Browsing by Author "Mecitoğlu, Zafer"
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Item Abomasum deplasmanlı ineklerde glukoz metabolizmasının değerlendirilmesi(Uludağ Üniversitesi, 2010-05-20) Mecitoğlu, Zafer; Şentürk, Sezgin; Uludağ Üniversitesi/Sağlık Bilimleri Enstitüsü/Veteriner İç Hastalıkları Anabilim Dalı.Sunulan çalışmada süt sığırlarında genellikle doğum sonrasında görülen ve insidansı giderek artan abomasumun sola deplasmanlarının etiyolojisinde glukoz metabolizması ve insülin direncinin rolünün araştırılması amaçlanmıştır.Çalışmanın materyalini, aynı işletmeye ait, aynı ortalama yaş, laktasyon sayısı, buzağı doğum ağırlığı, verim grupları, beslenme durumları ve vücut kondüsyonlarına sahip 20 adet sağlıklı ve 20 adet sola abomasum deplasmanlı (LDA) inek oluşturmuştur. Her iki gruptaki ineklerden, klinik muayenelerini takiben kan örnekleri alınarak hematolojik (total lökosit, nötrofil, lenfosit, monosit, eozinofil, bazofil, eritrosit, hemoglobin, hematokrit, MCH, MCHC, trombosit, PCT) ve serum biyokimyasal (albumin, ALP, ALT, amilaz, toplam bilirubin, BUN, Ca, P, kreatinin, globulin, Na, K, TP, glukoz, insülin, fruktozamin, GGT, AST, BHBA) parametreleri karşılaştırılmıştır.Hematolojik muayene sonucunda, total lökosit (p<0,001), nötrofil (p<0,01), lenfosit (p<0,001), monosit (p<0,001), eozinofil (p<0,01), eritrosit (p<0,01), hemoglobin (p<0,001), hematokrit (p<0,001), MCH (p<0,05), MCHC (p<0,05) ve serum biyokimyasal muayenesi sonucunda da albumin (p<0,05), kalsiyum (p<0,05), GGT (p<0,001), AST (p<0,001) ve BHBA (p<0,01) değerlerinde iki grup arasında istatistiksel bir fark belirlenmesine karşın, glukoz metabolizması ile ilişkili olarak glukoz, insülin ve fruktozamin değerlerinde istatistiksel olarak herhangi bir fark tespit edilememiştir.Çalışma sonuçları temelinde, abomasum deplasmanlı hayvanlarda serum glukoz, insülin ve fruktozamin seviyelerinin kontrol grubu ile yakın değerlerde bulunmasına karşın, abomasum deplasmanlı hayvanlarda yüksek olan BHBA değerleri, bu gruptaki ineklerde insülin direncinin varlığını gösterebilir. Çalışmada yine insülin direnci ile yakından ilişkili olan karaciğer fonksiyonlarında, abomasum deplasmanlı hayvanlar ile sağlıklı hayvanlar arasında belirgin bir fark belirlenmiştir. Abomasum deplasmanlarında insülin direnci, glukoz metabolizması ve karaciğer yağlanmasının rolünün araştırıldığı farklı çalışmaların yapılması, abomasum deplasmanlarının etiyolojisinde yeni bir pencerenin açılmasına ve abomasum deplasmanlarının oluşmadan önce belirlenerek önlenebilmesine olanak sağlayabilir. Bununla birlikte abomasum deplasmanlarında, karaciğer profilinin değerlendirilmesi ile, karaciğer yağlanmasının varlığı ve abomasum deplasmanlarının prognozuna etkisi belirlenebilir.Item Bir alman çoban köpeğinde sertoli hücre tümörü ve seminoma nedenli feminizasyon sendromu(Uludağ Üniversitesi, 2014-12-30) Salcı, Hakan; Akdeşir, Ezgi; Çatık, Serkan; Şen, İsmail Altuğ; Salcı, E. Sinem Özdemir; Mecitoğlu, Zafer; Uludağ Üniversitesi/Veteriner Fakültesi.Yedi yaşlı, erkek bir Alman Çoban köpeği testislerinde şişkinlik, meme başlarında genişleme ve tüy dökülmesi şikayetleriyle kliniklerimize getirildi. Klinik muayenede feminizasyon bulguları (alopecia, deri renginde koyulaşma, meme başlarında büyüme) ve skrotal şişkinlik saptandı. Laboratuar muayenesi kronik yangısal bir hastalığı işaret etti. Ultrasonografik olarak karışık ekojenitede solit bir testiküler kitle saptadı ve diğer organlarda metastaz yoktu. Testis tümörü tanısı konulan hastada, genel anestezi altında bilateral orşiektomi operasyonu yapıldı. Çıkarılan testislerin histopatolojik muayenesinde seminoma ve sertoli hücre tümörü ile uyumlu bulgular saptandıItem Changes in circulating adiponectin and tumour necrosis factor-alpha and their relationship with insulin resistance in periparturient dairy cows(Sciendo, 2016-05-13) Mecitoğlu, Zafer; Şentürk, Sezgin; Akgül, Gülsah; Udum, Duygu; Uzabacı, Ender; Kasap, Sevim; Çatık, Serkan; Uludağ Üniversitesi/Veteriner Fakültesi/İç Hastalıkları Anabilim Dalı.; Uludağ Üniversitesi/Veteriner Fakültesi/Biyokimya Anabilim Dalı.; Uludağ Üniversitesi/Veteriner Fakültesi/Biyoistatistik Anabilim Dalı.; 0000-0001-7052-1694; 0000-0002-2465-9913; 0000-0002-5557-121X; 