Browsing by Author "Coven, Fethiye"

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Detection of israel variant 2 (is/1494/06) genotype of infectious bronchitis virus in a layer chicken flock
(Ankara Universitesi, 2021-01-01) Ongor, Hasan; Timurkaan, Necati; Coven, Fethiye; Karabulut, Burak; Eroksuz, Hatice; Cetinkaya, Burhan; Çarlı, Kamil Tayfun; CARLI, KAMİL TAYFUN; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Mikrobiyoloji Anabilim Dalı.; 0000-0001-6045-8644
The aim of this study was to determine an infectious bronchitis (IB) infection, caused by an Israel variant 2 (IS/1494/06)-like IBV, in a layer chicken flock regularly vaccinated with vaccines containing IBV H120 and 4/91 strains. Mild respiratory symptoms, drop in egg production and soft-shelled eggs and eventually death were observed in a layer chicken flock. Clinical samples from four diseased chickens were examined for the detection and genotyping of IBV by virus isolation, a commercial real time reverse transcription polymerase chain reaction (rRT-PCR) and nucleotide sequencing. Both Israel variant 2 (IS-Var2) and 793/B serotypes were detected from samples by rRT-PCR, but sequencing results of a 345 bp part of S1 gene revealed that our IBV isolate, HFT-IBV, was IS/1494/06 (IS-Var2)-like with the 97.7% genetic similarity. These results suggested that immunity against vaccination with a combination of different genotypes (H120 and 4/91) could not be protective for IS-Var2 IBV field infection. In addition, identification of genotypes from the clinical samples, such as swabs and organ samples by commercial rRT-PCR assays failed to find correct IBV genotype responsible for the IB infection, whereas analysis of nucleotide sequencing of S1 gene of IBV as a gold standard test undoubtedly detected causative genotype of the infection. Also, the findings indicated that there is an urgent need for consider genotype- or protectotype-match vaccination strategies in the field to prevent vaccine- and IB-dependent economic losses of the poultry sector and logically protect chickens from IBV infection.
How do we use molecular knowledge in diagnosis and control of pandemic avian viruses?
(Aves, 2022-01-01) Ardıçlı, Özge; Demirbilek, Serpil Kahya; Coven, Fethiye; Çarlı, Kamil Tayfun; ARDIÇLI, ÖZGE; KAHYA DEMİRBİLEK, SERPİL; CARLI, KAMİL TAYFUN; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Mikrobiyoloji Anabilim Dalı.; 0000-0001-6077-0478; 0000-0003-1928-7630; AAG-7421-2021; E-3867-2010; CNE-1191-2022
Pandemic respiratory viruses of poultry have caused significant economic losses in the poultry industry since the 1930s, and molecular and genetic techniques are widely used for diagnosis and control of the infections. Knowledge of changes in the genetic and antigenic characteristics of the pandemic viruses during the time can be really important for human pandemic viruses such as severe acute respiratory syndrome virus-coronavirus-2 and human influenza virus. The use of these techniques plays a vital role in preventing the faulty results and the possible financial losses that may occur due to the limited findings obtained from conventional laboratory tests. In the light of this information, the purpose of this review is to provide an up-to-date assessment of the diagnosis and prevention of major respiratory viruses in poultry and a general and field-oriented scientific perspective that may be useful in the industry. In this context, current approaches for diagnosis and vaccination applications developed using molecular methods based on avian coronavirus infectious bronchitis virus, avian paramyxovirus-1 virus, and avian influenza virus, which are pandemic, are discussed, and solution suggestions for an effective fight are presented.