Browsing by Author "Oral, Barbaros"
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Item Akut izole sfenoidit(Uludağ Üniversitesi, 2003-02-04) Yılmaz, Emel; Erişen, Levent; Oral, Barbaros; Helvacı, Safiye; Aydın, Selçuk; Aksoy, Kaya; Uludağ Üniversitesi/Tıp Fakültesi/Nöroşirurji Bilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Mikrobiyoloji Anabilim dalı.; Uludağ Üniversitesi/Tıp Fakültesi/KBB Anabilim dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Mikrobiyoloji ve Enfeksiyon Hastalıkları Anabilim dalı.Menenjit ve hipofiz tutulumu ile bulgu veren akut izole sfenoid sinüzit olgusu endoskopik cerrahi girişimin önemi vurgulanarak tartışılmıştır.Item Association of breast cancer and cytokine gene polymorphism in Turkish women(Saudi Medical Journal, 2007-11) Gönüllü, Güzin; Baştürk, Bilkay; Evrensel, Türkkan; Oral, Barbaros; Gözkaman, Ayşe; Manavoğlu, Osman; Uludağ Üniversitesi/Tıp Fakültesi/Onkoloji Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Immünoloji Anabilim Dalı.; 6603942124; 7004498001; 9633205800; 6602587152Objective: To investigate the association of cytokine gene polymorphism with the development of breast cancer. Methods: The study was carried out in Uludag University Medical School, Bursa, Turkey. The study included 38 patients with breast cancer admitted to the Medical Oncology outpatient clinic, and 24 healthy controls, age and sex matched, from the Internal Medicine Department between 2004 and 2005. All genotyping of tumor necrosis factor-cc (TNF-alpha), tumor growth factor-beta 1 (TGF-beta 1), interleukin (IL)-10, IL-6, and interferon-gamma (IFN-gamma) experiments were performed using polymerase chain reaction sequence-specific primers. Results: The frequencies of IL-6-174GC genotype and IL-10 (-1082, -819, -592) GCC/ATA haplotype were significantly higher in the patient group (p=0.0008) when compared with controls (p=0.020). Significantly lower frequencies of IL-10 (-1082,-819,-592) ACC/ATA haplotype were observed in the patient group in comparison to the controls (p=0.026). The distribution of IFN-gamma +874, TNF-a 308, and TGF-beta 1 codon 10-25 genotypes failed to show any statistical significant association with the development of breast cancer. Conclusion: Our data suggest that IL-10 (-1082, -819, -592) GCC/ATA haplotype and IL-6-174 GC genotype seem to be potential risk factors for the development of breast cancer. The presence of IL-10ACC/ATA haplotype may be protective for the oncogenesis of breast cancer.Item Can cytokine gene polymorphisms affect the development of epithelial tumors?(Elsevier, 2005) Gönüllü, Güzin; Baştürk, Bilkay; Yavaşçaoğlu, İsmet; Göral, Güher; Oral, Barbaros; Uludağ Üniversitesi/Tıp Fakültesi/Onkoloji Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Üroloji Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Mikrobiyoloji ve Enfeksiyon Anabilim Dalı/İmmünoloji Bölümü.; 0000-0002-8784-1974; 0000-0003-0463-6818; AAD-6918-2021; K-7285-2012Item Cytokine signal suppressor (SOCS) 1-1478 CA/del gene polymorphism in Turkish patients with polycystic ovary syndrome(Taylor & Francis, 2017-10-03) Gül, Cuma Bülent; Gül, Özen Öz; Cander, Soner; Budak, Ferah; Oral, Barbaros; Ersoy, Canan; Uludağ Üniversitesi/Tıp Fakültesi/Endokrinoloji ve Metabolizma Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/İmmünoloji Anabilim Dalı.; 0000-0001-7625-9148; 0000-0003-0463-6818; AAI-1005-2021; F-4657-2014; K-7285-2012; AAH-8861-2021; 26040787100; 25027068600; 6701913697; 7004498001; 6701485882Eighty-four subjects, premenopausal female