0000-0002-9634-0055; AAH-5167-2021; AAH-5069-2021; AAH-1677-2021; AAP-7998-2020; 36457647300; 56257771200; 56604015400; 31967936400; 55347697800; 36514844800; 56515323800Introduction: The aim of the study was to investigate changes in the serum levels of adiponectin and TNF-alpha, as well as insulin sensitivity, and to elucidate the possible relationship among the parameters and negative energy balance during the periparturient period of dairy cows. Material and Methods: Thirty primiparous Holstein dairy cows were selected for the study. Blood samples were collected from each cow seven days before the expected calving date, on the calving day, and 7, 14, and 21 days after calving. Blood non-esterified fatty acids (NEFA), beta-hydroxybutyric acid (BHBA), glucose, insulin, adiponectin, and TNF- alpha levels were measured. Revised Quantitative Insulin Sensitivity Check Index (rQUICKI) was calculated using data on NEFA, insulin, and glucose concentrations. Results: When compared to prepartum levels, serum concentration of adiponectin significantly increased on day 21 postpartum. The rQUICKI increased and NEFA levels decreased on day 7 after parturition. Insulin and glucose levels decreased on days 7, 14, and 21 postpartum when compared with prepartum levels. BHBA levels decreased on day 21 and TNF- alpha concentration also decreased on days 7, 14, and 21 postpartum. Adiponectin levels positively correlated with NEFA during the preparturient period. Negative correlation was detected between adiponectin and rQUICKI on calving day and on 14th day after parturition. TNF- alpha concentration positively correlated with glucose levels on day 7 prepartum and on 21st day postpartum and with rQUICKI on 21st day postpartum. Negative correlation was detected between adiponectin level and insulin sensitivity. Conclusion: Based on the results of the study, we concluded that adiponectin could possibly increase insulin sensitivity when blood NEFA concentrations are elevated.Item Clinical and biochemical evaluation of cows occurring severe weight loss after calving(Uludağ Üniversitesi, 2010-04-05) Şentürk, Sezgin; Mecitoğlu, Zafer; Temizel, Mutlu; Cihan, Hüseyin; Kasap, Sevim; Demir, Gülşah; Uludağ Üniversitesi/Veteriner Fakültesi.This study was designed to observe the biochemical and clinical changes in Holstein cows suffering from severe weight loss after calving. Clinically, out of the 75 milking cows in the first 8 week postpartum examined, 25 cows aged 4– 8 years were found to be suffering from decreased milk production, severe body weight loss. 25 healthy cows in the same period after calving, aged 4 – 8 years, were also used as a control group. Serum biochemistry analyses including potassium (K), sodium ( Na), calcium (Ca), phosphorus (P), magnesium (mg), albumin, alkaline phosphatase (ALP), gamma-glutamyltransferase (GGT), aspartate aminotransferase (AST), total biluribin, (TBIL) total protein (TP), creatinine (Cre), blood urea nitrogen (BUN), and β-Hydroxybutyrate (BHBA) were evaluated. Body condition score (BCS) of 17 of 25 cows, severe weight loss after calving cows, was ≤ 2.0 and BCS of 8 cows was between 2.0 and 3.0. BCS of Animals in the control group were determined as range: 3.5 -4.0. Endometritis in 11 cows, subclinical mastitis in 8 cows, clinical ketosis in 5 cows, sub clinical ketosis in 20 cows, and left displaced abomasum in 3 cows were determined. Identical points of 25 animals were higher BHB, GGT, AST, total globulin, and total protein levels according to healthy cows and references values. In addition, these cows had hypoalbunemi and hypocalcaemia, when compared with healthy cows. The results of the study reported here indicated that metabolic profile including serum BHBA, calcium, albumin, GGT, AST, total protein, should be evaluated to determine the causes of severe weight loss, and decreased yield in cows after calving.Publication Comparing the effect of ammonium molybdate versus ammonium molybdate and menbutone on hepatic functions of sheep with subclinical copper poisoning(Wiley, 2022-09-27) Kaçar, Yiğit; Mecitoğlu, Zafer; Batmaz, Hasan; KAÇAR, YİĞİT; MECİTOĞLU, ZAFER; BATMAZ, HASAN; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/İç Hastalıkları Anabilim Dalı; AAH-4972-2021; FQB-3477-2022; HXD-1722-2023This study aimed to investigate