anı, 7., 14., 21., 35., 49. ve 63. günlerde klinik durumları takip edilerek, canlı ağırlık ölçümleri yapıldı, ayrıca serumluk ve EDTA'lık kan örnekleriyle, dışkı örnekleri alındı. Alınan kan örneklerinden IGF-1 ve GH düzeyleri ELISA yöntemiyle, (Gamma Glutamyl Transferase) GGT reflotron cihazıyla kuru sistemde, (Gluteraldehit Koagülasyon Testi) GKT süresi ise taze hazırlanan GKT solüsyonu ile manuel olarak ve Total Protein düzeyleri ise Refraktometre ile manuel olarak ölçüldü. Sunulan çalışmada, özellikle doğum zamanı, 49. ve 63. günlerde canlı ağırlık düzeyleri arasında istatistiki olarak anlamlı farklılık olduğu saptandı (p<0.05). Hayvanların hepsinde Kriptosporidiozis. pozitif olarak tespit edildi. IGF-1 için (p<0,05; 0,01) her kan alma döneminde ve GH için ise 14. ve 63. günlerde ishalli grupta sağlıklı gruba göre daha yüksek düzeyde tespit edilmiştir. Sonuç olarak, çalışmanın verileri doğrultusunda literatürdeki bazı bulguların aksine intestinal hasar meydana getirebilen Kriptosporidiozis ile ilişkili olarak IGF-1 ve GH düzeylerinde artış olabileceği ortaya konmuştur.