patients (n=42, mean (SD) age: 26.4 (4.2) years) diagnosed with polycystic ovary syndrome (PCOS) and age-matched healthy volunteers (n=42, mean (SD) age: 27.6(3.4) years), were included in this study. Data on physical examination, anthropometric measurements and blood biochemistry analysis were recorded for each subject along with analysis for SOCS1-1478 CA/del polymorphism by polymerase chain reaction-restriction fragment length polymorphism. The relation of SOCS1-1478 CA/del polymorphism to PCOS status and insulin resistance was analysed via logistic regression analysis. Mean (SD) levels for BMI (28.5(6.5) vs.22.5 (4.9) kg/m(2), p<.001), HOMA-IR (3.1(1.8) vs.1.5 (1.0), p<.001), LDL-cholesterol (115.9(32.7) vs.100.7 (27.3)mg/dL, p=.03) and triglyceride (113.8(64.9) vs.83.3(36.3)mg/dL, p=.017) were significantly higher in patients. Groups were similar in terms of SOCS1-1478 CA/del polymorphism. No significant relation of this polymorphism was noted to PCOS and HOMA-IR. Our findings revealed no difference between groups in terms of the rate of SOCS1-1478 CA/del polymorphism, and no significant relation of this polymorphism to insulin resistance and PCOS status. IMPACT STATEMENT Polycystic ovary syndrome (PCOS), the most common cause of anovulation and the most commonly encountered form of female endocrine disease. SOCS proteins have been suggested to play a fundamental role in the negative feedback regulation of the JAK-STAT pathway, which is the major signalling pathway involved in a wide range of physiologic and pathologic processes, including inflammatory diseases, malignancies and immune disorders. Pathways involving the induction of suppression of SOCS proteins were also shown likely to be involved in mediating cytokine-induced insulin resistance. The present study was designed to determine the frequency of SOCS1-1478 CA/del gene polymorphism in patients with PCOS in relation to healthy controls and insulin resistance. Our findings revealed significantly higher rates of insulin resistance, obesity and dyslipidaemia in Turkish patients with PCOS compared with age-matched healthy controls, while no difference between study groups in terms of the rate of SOCS1-1478 CA/del polymorphism along with no significant relation of SOCS1-1478 CA/del polymorphism to insulin resistance and PCOS status. Future larger scale studies with the application of standardised diagnostic methods and criteria, and of state-of-the-art modern techniques including genomics, proteomics and pharmacogenetics would provide better understanding of the association between PCOS and genomic variants.Publication Flow cytometric analysis of T, B, and NK cells antigens in patients with mycosis fungoides(Hindawi, 2015-11-24) Yazıcı, Serkan; Başkan, Emel Bülbül; Budak, Ferah; Oral, Barbaros; Adım, Şaduman Balaban; Kalın, Zübeyde Ceylan; Özkaya, Güven; Aydoğan, Kenan; Sarıcaoğlu, Hayriye; Tunalı, Şükran; YAZİCİ, SERKAN; BÜLBÜL BAŞKAN, EMEL; BUDAK, FERAH; ORAL, HALUK BARBAROS; BALABAN ADIM, ŞADUMAN; Kalın, Zübeyde Ceylan; ÖZKAYA, GÜVEN; AYDOĞAN, KENAN; SARICAOĞLU, HAYRİYE; Tunalı, Şükran; Uludağ Üniversitesi/Tıp Fakültesi/Dermatoloji ve Zührevi Hastalıklar Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/İmmünoloji Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Patoloji Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Biyoistatistik Anabilim Dalı.; 0000-0001-6407-0962; 0000-0001-7625-9148; 0000-0003-0463-6818; 0000-0003-0297-846X; 0000-0002-0193-1128; IZP-9398-2023; AAH-2459-2021; AAH-1388-2021; A-4421-2016; F-4657-2014; AAH-6216-2021; K-7285-2012; EMN-0789-2022; CWV-8710-2022; DPU-8534-2022; GGH-7385-2022We retrospectively analyzed the clinicopathological correlation and prognostic value of cell surface antigens expressed by peripheral blood mononuclear cells in patients with mycosis fungoides (MF). 