the effect of using menbutone in addition to ammonium molybdate on liver enzymes in sheep naturally poisoned with copper. Merino lambs (n = 30), naturally poisoned with copper and which also had high liver enzyme levels, were divided into two groups, each with 15 lambs. The AM + MEN group received ammonium molybdate and menbutone and the AM group received only ammonium molybdate solution. Both groups received 1.7% ammonium molybdate solution (1 mL per 10 kg body weight [BW]) subcutaneously on 0, 2nd and 4th days of the study. Menbutone (Genabil (R), Boehringer Ingelheim, Germany) was administered intramuscularly at a dose of 10 mg/kg BW on days 0 and 2, in addition to ammonium molybdate in the AM + MEN group. Blood samples were collected on days 0 and 7, and aspartate aminotransferase (AST), gamma-glutamyltranspeptidase (GGT) and creatinine levels were evaluated. Over 7 days, AST levels decreased from 351.04 +/- 63.50 IU/L to 286.40 +/- 55.68 IU/L in the AM group (P > 0.05) and from 425.00 +/- 119.25 IU/L to 240.83 +/- 29.62 IU/L in the AM + MEN group (P <= 0.05). GGT levels decreased from 121.16 +/- 15.88 IU/L to 110.39 +/- 10.13 IU/L in the AM group (P > 0.05) and 124.52 +/- 15.50 to 98.60 +/- 9.08 IU/L in the AM + MEN group (P <= 0.05). Based on these findings, the use of menbutone, in addition to ammonium molybdate, has significantly reduced the level of liver enzymes.Item Comparing the effects of treatment with ammonium molybdate versus ammonium molybdate and phenoxy-2-methyl-2-propionic acid on liver functions in natural copper poisoning of sheep(Elsevier, 2017-03-11) Mecitoğlu, Zafer; Topal, Onur; Kaçar, Yiğit; Batmaz, Hasan; Uludağ Üniversitesi/Veteriner Fakültesi/Dahiliye Anabilim Dalı.; 0000-0002-5557-121X; 0000-0002-1933-7354; 0000-0002-8389-4833; 0000-0003-1991-8957; AAH-1677-2021; S-8278-2017; AAH-4972-2021; AAH-1712-2021; 36457647300; 56548777100; 57193763918; 6602783183Aim of the presented study is to compare the effects of treatment with ammonium molybdate versus ammonium molybdate and phenoxy-2-methyl-2-propionic acid on liver functions in natural copper poisoning of sheep and overall treatment responses in sheep naturally poisoned with copper (Cu). Study was conducted on 80 yearlings aging between 6-9 months. AM + PMPA group (n =50) received ammonium molybdate and PMPA and AM group (n =30) received only ammonium molybdate. First blood samples were collected before the treatments. PMPA was administered once daily intramuscularly at dose of 10 mg/kg for the first three days of the study to AM + PMPA group. AM + PMPA and AM groups both received ammonium molybdate two times with one week interval at dose of 1.34 mg/kg (1 cc per 10 kg BW, of %1.34 ammonium molybdate in saline solution) subcutaneously. Second blood samples were collected from all 80 animals on day 21 of the study. Cu levels were measured in a subgroup of randomly selected 9 (5 from AM + PMPA and 4 from AM group) animals on days 0 and 21 of the study. Mean Cu levels were 158.25 +/- 14 mu g/dl and 156.75 +/- 9 mu g/dlon day 0 and 129 +/- 9 mu g/dl and 154.5 +/- 22 mu g/dl in AM + PMPA and AM group respectively. AST levels decreased from 502 +/- 67.2114 to 168 +/- 10.1 IU/L in AM + PMPA group (P < 0.001) and from 423 +/- 71.1 IU/Lto 202 +/- 17.1 IU/L in AM group (P = 0.005) on day 21 of the study. GGT levels were 250 +/- 24.2 IU/L and 248 +/- 28.1 IU/L on day 0 and decreased to 160 +/- 16.41 U/L and 166 +/- 22.2 IU/L on day 21 in AM + PMPA and AM group with significance of P=0.001 and P=0.037 respectively. Two animals from AM group and one from AM + PMPA group died during the study period. Based on the more pronounced decrease in AST and GGT levels in AM + PMPA group we conclude that PMPA has beneficial effects on liver functions in chronic copper poisoning of sheep probably as a result of decreased lipid peroxidation in hepatocytes and/or increased Cu elimination by cholerectic effects of PMPA.Item Comparison of IgG and semiquantitative tests for evaluation of passive transfer immunity in calves(Bilimsel ve Teknolojik Araştırma Kurumu, 2018-04-22) Topal, Onur; Batmaz, Hasan; Mecitoğlu, Zafer; Uzbacı, Ender; Uludağ Üniversitesi/Veteriner Fakültesi/İç Hastalıkları Anabilim Dalı.; Uludağ Üniversitesi/Veteriner Fakültesi/Biyoistatistik Anabilim Dalı.; 0000-0002-1933-7354; 0000-0003-1991-8957; 0000-0002-5557-121X; 0000-0002-9634-0055; S-8278-2017; AAH-1712-2021; AAH-1677-2021; 56548777100; 6602783183; 36457647300; 55347697800Serum immunoglobulin (IgG) and semiquantitative tests are used for the evaluation of passive transfer immunity (PTI) in calves. We aimed to evaluate PTI in calves by using a Brix refractometer, total protein (TP), gamma-glutamyl transferase (GGT), and glutaraldehyde coagulation test (GCT) on days 1, 3, and 7 after birth; to compare the results with serum IgG; and to evaluate which day these tests are given on will give the best results. The blood samples were collected from 60 Holstein dairy calves on day 0, just after birth, and on the 1st, 3rd, and 7th days after birth. The serum IgG concentration was measured by ELISA, the Brix % and TP concentrations with refractometers, and GGT activity using a dry chemistry system. The duration of the GCT was determined in the first 60 min. The IgG, TP concentration, and Brix % all peaked on the 3rd day of the study. GGT showed a significant decline after 24 h. Evaluating the Brix %, IgG, TP, and GCT levels more than 24 h after birth gives better results. However, GGT activity was observed as an early indicator of failure of passive transfer, as the GGT levels were highest 24 h after birth.Item Comparison of two cow side bhba tests for diagnosis of subclinical ketosis(Uludağ Üniversitesi, 2012-06-11) Demir, Gülşah; Mecitoğlu, Zafer; Çatık, Serkan; Şentürk, Sezgin; Uludağ Üniversitesi/Veteriner Fakültesi.Ketosis is a disease of high yielding dairy cows caused by negative energy balance. Subclinical ketosis is a type of disease in which keton bodies in blood are elevated but not enough to cause clinical findings. Economic impact of subclinic ketosis makes disease one of the most important problems of dairy industry. Quick and accurate diagnosis of sublinical ketosis is crucial for economic development of a dairy herd thus in presented study two cowside BHBA test were compared according to two different blood BHBA cutoff values. Sensitivities were found to be 58%, 50% and specifities were 84%, 93% respectively for cut off points of 1.2 and >1.4mmol/L. overall specifity and sensitivity levels calculated indicates that Precision Xtra™ could be used for detection of blood BHBA levels in suspected cows at the same time Precision Xtra™ is quicker than Ketosite analysis takes 20 seconds per sample however with Ketosite one analysis takes about 2 minutes.Item Determination of the effect of a monensin capsule (continuous-release capsules) on metabolic parameters in transition dairy cows(Polish Society Veterinary Sciences, 2020-06-01) Ertürk, Melih; Kasap, Sevim; Mecitoğlu, Zafer; Dülger, Hüseyin; Babaeski, Serdar; Kennerman, Engin; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Dahiliye Anabilim Dalı.; 0000-0002-5557-121X; AAH-1677-2021; 36514844800; 36457647300; 55376821600; 57211806818; 16031244000Monensin is an ionophore antibiotic that changes the population of rumen bacteria. The aim of this study was to determine the effects of a monensin controlled-release capsule administered 3 weeks before calving on blood metabolites (serum beta-hydroxybutiric acid (BHBA), cholesterol (CHOL), urea, creatinine (CREA), gamma glutamyl transferase (GGT), aspartate aminotransferase (AST), total protein (TP), phosphorus (P), magnesium (Mg+), calcium (Ca++), glucose (GLU) and plasma non-esterified fatty acids (NEFA) concentration) and milk yield before and after calving. A total of 50 Holstein-Friesian cows were selected from the same flock. Blood samples were taken 3 weeks before the expected calving date and during weeks 1, 2, 4, 6, and 8 after calving. The cows were divided into two groups: a study group (Group M, n = 40) and a control group (Group C, n = 10). Group M received orally a cylindrical device (Kexxtone, ELANCO Animal Health, Guelph, ON, Canada), and group C received no treatment. BHBA and NEFA were lower (P < 0.05) in group M compared with group C in weeks 6 and 8 after calving. GLU was higher (P < 0.05) in group M compared with group C in week 4 after calving. Urea was higher (P < 0.05) in group M compared with group C in week 1 after calving. There was no statistically significant