121 consecutive MF patients were included in this study. All patients had peripheral blood flow cytometry as part of their first visit. TNMB and histopathological staging of the cases were retrospectively performed in accordance with International Society forCutaneous Lymphomas/European Organization of Research and Treatment of Cancer (ISCL/EORTC) criteria at the time of flow cytometry sampling. To determine prognostic value of cell surface antigens, cases were divided into two groups as stable and progressive disease. 17 flow cytometric analyses of 17 parapsoriasis (PP) and 11 analyses of 11 benign erythrodermic patients were included as control groups. Fluorescent labeled monoclonal antibodies were used to detect cell surface antigens: T cells (CD3(+), CD4(+), CD8(+), TCR alpha beta(+), TCR gamma delta(+), CD7(+), CD4(+) CD7(+), CD4(+) CD7(-), and CD71(+)), B cells (HLA-DR+, CD19(+), and HLA-DR(+)CD19(+)), NKT cells (CD3(+)CD16(+)CD56(+)), and NK cells (CD3(-)CD16(+)CD56(+)). The mean value of all cell surface antigens was not statistically significant between parapsoriasis and MF groups. Along with an increase in cases of MF stage statistically significant difference was found between the mean values of cell surface antigens. Flow cytometric analysis of peripheral blood cell surface antigens in patients with mycosis fungoides may contribute to predicting disease stage and progression.Publication Il-21: A potential biomarker for diagnosis and predicting prognosis in covid-19 patients(European Respiratory Society Journals, 2021-09-05) Öztürk, Nilüfer Aylin Acet; Ursavaş, Ahmet; Dilektaşlı, Aslı Görek; Demirdöğen, Ezgi; Coşkun, Funda; Ediger, Dane; Uzaslan, Esra; Yöyen-Ermiş, Diğdem; Karaca, Mert; Terzi, Orkun; Bayram, Merve; Ömer, Dilara; Yiğitliler, Büşra; Yurttaş, Ahmet; Maharramov, Shahriyar; Çelik, Gamze; Oral, Barbaros; Karadağ, Mehmet; ACET ÖZTÜRK, NİLÜFER AYLİN; URSAVAŞ, AHMET; GÖREK DİLEKTAŞLI, ASLI; DEMİRDÖĞEN, EZGİ; COŞKUN, NECMİYE FUNDA; EDİGER, DANE; UZASLAN, AYŞE ESRA; YÖYEN ERMİŞ, DİĞDEM; KARACA, MERT; TERZİ, ORKUN ERAY; BAYRAM, MERVE; ÖMER TOPÇU, DİLARA; YURTTAŞ, AHMET; ORAL, HALUK BARBAROS; KARADAĞ, MEHMET; MAHARRAMOV, SHAHRİYAR; YAZICI, GAMZE; Bursa Uludağ Üniversitesi/Tıp Fakültesi.; 0000-0002-6375-1472; 0000-0002-7400-9089; 0000-0003-3604-8826; 0000-0002-2954-4293; 0000-0001-5871-8769; 0000-0002-9027-1132; AAG-8744-2021; JPK-7012-2023; AAH-3888-2021; AAE-9142-2019; AAI-3169-2021; AAD-1271-2019Item Lymphocyte subsets and cytokines in ascitic fluid of decompensated cirrhotic patients with and without spontaneous ascites infection(Wiley, 2006) Kıyıcı, Murat; Nak, Selim Giray; Budak, Ferah; Gürel, Selim; Oral, Barbaros; Dolar, Enver; Gülten, Macit; Uludağ Üniversitesi/Tıp Fakültesi/Gastroenteroloji Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/İmmünoloji Anabilim Dalı.; 0000-0003-0463-6818; 0000-0002-3208-6211; F-4657-2014; K-7285-2012; AAI-4213-2021; AAG-9177-2021; 6507627491; 6603336505; 6701913697; 7003706434; 7004498001; 6602075084; 6603629209Background and Aims: Spontaneous ascites infection is a frequently encountered and important complication of decompensated liver cirrhosis. The immune system plays an important role in the development or eradication of this infection. A