difference (P <0.05) between the two groups in milk yields. Although milk yield was similar in both groups, the changes in the concentrations of BHBA, NEFA, GLU and urea show that a monensin CRC could be used as a glycogenic precursor.Item Effect of levamisole on faecal levels of acid-fast organisms in cows with paratuberculosis(Wiley, 2009-07-25) Şentürk, Sezgin; Mecitoğlu, Zafer; Ülgen, Mihriban; Önat, Kaan; Uludağ Üniversitesi/Tıp Fakültesi/Dahiliye Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Mikrobiyoloji Anabilim Dalı.; 0000-0002-2465-9913; 0000-0002-5557-121X; 0000-0002-6307-5179; AAH-5069-2021; AAH-1677-2021; AAG-8117-2021; 56257771200; 36457647300; 56259664500; 24069071700Item Effects on negative energy balance of tannin in dairy cattle(Uludağ Üniversitesi, 2015-08-25) Şentürk, Sezgin; Cihan, Hüseyin; Kasap, Sevim; Mecitoğlu, Zafer; Temizel, Mutlu; Uludağ Üniversitesi/Veteriner Fakültesi.Tannins are polyphenolic compounds. Tannins are chemically divided into two main groups as hydrolysable tannins and condensed tannins. Tannins are used in ruminants in order to prevent ruminal tympani and the formation of high methane gas. In the present study, it was aimed to determine the effects of tannin on of negative energy balance metabolism in dairy cattle. Animals, after the physical examination, were divided into 2 groups as study (test group n = 10) and control group (n = 10). Herbal extract (Quebracho Colorado) of tannin (Unitan Saica Inc., Argentina) which is appropriate for animal use, was applied 90 gr per animal daily for 6 weeks (each 3 weeks in pre and postpartum period) to animals in study group. While no any tannin or other additive was given to the animals in control group. Faeces, blood and milk (only after parturition on days 7, 14 and 21) samples were collected from both group on 21st day before parturition, during parturition and 7th, 14th, and 21st day from parturition. Beta hydroxybutyrate (BHB), albumin, calcium, phosphorus, total protein, BUN, GGT, cholesterol and triglycerides from blood samples and milk urea nitrogen (MUN) levels from milk samples were evaluated. Difference between the groups for BUN, MUN, calcium, phosphorus, triglycerides, albumin, total protein and GGT was not detected. However, a decline was detected for BHB levels during parturition, 7th, 14th (not statistically significant) and 21st (statistically significant) days after parturition in the study group compared to the control group. As a result; in animals, as detected lower BHB levels in animals applied tannin in study group compared to the control group, tannin can be used for the protection against negative energy balance.Item Evaluation of faecal shedding of acid-fast mycobacterium avium subsp. paratuberculosis (map) in both intradermal johnin test- and serologically (Elisa) mappositive cattle(Uludağ Üniversitesi, 2012-11-22) Cihan, Hüseyin; Mecitoğlu, Zafer; Demir, Gülşah; Temizel, Ethem Mutlu; Şentürk, Sezgin; Uludağ Üniversitesi/Veteriner Fakültesi.Paratuberculosis is a debilitating disease of livestock caused by Mycobacterium avium (MAP). As a result of economic impact caused, disease is now one of the most important problems of dairy industry. Detection of animals shedding MAP organism plays a crucial role in herd eradication of the disease. Aim of the presented study was to evaluate relationship between MAP ELISA results, Intradermal Johnin test and fecal MAP shedding ratio. 40 ELISA and Johnin test positive animals were selected for the study. 25 of 40 animals (62.5%) were shedding MAP organisms with their feces. Results of the present study indicate that combination ELISA and Johnin test results is a useful tool for prediction of animals shedding MAP.Publication Evaluation of serum choline along with, some biochemical and clinical parameters in cattle suffering from with botulism(Pakistan Agricultural Scientists Forum, 2021-02-01) Batmaz, Hasan; Mecitoğlu, Zafer; Koç, Cansu; Kaya, Fatih; Topal, Onur; Cansev, Mehmet; BATMAZ, HASAN; MECİTOĞLU, ZAFER; KOÇ, CANSU; KAYA, FATİH; TOPAL, ONUR; CANSEV, MEHMET; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/İç Hastalıkları Anabilim Dalı; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Farmakoloji Anabilim Dalı; 0000-0002-6097-5585; 0000-0001-8820-1509; 0000-0002-1933-7354; 0000-0003-2918-5064; M-9071-2019; AAA-4754-2022; IXN-7700-2023; S-8278-2017; HXD-1722-2023; FQB-3477-2022Botulism is a disease of cattle that causes significant impact due to its high mortality rate. The aim of the present study is to evaluate serum choline levels as well as clinical and biochemical parameters of cattle suspected to be suffering from botulism and to compare the results with healthy cattle and also to compare the results of survivors and fatalities. Thirteen botulism suspected and eleven healthy cattle were used. Total protein, albumin, aspartate aminotransferase (AST), alanine aminotransferase (ALT), gamma glutamyl transferase (GGT), calcium, phosphorus, magnesium and potassium levels were measured from the sera of botulism and control cattle by colorimetric method using an autoanalyser. Choline levels were analyzed using HPLC. Dysphagia, decreased tongue strength weak anal reflexes, tongue and tail paralysis and locomotion disorders were the main clinical findings observed in suspected botulism cases. Botulinum toxin analysis of ruminal fluid samples were positive in two cattle from one herd. AST, ALT, potassium and choline levels were higher in cattle suffering from suspected botulism cases and choline levels were positively correlated with albumin and total protein levels. Three animals from botulism group survived. Clinical findings were milder, animals were not recumbent and choline levels were lower in survivors. Clinical findings of the three animals that recovered were milder, animals were not recumbent and choline levels were lower in survivors. It was observed that clinical findings such as decreased tongue strength and anal reflexes, dysphagia, tail paralysis, and locomotion disorders are important for diagnosis of botulism In conclusion, cattle with milder clinical signs and lower choline have a higher chance of survival.Item Evaluation of serum iron and iron binding capacity in cows with paratuberculosis(George Thieme, 2009) Şentürk, Sezgin; Mecitoğlu, Zafer; Ülgen, Mihriban; Borum, Ebru; Temizel, Ethem Mutlu; Kasap, Sevim; Uludağ Üniversitesi/Veterinerlik Fakültesi/İç Hastalıkları Anabilim Dalı.; Uludağ Üniversitesi/Veterinerlik Fakültesi/Mikrobiyoloji Anabilim Dalı.; 0000-0002-2465-9913; 0000-0002-6307-5179; AAH-5069-2021; AAP-7998-2020; AAG-8117-2021; 56257771200; 36055033100; 56259664500; 36738850800; 26430270100; 36514844800Objective: The purpose of the study was to evaluate the results of routine haematological, serum iron and iron binding capacity analyses in cattle with Mycobacterium avium subsp. paratuberculosis (MAP) infection. Material and methods: Thirty-eight cows were included in the study. An ELISA for MAP, intradermal Johnin test and microscopic examination of the animals faecal smears for acid fast organisms were performed. According to the results 19 cows (4-8 years old) were found positive and 19 cows (4-7 years old) from the same herd were negative and served as control group. In all cows routine haematological values as well as serum iron level and iron binding capacity were evaluated. Results: When compared with control animals, routine haematological values including haematocrit, haemoglobin and erythrocyte counts were significantly (p < 0.05) lower in positive cows. Also serum iron level (p < 0,01) and iron binding capacity (p < 0.01) were significantly lower in positive cows than in the control group. Conclusion: As a result, these parameters can be important for the mechanism of the pathogenesis of paratuberculosis.Publication Immune response of cattle to botulinum type C and D toxoid administered on three occasions(Univ Agriculture, Fac Veterinary Science, 2015-01-01) Mecitoğlu, Zafer; Temizel, Ethem Mutlu; Özyigit, Özgür; Akgül, Gülşah; Kennerman, Engin; MECİTOĞLU, ZAFER; TEMİZEL, ETHEM MUTLU; ÖZYİĞİT, MUSA ÖZGÜR; Akgül, Gülşah; KENNERMAN, ENGİN; Uludağ Üniversitesi/Veteriner Fakültesi/Patoloji Anabilim Dalı; Uludağ Üniversitesi/Veteriner Fakültesi/Dahiliye Anabilim Dalı; AAH-1677-2021; ECV-4083-2022; GLP-3036-2022; CBU-1718-2022; FDU-5862-2022The aims of the present study were to investigate the antibody response of cows from an outbreak region to vaccination with a bivalent botulinum toxoid (Type C and D) on three occasions and