number of compositional and functional alterations in immune system cells have been demonstrated in cirrhotic patients; however, there is a lack of knowledge about this issue in ascitic infections. The aim of the present study was to evaluate lymphocyte subsets and levels of some ascitic and lymphocytic intracytoplasmic cytokines in decompensated cirrhotic patients with or without spontaneous ascites infection. Methods: The study population consisted of 45 decompensated cirrhotic patients (32 men, 13 women) with different etiologies. Patients with ascitic polymorphonuclear leukocyte count >= 250/mm(3) and/or positive ascitic bacterial cultures were classified as the 'infected group'. Comparison was made between the infected and non-infected group for the following parameters: ascites leukocyte counts and differentiations; ascitic fluid protein; albumin levels and serum-ascites albumin gradients; flow cytometric detection of cell surface markers for ascitic T, B and natural killer lymphocytes; intracytoplasmic interleukin (IL)-2, IL-4, tumor necrosis factor (TNF)-alpha and interferon (IFN)-gamma; levels of ascitic IL-8, IL-10, IL-12 and TNF-alpha; and soluble Fas antigen and soluble Fas ligand. Results: The CD4/CD8 ratio was significantly decreased and expression of T cell receptor-gamma delta was increased in the infected group. Furthermore, ascites TNF-alpha levels were also elevated in this group. Ascitic IL-8, IL-10, IL-12 and TNF-alpha levels were significantly higher in patients with positive ascitic bacterial culture. Conclusions: These results suggest that a cytotoxic, especially Th1, immune response predominates in ascites infections. It also demonstrates that TNF-alpha might be involved in the pathogenesis of ascites infections.Item Matrix metalloproteinase-9 (MMP-9) elevated in serum, but not in bronchial lavage in lung cancer patients(Europen Respiratory Soc Journal, 2013-09-01) Koç, Melike; Ediger, Dane; Budak, Ferah; Karadağ, Mehmet; Oral, Barbaros; Uzaslan, Esra; Ege, Ercüment; Gözü, Oktay; Uludağ Üniversitesi/Tıp Fakültesi/Pulmonoloji, Alerji Anabilim Dalı.; Uludağ Üniversitesi/Veterinerlik Fakültesi/Tıbbi ve Klinik Mikrobiyoloji Anabilim Dalı.; 0000-0002-9027-1132; 0000-0001-7625-9148; 0000-0003-0463-6818; AAI-1004-2021; K-7285-2012; F-4657-2014; AAG-8744-2021; AAE-9142-2019Item Serum monocyte chemoattractant protein-1 and monocyte adhesion molecules in type 1 diabetic patients with nephropathy(Elsevier Science, 2006) Kıyıcı, Sinem; Ertürk, Erdinç; Budak, Ferah; Ersoy, Canan; Tuncel, Ercan; Duran, Cevdet; Oral, Barbaros; Sığırcı, Deniz; İmamoğlu, Sazi; Uludağ Üniversitesi/Tıp Fakültesi/Endokrinoloji ve Metabolizma Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/İmmünoloji Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Biyoistatistik Anabilim Dalı.; 0000-0003-0463-6818; F-4657-2014; K-7285-2012; AAJ-6536-2021; AAH-8861-2021; AAB-6174-2020Background. Monocyte chemoattractant protein (MCP)-1 is suggested to be implicated in the pathogenesis of diabetic nephropathy by activating and recruiting monocytes to the glomerulus via regulation of adhesion molecule expressions. The aim of this study was to test potential associations between serum concentrations of MCP-1, monocyte expression of Mac-1 and LFA-1 and nephropathy in type 1 diabetes mellitus. Methods. Serum MCP-1 levels and expression of monocyte adhesion molecules in 51 type 1 diabetic patients with and without diabetic nephropathy were compared with matched 15 healthy control subjects. Concentrations of serum MCP-1 were determined by enzyme-linked immunosorbent assays whereas monocyte expression of adhesion molecules Mac-1 and LFA-1 was measured by flow cytometry. Results. Serum MCP-1 levels and expression of Mac-1, but not LFA-1, were significantly higher in diabetic patients compared with controls. The mean serum MCP-1 level was 137.2 +/- 71.4 pg/mL in control patients, whereas it was 246.2 +/- 114.9 pg/ml in diabetic patients (p = 0.002). Serum MCP-1 levels were positively correlated with HbA1c and plasma fasting glucose levels. There was no difference in serum levels of MCP-1 and expression of monocyte adhesion molecules between type 1 diabetic patients with and without diabetic nephropathy. Conclusions. In type 1 diabetic patients, the levels of circulating MCP-1 concentration and expression of Mac-1 is mostly influenced by glycemic control rather than the existence of diabetic nephropathy.Item Value of serum and bronchoalveolar fluid lavage pro- and anti-inflammatory cytokine levels for predicting bronchopulmonary dysplasia in premature infants(John Libbey Eurotext, 2012-06) Köksal, Nilgün; Kayık, Bülent; Çetinkaya, Merih; Özkan, Hilal; Budak, Ferah; Kılıç, Şebnem; Canıtez, Yakup; Oral, Barbaros; Uludağ Üniversitesi/Tıp Fakültesi/Çocuk Sağlığı ve Hastalıkları Anabilim Dalı/Neonatoloji Bölümü.; Uludağ Üniversitesi/Tıp Fakültesi/Mikrobiyoloji ve Enfeksiyon Hastalıkları Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Çocuk Sağlığı ve Hastalıkları Anabilim Dalı/Çocuk İmmünolojisi Bölümü.; Uludağ Üniversitesi/Tıp Fakültesi/Çocuk Sağlığı ve Hastalıkları Anabilim Dalı/Çocuk Alerjisi Bölümü.; 0000-0003-0463-6818; 0000-0001-8571-2581; F-4657-2014; AAH-1658-2021; AAG-8393-2021; K-7285-2012; 7003323615; 36113950300; 23994946300; 16679325400; 6701913697; 34975059200; 8988954700; 7004498001Objective: The aim of this study was to determine the value of pro- and anti-inflammatory cytokine levels in both blood and tracheal aspirate (TA) samples that were obtained within 24 h after birth for predicting bronchopulmonary dysplasia (BPD) development in premature infants. Material and methods: Premature infants, who were born before 32 weeks of gestation, weighing less than 1,500 g, and admitted with respiratory distress between September 2009 and December 2010, were enrolled. Tracheal aspirate samples and serum were obtained from all infants on the first day of admittance for evaluation of pro- and anti-inflammatory cytokine levels using ELISA. Results: The study included 102 premature babies of whom 31 (30%) had BPD diagnosed in the follow-up. Mild, moderate and severe BPD was diagnosed in 10 (32%), 14 (45%) and seven (23%) infants, respectively. Both serum and TA sample pro- inflammatory cytokine (TNF-alpha, IL-1 beta, IL-6) levels were significantly higher, and anti-inflammatory cytokine (IL-10) levels were significantly lower in infants who developed BPD compared with those who had no BPD. No significant differences were detected in either serum or TA sample pro- and anti-inflammatory cytokine concentrations in preterm infants with BPD in terms of BPD severity. Cut-off values of both serum and TA sample pro- and anti-inflammatory cytokine concentrations for predicting BPD were also determined. Conclusion: It is suggested that higher serum and TA pro- inflammatory cytokine (TNF-alpha, IL-1 beta, IL-6) concentrations, along with lower anti-inflammatory cytokine (IL-10) concentrations, might be used for predicting the development of BPD in premature infants with respiratory distress at birth.