to investigate the antibody response to vaccination with the botulinum toxoid in the presence of anti-BoNT antibodies. Twenty-two cows from an outbreak region and 10 cows from a farm without a history of botulism were used in this study. Blood samples were collected before each vaccination and three weeks after the third vaccination (days 0, 21, 42 and 63). The level of anti-BoNT/C antibodies steadily increased after each vaccination (0.471 +/- 0.04, 0.566 +/- 0.03 and 0.663 +/- 0.04, respectively); however, the levels of anti-BoNT/ D antibodies were not significantly different after the second and third vaccinations (0.377 +/- 0.03, 0.493 +/- 0.03 and 0.465 +/- 0.03, respectively). Post vaccination antibody responses of animals found positive and negative for anti-BoNT antibodies at the beginning of the study were similar. The results of the present study indicated that vaccination of cattle with botulinum toxoid on three occasions is recommended, particularly in outbreaks that are suspected to be caused by BoNT/C and that presence of naturally acquired antibodies against BoNT did not interfere with post vaccination immune response.Item Isolation of first local coranavirus from cattle with winter dysentery in Turkey(Uludağ Üniversitesi, 2014-04-30) Ertürk, Arife; Gülyaz, Veli; Gülaçtı, İrem; Özdemir, Sancak; Onat, Kaan; Akgül, Gülşah; Mecitoğlu, Zafer; Çatık, Serkan; Temizel, E. Mutlu; Şentürk, Sezgin; Şenlik, Bayram; Uludağ Üniversitesi/Veteriner Fakültesi.; Uludağ Üniversitesi/Veteriner Fakültesi/Parazitoloji Bölümü.Winter dysentery (WD) is a very contagious disease of cattle characterized by profuse diarrhea. The precise etiology of disease is still not fully elucidated. Aim of the presented study was investigation of the causative agents of disease in a herd affected by WD and to evaluate routine haematological and biochemical parameters of cows with WD. This study was carried out on blood and fecal samples collected from 12 cows showing typical signs of disease during an outbreak of WD in Bursa, Turkey. Samples were analysed for the presence of bovine coronavirus (BCoV), Bovine viral diarrhea (BVD), Eimeria oocysts and Campylobacter spp. using ELISA, RT-PCR, flotation technique and culture. Faecal samples were inoculated in HRT cell cultures for virus isolation. Isolated viruses were identified as coronaviruses from 25% of the samples by ELISA, PCR and serum neutralisation tests. Campylobacter spp. was isolated from 33.3% cows with dysentery. BVD and Eimeria oocysts were not detected in any of the samples. Results of the presented study indicates that BcoV along with Compylobacter spp. may be the primary agent of WD in cows. However negative results for BCoV and Campylobacter spp of 58 % of feacal samples indicates that etiology of the disease is still not fully elucidated and pathogens other than BCoV and Campylobacter could also be involved in pathogenesis of the diseaseItem Merinos ırkı bir koyunda iç organ tutulumlu kazeöz lenfadenitis olgusu(Uludağ Üniversitesi, 2013-04-19) İpek, Volkan; Akgül, Ömer; Kahraman, M. Müfit; Öztürkoğlu, Sevda İnan; Büyükcangaz, Esra; Mecitoğlu, Zafer; Mecitoğlu, Gülnaz Yılmazbaş; Uludağ Üniversitesi/Veteriner Fakültesi.Koyunlarda Corynebacterium pseudotuberculosis, kazeöz lenfadenitis (KLA) ve iç organlarda apselerle karakterize olan, kronik bir hastalıktır ve koyun yetiştirilen birçok ülkede rapor edilmiştir. Etken, pleomorfik, Gram pozitif ve makrofajlarda çoğalan bir bakteridir. Zayıf yün gelişimi, süt veriminde düşme, üremede bozukluklar nedeniyle önemli ekonomik kayıplara neden olmakta ve çoğunlukla tedaviye yanıt alınamamaktadır. Sunulan vakada iki yaşlı, solunum problemi şikâyeti ile getirilen Merinos ırkı bir koyunda gözlenen KLA olgusu klinik, mikrobiyolojik ve patolojik bulgular eşliğinde değerlendirildi. Nekropsi sonrasında alınan organ numunelerine rutin takip işlemleri uygulanarak mikroskobik incelemeler yapıldı. Mikrobiyolojik incelemede Gram boyama, GBL koyun kanlı agarı, BHI ve MacConkey agara ekim ve CAMP testi uygulandı ve C. pseudotuberculosis identifiye edildi. Mediastinal lenf yumrusunun muayenesinde lamella oluşumlu piyogranulomatöz reaksiyon gözlendi. Karaciğerin histopatolojik muayenesinde ise iyi sınırlanmış apse alanları mevcuttu. Sağ böbrekte ise sadece membranoproliferatif bir glomerulonefritis görüldü. Aşılamanın yanı sıra, serolojik tanı hastalıkla savaşmada çok önemli bir yer tutar; böylece belirlenen enfekte hayvanların ayrılması veya itlafı yoluyla hastalıkla mücadele edilebilir ve bu KLA’nın tam eradikasyonu için gerekli görülmektedir. Ülkemizde küçük ruminantlarda yaklaşık %63 oranında görülen bu hastalığa yönelik olarak, aşılama ve eradikasyon çalışmalarına daha fazla ağırlık verilmesi gerekmektedirItem Mycoplasma agalactiae ile doğal enfekte bir keçi işletmesinde tylosinin etkilerinin değerlendirilmesi(Uludağ Üniversitesi, 2011-04-05) Önat, Kaan; Temizel, E. Mutlu; Göçmen, Hüban; Mecitoğlu, Zafer; Kasap, Sevim; Ülgen, Mihriban; Uludağ Üniversitesi/Veteriner Fakültesi.; Uludağ Üniversitesi/Sağlık Bilimleri Enstitüsü.Bu çalışmada, Bursa Yenişehir’de Bulaşıcı Agalaksiya hastalığı semptomları gösteren bir keçi sürüsü bakteriyolojik ve serolojik olarak incelendi ve Tylosin tedavisi sonrasında etken saçılımı değerlendirildi. Bütün hayvanların klinik muayenelerinin yapılmasının ardından serolojik muayeneleri yapıldı. Klinik muayene sonucunda 10 adet keçinin Bulaşıcı Agalaksiya hastalığının semptomlarını gösterdiği tespit edildi. Çalışmalarda 110 keçinin kan serumları ELISA ile incelendi ve klinik bulgu gösteren 10 hayvan dâhil 31 keçide seropozitiflik belirlendi. Bu 10 keçinin beşinde eklem şişlikleri, diğer beşinde ise eklem şişliği olmaksızın topallık gözlendi. Eklemlerinde şişlik bulunan beş keçiden alınan eklem sıvıları bakteriyolojik olarak incelendi ve dördünden Mycoplasma agalactiae izole edildi. Semptom gösteren 10 keçiye 5 gün süre ile 10 mg/kg Tylosin® (Tylan®, Lilly Elanco, Türkiye) uygulaması yapıldı. Laktasyonu takiben bütün keçilere tekrar ELISA testi yapıldı ve 34 keçide seropozitiflik saptandı. Seropozitif keçilerden alınan 34 süt örneğinin 9’undan Mycoplasma agalactiae izole edildi. Tylosin tedavisi uygulanan 10 keçiden sadece birisinde süt ile etken saçıldığı saptandı. Sonuç olarak, Tylosin uygulamalarının M. agalactiae’nın laktasyon sonrasında süt ile saçılımını azaltması ve klinik bulgularının iyileşmesi yönünde olumlu etkilerinin olduğu kanısına varıldı.Item Outbreak of malignant oedema in sheep following vaccination with a multivalent clostridial bacterin-toxoid(Georg Thieme, 2010) Cihan, Hüseyin; Yalçın, Ebru; Mecitoğlu, Zafer; Şentürk, Sezgin; Uludağ Üniversitesi/Veterinerlik Fakültesi/Klinik Bilimler Bölümü.; 0000-0002-5557-121X; 0000-0002-2465-9913; AAH-1677-2021; ABH-3742-2020; AAH-5069-2021; 6602381681; 36778554000; 36457647300; 56257771200Item Prepartum urine pH as A predictor of left displacement of abomasum(Pakistan Agricultural Scientists Forum, 2016-04) Mecitoğlu, Zafer; Şentürk, Sezgin; Akgül, Gülşah; Uzabacı, Ender; Kara, Çağdaş; Uludağ Üniversitesi/Veteriner Fakültesi/Dahiliye Anabilim Dalı.; Uludağ Üniversitesi/Veteriner Fakültesi/Hayvan Besleme ve Beslenme Hastalıkları Anabilim Dalı.; Uludağ Üniversitesi/Veteriner Fakültesi/Biyoistatistik Anabilim Dalı.; 0000-0002-5557-121X; 0000-0002-9634-0055; AAH-1677-2021; 36457647300; 56257771200; 31967678500; 56604015400; 55347697800The aim of this study was to investigate the relationship between prepartum urine pH, blood ionized calcium, and blood pH levels and the incidence of left displacement of abomasum (LDA). Holstein dairy cows (n=115) in their second lactation were selected for the study. Urine and blood samples were collected seven days prior to the expected calving date, and urine pH, blood ionized calcium, and blood pH levels were measured. Of 115 cows, 13 (11%) had LDA at 15 (+/- 7) days postpartum. Urine pH (LDA group: 6.11; healthy control group: 6.65) and blood pH (LDA group: 7.27; healthy control group: 7.37) levels were lower (P<0.05) in the LDA group than in the healthy control group. The sensitivity of urine pH, blood ionized calcium, and blood pH was 46, 62, and 84.6%, respectively, with corresponding specificities of 80.2, 61, and 44.1%. The results revealed that the measurement of urine pH levels in the last days of the dry period represents an inexpensive and fast method for predicting LDA. Future studies should incorporate dry matter intake calculations and larger sample sizes to evaluate the usefulness of urine pH in predicting LDA in practice.